Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (AB5432)

ICC/IF image of ab5432 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (AB5432)

Immunofluorescent analysis of ATPB in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1 : 200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (AB5432)

Immunocytochemistry/Immunofluorescent analysis of ATPB (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with ab5432 at 1 : 100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1 : 200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (AB5432)

Immunofluorescent analysis of ATPB in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATPB monoclonal antibody (ab5432) at a dilution of 1 : 200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. ATPB staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (AB5432)

Immunocytochemistry analysis of ATPB using ab5432 at 5μg/mL concentration shows staining in 4% paraformaldehyde-fixed 3T3 Cells. Secondary was Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at 1/1000 dilution. ATPB (green), and nuclei with Hoechst 33342 dye (blue) is shown. Negative control has no primary antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (AB5432)

Immunocytochemistry/Immunofluorescence analysis of rat neuronal/glial cell culture using ab5432.

• IP

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Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (AB5432)

ATPB was immunoprecipitated from THP-1 whole cell lysate with 5 μL ab5432.

Lane 1 : ab5432 IP in THP-1 whole cell lysate, with HRP-conjugated goat anti-mouse IgG secondary

Detection : Chemiluminescence

All lanes:

Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

Predicted band size: 56 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (AB5432)

Immunoprecipatation of rat neuronal/glial cell extract using ab5432.

All lanes:

Immunoprecipitation - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432)

Predicted band size: 56 kDa

false

• WB

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Western blot - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (AB5432)

All lanes:

Western blot - Anti-ATPB antibody [4.3E8.D10] - Mitochondrial Marker (ab5432) at 1 µg/mL

All lanes:

Human testis tissue lysate - total protein (<a href='/en-us/products/unavailable/human-testis-tissue-lysate-total-protein-ab30257'>ab30257</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 56 kDa

Observed band size: 57 kDa

true

Exposure time: 16min