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Anti-ATPB antibody [3D5] ab14730 is a mouse monoclonal antibody that is used in ATPB western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Antibody clone 3D5 is the most widely used clone for ATPB on the market
- One antibody for all your ATPB staining


Images

Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730), expandable thumbnail
  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730), expandable thumbnail
  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4 - 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected
Caenorhabditis elegans
Expected
Expected
Expected
Expected
Cat
Predicted
Predicted
Predicted
Predicted
Common marmoset
Predicted
Predicted
Predicted
Predicted
Cow
Expected
Tested
Expected
Expected
Dog
Predicted
Predicted
Predicted
Predicted
Goat
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Species
Cow
Dilution info
-
Notes

-

Species
Caenorhabditis elegans
Dilution info
1 µg/mL
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Pig, Common marmoset, Cat, Dog, Goat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.5 µg/mL
Notes

-

Species
Rat
Dilution info
0.5 µg/mL
Notes

-

Species
Human
Dilution info
0.5 µg/mL
Notes

-

Species
Cow
Dilution info
0.5 µg/mL
Notes

-

Expected
Expected

Species
Caenorhabditis elegans
Dilution info
0.5 µg/mL
Notes

-

Predicted
Predicted

Species
Pig, Common marmoset, Cat, Dog, Goat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Cow, Caenorhabditis elegans
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Pig, Common marmoset, Cat, Dog, Goat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Species
Cow
Dilution info
-
Notes

-

Species
Caenorhabditis elegans
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Predicted
Predicted

Species
Pig, Common marmoset, Cat, Dog, Goat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Alternative names

ATP5B, ATPMB, ATPSB, ATP5F1B, ATP synthase F1 subunit beta

Recommended products

Anti-ATPB antibody [3D5] ab14730 is a mouse monoclonal antibody that is used in ATPB western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Antibody clone 3D5 is the most widely used clone for ATPB on the market
- One antibody for all your ATPB staining

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Clone number
3D5
Purity
IgG fraction
Specificity

Human and Bovine complex V beta subunit (ATPB).

Light chain type
kappa
Concentration
Loading...
Purification notes

Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATPB also known as ATP synthase subunit beta is an essential protein component of the ATP synthase complex. It has an approximate mass of 52 kDa and is primarily expressed in the mitochondria. The protein's role is to catalyze the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate utilizing the proton gradient across the inner mitochondrial membrane. This process is central to the cellular energy production often referred to as oxidative phosphorylation. ATPB is frequently used as a mitochondrial marker in research making it an important target for antibodies such as those conjugated with Alexa Fluor 647 for immunofluorescence applications.

Biological function summary

ATPB functions as part of the mitochondrial ATP synthase complex which is also known as complex V of the electron transport chain. This complex is important for maintaining cellular energy homeostasis through ATP production. ATPB contributes to the catalytic activity necessary for ATP synthesis therefore supporting various cellular processes that require energy input such as muscle contraction and active transport. The protein also plays a role in coupling the proton motive force to ATP synthesis a function critical for mitochondrial efficiency and metabolic health.

Pathways

ATPB involves itself significantly in the oxidative phosphorylation and glycolysis pathways. It partners with other proteins in the ATP synthase complex such as ATP synthase subunit alpha (ATP5A1) to effectuate the conversion of energy. In the broader scope of energy metabolism ATPB integrates with glycolysis where glycolytic end-products feed into oxidative phosphorylation sustaining the cell’s energy currency. Both pathways are important for cells especially in tissues with high energy demands like the heart and skeletal muscles.

Associated diseases and disorders

ATPB has been implicated in mitochondrial dysfunction-related diseases such as mitochondrial myopathy and Leigh syndrome. These conditions often result from mutations or defects in components of the electron transport chain leading to impaired ATP production. ATPB’s close connection to ATP5A1 and other complex V proteins highlights its involvement in these disorders. Understanding ATPB's role and function helps in disease mechanism elucidation and potentially offers targets for therapeutic interventions in mitochondrial-related diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730), expandable thumbnail

    Flow Cytometry - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    ab14730 (blue) at 1μg/ml staining ATPB in HL-60 cells and analyzed by Flow cytometry. Red histogram represents equal quantity of isotype control.

  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730), expandable thumbnail

    Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    Merged signal (red and green). Green - ab14730 observed at 52 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa.

    Samples were subjected to SDS-PAGE. ab14730 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1ug/mL and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    Lane 1: HeLa at 20 µg

    Lane 2: HepG2 at 20 µg

    Lane 3: Hu liver at 20 µg

    Lane 4: Ms liver at 20 µg

    Performed under reducing conditions.

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    ab14730 staining ATPB in cultured human fibroblasts. Cells were fixed, permeabilized and then labelled with ab14730 followed by an AlexaFluor® 488-conjugated Goat anti-Mouse IgG1-specific secondary antibody (2 μg/ml)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    ab14730 (2µg/ml) staining ATPB in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and mitochondrial staining of epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730), expandable thumbnail

    Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    All lanes: Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    Lane 1: isolated mitochondria from human heart at 5 µg

    Lane 2: isolated mitochondria from bovine heart at 1 µg

    Lane 3: isolated mitochondria from rat heart at 10 µg

    Lane 4: isolated mitochondria from mouse heart at 10 µg

    Predicted band size: 56 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730), expandable thumbnail

    Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)

    All lanes: Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) at 0.8 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 3: Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 56 kDa

    Observed band size: 52 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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