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AB74235

Anti-ATPAF2 antibody

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(1 Publication)

Rabbit Polyclonal ATPAF2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATPAF2.

View Alternative Names

ATP12, LP3663, ATPAF2, ATP synthase mitochondrial F1 complex assembly factor 2, ATP12 homolog

1 Images
Western blot - Anti-ATPAF2 antibody (AB74235)
  • WB

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Western blot - Anti-ATPAF2 antibody (AB74235)

All lanes:

Western blot - Anti-ATPAF2 antibody (ab74235) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 5 µg

Lane 2:

Extracts from Jurkat cells plus 5µg immunizing peptide at 5 µg

Predicted band size: 33 kDa

Observed band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ATPAF2. The exact immunogen used to generate this antibody is proprietary information.

Q8N5M1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ATPAF2 protein also known as ATP synthase mitochondrial F1 complex assembly factor 2 plays a significant role in the mitochondrial ATP synthase assembly. It is approximately 33 kDa in size. This protein is expressed mainly in tissues with high-energy demands such as muscle and brain tissue. It aids in the proper assembly of the ATP synthase complex by supporting the integration of certain subunits which is vital for maintaining mitochondrial function and energy production.
Biological function summary

ATPAF2 assists in the proper assembly of proton-translocating ATP synthase complexes within mitochondria. It is not directly a part of the ATP synthase complex but acts as an assembly factor that facilitates the inclusion of F1 complex components. This participation is important for the generation of ATP via oxidative phosphorylation—a process pivotal for cellular energy management.

Pathways

ATPAF2 is essential for the efficient functioning of the oxidative phosphorylation pathway. This pathway is responsible for ATP production in cells via the mitochondrial electron transport chain. Within this pathway ATPAF2 relates closely with ATP5B and ATP5C1 proteins which form integral parts of the ATP synthase complex. These interactions help ensure that ATP synthase is constructed correctly enabling the efficient synthesis of ATP.

ATPAF2 mutations have been associated with mitochondrial diseases and neurodegenerative disorders. Mitochondrial abnormalities caused by improper ATP synthase assembly can lead to conditions like Leigh syndrome. Proteins like ATP5A1 and ATP5O which also participate in the biogenesis of ATP synthase can demonstrate interconnected malfunction when ATPAF2 is impaired contributing to the severity of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the assembly of the F1 component of the mitochondrial ATP synthase (ATPase).
See full target information ATPAF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ systems biology and applications 11:67 PubMed40593928

2025

Temporal analysis of doxorubicin-induced cardiac toxicity and hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Te Lin,Yi-Ju Lee,Wen-Wei Tseng,Zih-Hua Chen,Huai-Ching Hsieh,Ko-Hong Lin,Jin-Yu Su,An-Chi Wei
View all publications
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Product promise

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