AB223779

Anti-ATPase Inhibitory Factor 1/IF1 antibody

Name: Anti-ATPase Inhibitory Factor 1/IF1 antibody (ab223779) | Abcam
Brand: Abcam Limited
SKU: AB223779

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(1 Publication)

Rabbit Polyclonal ATPase Inhibitory Factor 1/IF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP5IF1 aa 1-150.

View Alternative Names

ATPI, ATPIF1, ATP5IF1, ATP synthase F1 subunit epsilon, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ATPase Inhibitory Factor 1/IF1 (green) using ab223779 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human kidney tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human fallopian tube tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human duodenum tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human testis tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Paraffin-embedded human rectum tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

All lanes:

Western blot - Anti-ATPase Inhibitory Factor 1/IF1 antibody (ab223779) at 0.4 µg/mL

All lanes:

Human heart tissue

Predicted band size: 12 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ATP5IF1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9UII2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATPase Inhibitory Factor 1 (IF1) also referred to as IF1 protein ATPIF1 or ATPase inhibitory factor 1 is a small protein with a mass of approximately 12 kDa. It is expressed primarily in mitochondria-rich tissues such as the heart liver and brain where it plays an important role in energy metabolism. IF1 is notable for its ability to bind to the F1 subunit of the mitochondrial ATP synthase inhibiting its ATPase activity under specific conditions.

Biological function summary

IF1 influences mitochondrial function by regulating ATP synthase in response to changes in the cellular environment. Under conditions of low cellular energy IF1 stabilizes and prevents hydrolysis of ATP by binding to the F1 catalytic domain of the enzyme. This regulation helps to conserve ATP critical for maintaining cellular viability during stress. IF1 can also interact with other mitochondrial proteins to form complexes that influence energy homeostasis in the cell.

Pathways

IF1 integrates into pathways involved in cellular energy management including oxidative phosphorylation and ATP synthesis pathways. It interacts significantly with proteins such as the mitochondrial F1Fo-ATP synthase complex. By modulating ATP synthase activity IF1 ensures efficient ATP production and maintains mitochondrial membrane potential important for proper cell function.

IF1 has associations with metabolic conditions and cancer. Abnormal expression or activity of IF1 correlates with mitochondrial dysfunction often observed in cancer cells where it affects cell proliferation. Additionally IF1 has links to cardiac conditions given its role in regulating heart metabolism. It relates to proteins like the cytochrome c oxidase influencing cellular respiration and affecting disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix (PubMed : 35926043). Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues : regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.

See full target information ATP5IF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40954077

2025

LncRNA-CTD suppresses metastasis and immune evasion by modulating snail1 and MHC-I expression in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Xu,Huisi Qiu,Yuezhang Sun,Hong Jin,Siyu Yan,Fangmei Zhang,Ying Song,Guopei Zheng,Dongjiang Liao,Xiaoli Wei,Liyun Luo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATPase Inhibitory Factor 1/IF1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Western blot - Anti-ATPase Inhibitory Factor 1/IF1 antibody (AB223779)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reagents, Controls & Accessories

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise