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AB110280

Anti-ATPB antibody [7E3F2] - Mitochondrial Marker

5

(1 Review)

|

(9 Publications)

Mouse Monoclonal ATPB antibody. Suitable for WB and reacts with Mouse, Rat, Cow, Human samples. Cited in 9 publications.

View Alternative Names

ATP5B, ATPMB, ATPSB, ATP5F1B, ATP synthase F1 subunit beta

1 Images
Western blot - Anti-ATPB antibody [7E3F2] - Mitochondrial Marker (AB110280)
  • WB

Unknown

Western blot - Anti-ATPB antibody [7E3F2] - Mitochondrial Marker (AB110280)

All lanes:

Western blot - Anti-ATPB antibody [7E3F2] - Mitochondrial Marker (ab110280) at 4 µg/mL

Lane 1:

Isolated mitochondria from Human heart at 15 µg

Lane 2:

Isolated mitochondria from cow heart at 6 µg

Lane 3:

Isolated mitochondria from rat heart at 30 µg

Lane 4:

Isolated mitochondria from mouse heart at 30 µg

Lane 5:

Isolated mitochondria from HepG2 cells at 30 µg

Predicted band size: 56 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7E3F2

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Cow, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
The purity of ab110280 is near homogeneity, as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATPB also known as ATP synthase subunit beta is an essential protein component of the ATP synthase complex. It has an approximate mass of 52 kDa and is primarily expressed in the mitochondria. The protein's role is to catalyze the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate utilizing the proton gradient across the inner mitochondrial membrane. This process is central to the cellular energy production often referred to as oxidative phosphorylation. ATPB is frequently used as a mitochondrial marker in research making it an important target for antibodies such as those conjugated with Alexa Fluor 647 for immunofluorescence applications.
Biological function summary

ATPB functions as part of the mitochondrial ATP synthase complex which is also known as complex V of the electron transport chain. This complex is important for maintaining cellular energy homeostasis through ATP production. ATPB contributes to the catalytic activity necessary for ATP synthesis therefore supporting various cellular processes that require energy input such as muscle contraction and active transport. The protein also plays a role in coupling the proton motive force to ATP synthesis a function critical for mitochondrial efficiency and metabolic health.

Pathways

ATPB involves itself significantly in the oxidative phosphorylation and glycolysis pathways. It partners with other proteins in the ATP synthase complex such as ATP synthase subunit alpha (ATP5A1) to effectuate the conversion of energy. In the broader scope of energy metabolism ATPB integrates with glycolysis where glycolytic end-products feed into oxidative phosphorylation sustaining the cell's energy currency. Both pathways are important for cells especially in tissues with high energy demands like the heart and skeletal muscles.

ATPB has been implicated in mitochondrial dysfunction-related diseases such as mitochondrial myopathy and Leigh syndrome. These conditions often result from mutations or defects in components of the electron transport chain leading to impaired ATP production. ATPB's close connection to ATP5A1 and other complex V proteins highlights its involvement in these disorders. Understanding ATPB's role and function helps in disease mechanism elucidation and potentially offers targets for therapeutic interventions in mitochondrial-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit beta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable) (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the subunit alpha (ATP5F1A), forms the catalytic core in the F(1) domain (PubMed : 37244256).
See full target information ATP5F1B
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 19:e1010776 PubMed37871041

2023

A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical in Sinorhizobium meliloti for symbiosis with legume hosts.

Applications

Unspecified application

Species

Unspecified reactive species

Julian A Bustamante,Josue S Ceron,Ivan Thomas Gao,Hector A Ramirez,Milo V Aviles,Demsin Bet Adam,Jason R Brice,Rodrigo A Cuellar,Eva Dockery,Miguel Karlo Jabagat,Donna Grace Karp,Joseph Kin-On Lau,Suling Li,Raymondo Lopez-Magaña,Rebecca R Moore,Bethany Kristi R Morin,Juliana Nzongo,Yasha Rezaeihaghighi,Joseph Sapienza-Martinez,Tuyet Thi Kim Tran,Zhenzhong Huang,Aaron J Duthoy,Melanie J Barnett,Sharon R Long,Joseph C Chen

Journal of bacteriology 203:e0014321 PubMed33941609

2021

Low Cytoplasmic Magnesium Increases the Specificity of the Lon and ClpAP Proteases.

Applications

Unspecified application

Species

Unspecified reactive species

Jinki Yeom,Eduardo A Groisman

Science signaling 14: PubMed33500334

2021

Reduced ATP-dependent proteolysis of functional proteins during nutrient limitation speeds the return of microbes to a growth state.

Applications

Unspecified application

Species

Unspecified reactive species

Jinki Yeom,Eduardo A Groisman

Proceedings of the National Academy of Sciences of 117:20235-20243 PubMed32753384

2020

Small proteins regulate survival inside macrophages by controlling degradation of a magnesium transporter.

Applications

Unspecified application

Species

Unspecified reactive species

Jinki Yeom,Yi Shao,Eduardo A Groisman

Advanced science (Weinheim, Baden-Wurttemberg, Ger 6:1901673 PubMed31763149

2019

Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Eol Park,Dongkil Choi,Ji Su Park,Changgon Sim,Sohyun Park,Sunah Kang,Hyunsoo Yim,Myungsun Lee,Jaeyoun Kim,Jinyoung Pac,Kunsoo Rhee,Junho Lee,Yunjong Lee,Yan Lee,Sung-Yon Kim

Chemosphere 222:722-731 PubMed30738315

2019

Testosterone disruptor effect and gut microbiome perturbation in mice: Early life exposure to doxycycline.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Hou,Lei Zhu,Xianwei Zhang,Lili Zhang,Hongduo Bao,Minmin Tang,Ruicheng Wei,Ran Wang

Molecular cell 66:234-246.e5 PubMed28431231

2017

Sequestration from Protease Adaptor Confers Differential Stability to Protease Substrate.

Applications

Unspecified application

Species

Unspecified reactive species

Jinki Yeom,Kyle J Wayne,Eduardo A Groisman

Blood 120:1246-53 PubMed22753871

2012

Ectopic ATP synthase facilitates transfer of HIV-1 from antigen-presenting cells to CD4(+) target cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amichai Yavlovich,Mathias Viard,Ming Zhou,Timothy D Veenstra,Ji Ming Wang,Wanghua Gong,Eliahu Heldman,Robert Blumenthal,Yossef Raviv

BMB reports 41:153-7 PubMed18315952

2008

Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria.

Applications

WB

Species

Rat

Misun Kim,Jinsun Kim,Choong-Ill Cheon,Dae Ho Cho,Jong Hoon Park,Keun Il Kim,Kyo-Young Lee,Eunsook Song
View all publications
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Product promise

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