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AB234873

Anti-ATP9A antibody

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(1 Publication)

Rabbit Polyclonal ATP9A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP9A aa 1-100.

View Alternative Names

ATPIIA, KIAA0611, ATP9A, Probable phospholipid-transporting ATPase IIA, ATPase class II type 9A

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ATP9A antibody (AB234873)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP9A antibody (AB234873)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATP9A (green) using ab234873 at 1/100 dilution in ICC/IF. Secondary antibody was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP9A antibody (AB234873)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP9A antibody (AB234873)

Paraffin-embedded human brain tissue stained for ATP9A using ab234873 at 1/100 dilution in immunohistochemical anaysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP9A antibody (AB234873)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP9A antibody (AB234873)

Paraffin-embedded human testis tissue stained for ATP9A using ab234873 at 1/100 dilution in immunohistochemical anaysis.

Western blot - Anti-ATP9A antibody (AB234873)
  • WB

Supplier Data

Western blot - Anti-ATP9A antibody (AB234873)

All lanes:

Western blot - Anti-ATP9A antibody (ab234873) at 1/500 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 119 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ATP9A aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O75110

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP9A also known as ATPase phospholipid transporting 9A is a protein that acts as a phospholipid flippase involved in the movement of phospholipids across cellular membranes. This protein has a mass of approximately 125 kDa and is expressed in various tissues with notable presence in the brain and liver. ATP9A contains important structural domains that enable it to bind and hydrolyze ATP driving the translocation of phospholipids to maintain the asymmetry of lipid bilayers.
Biological function summary

The ATP9A protein plays an important role in membrane trafficking processes which are essential for proper cellular communication and cargo transport. It does not operate alone; ATP9A often associates with other cellular components to drive vesicular trafficking events. The protein is vital in endosomal sorting contributing to the correct targeting of proteins and lipids within cells. Dysregulation in its activity can disrupt these processes pointing to its importance in maintaining cellular homeostasis.

Pathways

The functions of ATP9A integrate into mechanisms like endocytosis and phospholipid metabolism. It has a connection with the phosphatidylinositol signalling pathway which is critical for vesicle transport and membrane dynamics. ATP9A interacts with proteins such as Rab11 a small GTPase involved in recycling endosomes reflecting its importance in endocytic recycling processes. These interconnected activities demonstrate ATP9A's role in regulating intracellular lipid distribution and movement.

ATP9A is particularly significant in oncological and neurological contexts. Alterations in its expression or function have been linked to cancer progression due to changes in cell migration and invasion linked to phospholipid dysregulation. Furthermore its involvement in proper neuromodulation in the brain connects ATP9A to neurodegenerative diseases. Relatedly ATP9A influences the activity of SNARE proteins important for synaptic vesicle fusion highlighting its broader impact on synaptic health and pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in regulating membrane trafficking of cargo proteins, namely endosome to plasma membrane recycling, probably acting through RAB5 and RAB11 activation (PubMed : 27733620, PubMed : 30213940, PubMed : 36604604). Also involved in endosome to trans-Golgi network retrograde transport (PubMed : 27733620, PubMed : 30213940). In complex with MON2 and DOP1B, regulates SNX3 retromer-mediated endosomal sorting of WLS, a transporter of Wnt morphogens in developing tissues. Participates in the formation of endosomal carriers that direct WLS trafficking back to Golgi, away from lysosomal degradation (PubMed : 30213940). Appears to be implicated in intercellular communication by negatively regulating the release of exosomes (PubMed : 30947313). The flippase activity towards membrane lipids and its role in membrane asymmetry remains to be proved (PubMed : 30947313). Required for the maintenance of neurite morphology and synaptic transmission (By similarity).
See full target information ATP9A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:855-869 PubMed36403197

2022

TTI1 promotes non-small-cell lung cancer progression by regulating the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Xian Zhang,Xin Yang,Zhi-Bo Wu,Zhong-Min Liao,Ding-Guo Wang,Shi-Wei Chen,Feng Lu,Yong-Bing Wu,Shu-Qiang Zhu
View all publications
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Product promise

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