AB14730

Anti-ATPB antibody [3D5] - Mitochondrial Marker

Name: Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) | Abcam
Brand: Abcam Limited
SKU: AB14730
Price: 400 USD
Availability: InStock
Rating: 5 (42 reviews)

Lab Essentials
What is this?

(42 Reviews)

(297 Publications)

Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) is a mouse monoclonal antibody detecting ATPB in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Caenorhabditis elegans, Cow, Human, Mouse, Rat.

- Over 250 publications
- Trusted since 2004

View Alternative Names

ATP5B, ATPMB, ATPSB, ATP5F1B, ATP synthase F1 subunit beta

7 Images

Lab

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

Merged signal (red and green). Green - ab14730 observed at 52 kDa. Red - loading control, ab181602 observed at 37 kDa.

Samples were subjected to SDS-PAGE. ab14730 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1ug/mL and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) (ab216773) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

Lane 1:

HeLa at 20 µg

Lane 2:

HepG2 at 20 µg

Lane 3:

Hu liver at 20 µg

Lane 4:

Ms liver at 20 µg

Predicted band size: 56 kDa

false

Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

Flow Cyt

Unknown

Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

ab14730 (blue) at 1μg/ml staining ATPB in HL-60 cells and analyzed by Flow cytometry. Red histogram represents equal quantity of isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

ab14730 staining ATPB in cultured human fibroblasts. Cells were fixed, permeabilized and then labelled with ab14730 followed by an AlexaFluor® 488-conjugated Goat anti-Mouse IgG1-specific secondary antibody (2 μg/ml)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

ab14730 (2µg/ml) staining ATPB in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and mitochondrial staining of epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

Immunohistochemical analysis of formalin fixed paraffin embedded human muscle labelling ATPB with ab14730 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins. ab14730 anti-ATPB antibody [3D5] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Unknown

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

All lanes:

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) at 0.8 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 56 kDa

Observed band size: 52 kDa

false

Unknown

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

All lanes:

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

Lane 1:

isolated mitochondria from human heart at 5 µg

Lane 2:

isolated mitochondria from bovine heart at 1 µg

Lane 3:

isolated mitochondria from rat heart at 10 µg

Lane 4:

isolated mitochondria from mouse heart at 10 µg

Predicted band size: 56 kDa

Observed band size: 52 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ATPB antibody [3D5] - Mitochondrial Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker
HRP

HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3D5

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human, Caenorhabditis elegans

Applications

Flow Cyt, WB, IHC-P, ICC/IF

applications

Specificity

Human and Bovine complex V beta subunit (ATPB).

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Reactivity data

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Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "" }, "Cat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Common marmoset": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Caenorhabditis elegans, Cow, Human, Mouse, Rat samples.

What is the molecular weight of ATPB?
Anti-ATPB [3D5] - Mitochondrial Marker (ab14730) specifically detects a band for ATPB (UniProt: P06576) at a molecular weight of 52kDa.

Trusted by the scientific community
Anti-ATPB [3D5] - Mitochondrial Marker (ab14730) was first used in a scientific publication in 2004 and has been cited over 250 times in peer-reviewed journals.

Reviewed by scientists
Anti-ATPB [3D5] - Mitochondrial Marker (ab14730) has over 40 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [3D5] also available for your convenience: ab14730, Alexa Fluor® 647 - ab197649, Alexa Fluor® 488 - ab197904, HRP - ab197905

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.4 - 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATPB also known as ATP synthase subunit beta is an essential protein component of the ATP synthase complex. It has an approximate mass of 52 kDa and is primarily expressed in the mitochondria. The protein's role is to catalyze the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate utilizing the proton gradient across the inner mitochondrial membrane. This process is central to the cellular energy production often referred to as oxidative phosphorylation. ATPB is frequently used as a mitochondrial marker in research making it an important target for antibodies such as those conjugated with Alexa Fluor 647 for immunofluorescence applications.

Biological function summary

ATPB functions as part of the mitochondrial ATP synthase complex which is also known as complex V of the electron transport chain. This complex is important for maintaining cellular energy homeostasis through ATP production. ATPB contributes to the catalytic activity necessary for ATP synthesis therefore supporting various cellular processes that require energy input such as muscle contraction and active transport. The protein also plays a role in coupling the proton motive force to ATP synthesis a function critical for mitochondrial efficiency and metabolic health.

Pathways

ATPB involves itself significantly in the oxidative phosphorylation and glycolysis pathways. It partners with other proteins in the ATP synthase complex such as ATP synthase subunit alpha (ATP5A1) to effectuate the conversion of energy. In the broader scope of energy metabolism ATPB integrates with glycolysis where glycolytic end-products feed into oxidative phosphorylation sustaining the cell’s energy currency. Both pathways are important for cells especially in tissues with high energy demands like the heart and skeletal muscles.

