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AB243667

Anti-ATP8 antibody [1G3-1H11-1C3]

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(1 Publication)

Mouse Monoclonal ATP8 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MT-ATP8.

View Alternative Names

ATP8, ATPASE8, MTATP8, MT-ATP8, ATP synthase F(0) complex subunit 8, A6L, F-ATPase subunit 8

1 Images
Western blot - Anti-ATP8 antibody [1G3-1H11-1C3] (AB243667)
  • WB

Supplier Data

Western blot - Anti-ATP8 antibody [1G3-1H11-1C3] (AB243667)

Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and transferred to a PVDF membrane in CAPS buffer.

Right panel shows total protein.

All lanes:

Western blot - Anti-ATP8 antibody [1G3-1H11-1C3] (ab243667) at 2 µg/mL

Lane 1:

Mitochondria from cultured normal Control Human Dermal Fibroblasts Neonatal (HDFn)

Lane 2:

Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of Ethidium Bromide

Secondary

All lanes:

HRP-labeled Goat-anti-mouse IgG

Predicted band size: 8 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1G3-1H11-1C3

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MT-ATP8. The exact immunogen used to generate this antibody is proprietary information.

P03928

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein L
Purification notes
Protein L affinity chromatography from Fetal Bovine Serum-containing medium. Purity: Near homogeneity as assessed by SDS-PAGE and Coomasie Blue staining.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: HEPES, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP8 also known as MT-ATP8 is a mitochondrial protein encoded by the mitochondrial DNA. It functions as a part of the ATP synthase complex or complex V of the electron transport chain. This complex is responsible for ATP production through oxidative phosphorylation. ATP8 is a small protein with a molecular mass of approximately 10 kDa. It is highly expressed in tissues with high energy demand such as the heart liver and muscle.
Biological function summary

The protein plays an important role in maintaining the mitochondrial membrane potential and ATP synthesis. ATP8 is a component of the F0 sector of the ATP synthase complex which is important for proton translocation across the mitochondrial inner membrane. This translocating activity is essential for the synthesis of ATP from ADP and inorganic phosphate. As part of this complex ATP8 contributes to cellular energy metabolism and regulation.

Pathways

ATP8 is involved in the oxidative phosphorylation pathway and plays an integral role in energy metabolism. It works within the ATP synthase enzyme complex which collaborates with other complexes in the electron transport chain to produce ATP. Related proteins in this pathway include ATP6 and COX1 both important for efficient electron transfer and energy production.

ATP8 mutations or dysfunctions can relate to mitochondrial disorders such as Leigh syndrome and mitochondrial myopathy. These conditions arise from defects in mitochondrial energy production. The protein's interactions with ATP6 further highlight the interconnected nature of mitochondrial disorders as both proteins are involved in maintaining ATP synthase functionality. Understanding ATP8's role in these diseases helps in uncovering potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit 8, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed : 37244256).
See full target information MT-ATP8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular medicine (Berlin, Germany) 102:1285-1296 PubMed39210159

2024

TRAP1 modulates mitochondrial biogenesis via PGC-1α/TFAM signalling pathway in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppina Bruno,Michele Pietrafesa,Fabiana Crispo,Annamaria Piscazzi,Francesca Maddalena,Guido Giordano,Vincenza Conteduca,Marianna Garofoli,Almudena Porras,Franca Esposito,Matteo Landriscina
View all publications
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Product promise

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