Rabbit Polyclonal ATP9A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ATP9A aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ATP9A aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link O75110
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Plays a role in regulating membrane trafficking of cargo proteins, namely endosome to plasma membrane recycling, probably acting through RAB5 and RAB11 activation (PubMed:27733620, PubMed:30213940, PubMed:36604604). Also involved in endosome to trans-Golgi network retrograde transport (PubMed:27733620, PubMed:30213940). In complex with MON2 and DOP1B, regulates SNX3 retromer-mediated endosomal sorting of WLS, a transporter of Wnt morphogens in developing tissues. Participates in the formation of endosomal carriers that direct WLS trafficking back to Golgi, away from lysosomal degradation (PubMed:30213940). Appears to be implicated in intercellular communication by negatively regulating the release of exosomes (PubMed:30947313). The flippase activity towards membrane lipids and its role in membrane asymmetry remains to be proved (PubMed:30947313). Required for the maintenance of neurite morphology and synaptic transmission (By similarity).
Alternative names
ATPIIA, KIAA0611, ATP9A, Probable phospholipid-transporting ATPase IIA, ATPase class II type 9A
Recommended products
Rabbit Polyclonal ATP9A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ATP9A aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ATP9A aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link O75110
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP9A also known as ATPase phospholipid transporting 9A is a protein that acts as a phospholipid flippase involved in the movement of phospholipids across cellular membranes. This protein has a mass of approximately 125 kDa and is expressed in various tissues with notable presence in the brain and liver. ATP9A contains important structural domains that enable it to bind and hydrolyze ATP driving the translocation of phospholipids to maintain the asymmetry of lipid bilayers.
- Biological function summary
The ATP9A protein plays an important role in membrane trafficking processes which are essential for proper cellular communication and cargo transport. It does not operate alone; ATP9A often associates with other cellular components to drive vesicular trafficking events. The protein is vital in endosomal sorting contributing to the correct targeting of proteins and lipids within cells. Dysregulation in its activity can disrupt these processes pointing to its importance in maintaining cellular homeostasis.
- Pathways
The functions of ATP9A integrate into mechanisms like endocytosis and phospholipid metabolism. It has a connection with the phosphatidylinositol signalling pathway which is critical for vesicle transport and membrane dynamics. ATP9A interacts with proteins such as Rab11 a small GTPase involved in recycling endosomes reflecting its importance in endocytic recycling processes. These interconnected activities demonstrate ATP9A's role in regulating intracellular lipid distribution and movement.
- Associated diseases and disorders
ATP9A is particularly significant in oncological and neurological contexts. Alterations in its expression or function have been linked to cancer progression due to changes in cell migration and invasion linked to phospholipid dysregulation. Furthermore its involvement in proper neuromodulation in the brain connects ATP9A to neurodegenerative diseases. Relatedly ATP9A influences the activity of SNARE proteins important for synaptic vesicle fusion highlighting its broader impact on synaptic health and pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-ATP9A antibody (ab234873)
All lanes: Western blot - Anti-ATP9A antibody (ab234873) at 1/500 dilution
Lane 1: MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 119 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ATP9A antibody (ab234873)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATP9A (green) using ab234873 at 1/100 dilution in ICC/IF. Secondary antibody was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP9A antibody (ab234873)
Paraffin-embedded human testis tissue stained for ATP9A using ab234873 at 1/100 dilution in immunohistochemical anaysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP9A antibody (ab234873)
Paraffin-embedded human brain tissue stained for ATP9A using ab234873 at 1/100 dilution in immunohistochemical anaysis.
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