AB101518

Anti-ATPAF1 antibody

Name: Anti-ATPAF1 antibody (ab101518) | Abcam
Brand: Abcam Limited
SKU: AB101518

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(3 Publications)

Rabbit Polyclonal ATPAF1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATPAF1 aa 100-250.

View Alternative Names

ATP11, ATPAF1, ATP synthase mitochondrial F1 complex assembly factor 1, ATP11 homolog

1 Images

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Western blot - Anti-ATPAF1 antibody (AB101518)

10% SDS-PAGE

All lanes:

Western blot - Anti-ATPAF1 antibody (ab101518) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 35 kDa,36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ATPAF1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q5TC12

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATPAF1 also known as ATP Synthase Mitochondrial F1 Complex Assembly Factor 1 functions as a mitochondrial chaperone assisting in the assembly of ATP synthase. This protein weighs approximately 35 kDa. Its expression occurs mainly in tissues with high energy demands such as the heart and skeletal muscle. ATPAF1 helps ensure proper M-ATPase complex formation essential for cellular ATP production.

Biological function summary

ATPAF1 supports mitochondrial energy metabolism by participating in assembling and stabilizing the ATP synthase complex also referred to as Complex V. Without ATPAF1 cells struggle to maintain efficient ATP synthesis due to improper complex assembly. It partners with other assembly factors such as ATPAF2 to facilitate the construction and proper function of this critical mitochondrial enzyme.

Pathways

ATPAF1 operates within the oxidative phosphorylation pathway serving an important role in ATP generation. This pathway drives most cellular processes by producing ATP through electron transport and chemiosmosis. ATPAF1 relates closely to the mitochondrial proteins involved in the electron transport chain particularly with ATP5F1 which forms part of ATP synthase's core structure.

ATPAF1 has links to mitochondrial diseases such as Leigh syndrome and mitochondrial myopathy. Patients with defects in ATPAF1 or related proteins may experience issues in energy-dependent tissues leading to symptoms like muscle weakness and neurological dysfunction. ATPAF1 interacts with other proteins like DNAJC19 which also associates with mitochondrial integrity and energy metabolism disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has a complex stabilizing activity in the assembly of the mitochondrial F1-F0 complex.

See full target information ATPAF1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Anais da Academia Brasileira de Ciencias 95:e20230480 PubMed38088732

2023

LINC00115 promotes gastric cancer partly by the miR-212-5p/ATPAF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxi Zhu,Jie Tan,Ting Zhan,Meng Liu,Yanli Zou,Weijie Liu

American journal of physiology. Endocrinology and 313:E335-E343 PubMed28465283

2017

Expression of microRNAs and target proteins in skeletal muscle of rats selectively bred for high and low running capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel K Pinto,Séverine Lamon,Erin J Stephenson,Ming Kalanon,Jasmine Mikovic,Lauren G Koch,Steven L Britton,John A Hawley,Donny M Camera

American journal of physiology. Endocrinology and 311:E836-E849 PubMed27677502

2016

Protein coingestion with alcohol following strenuous exercise attenuates alcohol-induced intramyocellular apoptosis and inhibition of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

William J Smiles,Evelyn B Parr,Vernon G Coffey,Orly Lacham-Kaplan,John A Hawley,Donny M Camera

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATPAF1 antibody

Western blot - Anti-ATPAF1 antibody (AB101518)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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