Mouse Monoclonal ATPB antibody. Suitable for WB and reacts with Mouse, Rat, Cow, Human samples. Cited in 9 publications.
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Rat | Tested |
Cow | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 4 µg/mL | Notes - |
Species Rat | Dilution info 4 µg/mL | Notes - |
Species Cow | Dilution info 4 µg/mL | Notes - |
Species Human | Dilution info 4 µg/mL | Notes - |
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Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
ATP5B, ATPMB, ATPSB, ATP5F1B, ATP synthase F1 subunit beta
Mouse Monoclonal ATPB antibody. Suitable for WB and reacts with Mouse, Rat, Cow, Human samples. Cited in 9 publications.
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
The purity of ab110280 is near homogeneity, as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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ATPB also known as ATP synthase subunit beta is an essential protein component of the ATP synthase complex. It has an approximate mass of 52 kDa and is primarily expressed in the mitochondria. The protein's role is to catalyze the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate utilizing the proton gradient across the inner mitochondrial membrane. This process is central to the cellular energy production often referred to as oxidative phosphorylation. ATPB is frequently used as a mitochondrial marker in research making it an important target for antibodies such as those conjugated with Alexa Fluor 647 for immunofluorescence applications.
ATPB functions as part of the mitochondrial ATP synthase complex which is also known as complex V of the electron transport chain. This complex is important for maintaining cellular energy homeostasis through ATP production. ATPB contributes to the catalytic activity necessary for ATP synthesis therefore supporting various cellular processes that require energy input such as muscle contraction and active transport. The protein also plays a role in coupling the proton motive force to ATP synthesis a function critical for mitochondrial efficiency and metabolic health.
ATPB involves itself significantly in the oxidative phosphorylation and glycolysis pathways. It partners with other proteins in the ATP synthase complex such as ATP synthase subunit alpha (ATP5A1) to effectuate the conversion of energy. In the broader scope of energy metabolism ATPB integrates with glycolysis where glycolytic end-products feed into oxidative phosphorylation sustaining the cell’s energy currency. Both pathways are important for cells especially in tissues with high energy demands like the heart and skeletal muscles.
ATPB has been implicated in mitochondrial dysfunction-related diseases such as mitochondrial myopathy and Leigh syndrome. These conditions often result from mutations or defects in components of the electron transport chain leading to impaired ATP production. ATPB’s close connection to ATP5A1 and other complex V proteins highlights its involvement in these disorders. Understanding ATPB's role and function helps in disease mechanism elucidation and potentially offers targets for therapeutic interventions in mitochondrial-related diseases.
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ATPB Western blot staining using mouse Anti-ATPB antibody
All lanes: Western blot - Anti-ATPB antibody [7E3F2] - Mitochondrial Marker (ab110280) at 4 µg/mL
Lane 1: Isolated mitochondria from Human heart at 15 µg
Lane 2: Isolated mitochondria from cow heart at 6 µg
Lane 3: Isolated mitochondria from rat heart at 30 µg
Lane 4: Isolated mitochondria from mouse heart at 30 µg
Lane 5: Isolated mitochondria from HepG2 cells at 30 µg
Predicted band size: 56 kDa
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