ATPB has been implicated in mitochondrial dysfunction-related diseases such as mitochondrial myopathy and Leigh syndrome. These conditions often result from mutations or defects in components of the electron transport chain leading to impaired ATP production. ATPB’s close connection to ATP5A1 and other complex V proteins highlights its involvement in these disorders. Understanding ATPB's role and function helps in disease mechanism elucidation and potentially offers targets for therapeutic interventions in mitochondrial-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

See full target information ATP5F1B

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Publications (297)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:188 PubMed40898372

2025

Optineurin deficiency disrupts phosphorylated tau proteostasis and clusterin expression in human neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary M Augur,Garrett M Fogo,Mason R Arbery,Andrew M Stern,Courtney R Benoit,Yi-Chen Hsieh,Tracy L Young-Pearse

Nature communications 16:7819 PubMed40841557

2025

Prefrontal cortex astrocytes modulate distinct neuronal populations to control anxiety-like behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Eunyoung Kim,Hairuo Du,Yanqi Tan,Brandon L Brown,Yaowen Chang,Blanca Díaz-Castro,Jonathan V Sweedler,Xinzhu Yu

Nature neuroscience 28:1844-1857 PubMed40790270

2025

Potentiation of mitochondrial function by mitoDREADD-G reverses pharmacological and neurodegenerative cognitive impairment in mice.

Applications

Species

Unspecified reactive species

Antonio C Pagano Zottola,Rebeca Martín-Jiménez,Gianluca Lavanco,Geneviève Hamel-Côté,Carla Ramon-Duaso,Rui S Rodrigues,Yamuna Mariani,Mehtab Khan,Filippo Drago,Stephanie Jean,Itziar Bonilla-Del Río,Daniel Jimenez-Blasco,Jon Egaña-Huguet,Abel Eraso-Pichot,Sandra Beriain,Astrid Cannich,Laura Vidal-Palencia,Rosmara Infantino,Francisca Julio-Kalajzić,Doriane Gisquet,Ania Goncalves,Inas Al-Younis,Yann Baussan,Stephane Duvezin-Caubet,Anne Devin,Edgar Soria-Gomez,Nagore Puente,Juan P Bolaños,Pedro Grandes,Sandrine Pouvreau,Arnau Busquets-Garcia,Giovanni Marsicano,Luigi Bellocchio,Etienne Hebert-Chatelain

Proceedings of the National Academy of Sciences of the United States of America 121:e2401996121 PubMed40591563

2025

UPF1 deficiency enhances mitochondrial ROS which promotes an immunosuppressive microenvironment in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Su,Juan Kochen Rossi,Cristina Nuevo-Tapioles,Ting Chen,Emily Kawaler,Cristina Branco,Kwok-Kin Wong,Diane M Simeone,Lawrence B Gardner,Mark R Philips

Nature communications 16:5149 PubMed40461502

2025

Sequential emergence and contraction of epithelial subtypes in the prenatal human choroid plexus revealed by a stem cell model.

Applications

Unspecified application

Species

Unspecified reactive species

Haley Masters,Shuxiong Wang,Christina Tu,Quy Nguyen,Yutong Sha,Matthew K Karikomi,Pamela Shi Ru Fung,Benjamin Tran,Cristina Martel,Nellie Kwang,Michael Neel,Olga G Jaime,Victoria Espericueta,Brett A Johnson,Kai Kessenbrock,Qing Nie,Edwin S Monuki

Nature communications 16:5133 PubMed40461459

2025

ROMO1 overexpression protects the mitochondrial cysteinome from oxidations in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Fengli Xu,Haipeng Huang,Kun Peng,Chongshu Jian,Hao Wu,Zhiwen Jing,Shan Qiu,Ying Chen,Keke Liu,Ling Fu,Yanru Wang,Jing Yang,Xiaotao Duan,Chu Wang,Heping Cheng,Xianhua Wang

Biology direct 20:65 PubMed40414862

2025

Macrophage Notch1 drives septic cardiac dysfunction by impairing mitophagy and promoting NLRP3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Zheng,Jingrong Lin,Guoqing Wan,Xuefeng Gu,Jian Ma

iScience 28:112390 PubMed40330889

2025

Agent-based modeling of neuronal mitochondrial dynamics using intrinsic variables of individual mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Garrett M Fogo,Francisco J Torres Torres,Reagan L Speas,Anthony R Anzell,Thomas H Sanderson

Journal of neuroinflammation 22:55 PubMed40022162

2025

Protective role of mitophagy on microglia-mediated neuroinflammatory injury through mtDNA-STING signaling in manganese-induced parkinsonism.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Lu,Liang Gao,Yuqing Yang,Dihang Shi,Zhipeng Zhang,Xiaobai Wang,Ying Huang,Jie Wu,Jia Meng,Hong Li,Dongying Yan

The Journal of clinical investigation 135: PubMed39724071

2024

Tagless LysoIP for immunoaffinity enrichment of native lysosomes from clinical samples.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Saarela,Pawel Lis,Sara Gomes,Raja S Nirujogi,Wentao Dong,Eshaan Rawat,Sophie Glendinning,Karolina Zeneviciute,Enrico Bagnoli,Rotimi Fasimoye,Cindy Lin,Kwamina Nyame,Fanni A Boros,Friederike Zunke,Frederic Lamoliatte,Sadik Elshani,Matthew Jaconelli,Judith Jm Jans,Margriet A Huisman,Christian Posern,Lena M Westermann,Angela Schulz,Peter M van Hasselt,Dario R Alessi,Monther Abu-Remaileh,Esther M Sammler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATPB antibody [3D5] - Mitochondrial Marker

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (297)

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