Skip to main content

Rabbit Recombinant Monoclonal ATPB antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 10 publications.


Images

Western blot - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (AB170947), expandable thumbnail
  • Immunoprecipitation - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Predicted
Predicted
Not recommended
Expected
Predicted
Rat
Predicted
Predicted
Not recommended
Expected
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Antigen retrieval is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

For unpurified use at 1/50 - 1/100.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Alternative names

ATP5B, ATPMB, ATPSB, ATP5F1B, ATP synthase F1 subunit beta

Recommended products

Rabbit Recombinant Monoclonal ATPB antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR11990
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATPB also known as ATP synthase subunit beta is an essential protein component of the ATP synthase complex. It has an approximate mass of 52 kDa and is primarily expressed in the mitochondria. The protein's role is to catalyze the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate utilizing the proton gradient across the inner mitochondrial membrane. This process is central to the cellular energy production often referred to as oxidative phosphorylation. ATPB is frequently used as a mitochondrial marker in research making it an important target for antibodies such as those conjugated with Alexa Fluor 647 for immunofluorescence applications.

Biological function summary

ATPB functions as part of the mitochondrial ATP synthase complex which is also known as complex V of the electron transport chain. This complex is important for maintaining cellular energy homeostasis through ATP production. ATPB contributes to the catalytic activity necessary for ATP synthesis therefore supporting various cellular processes that require energy input such as muscle contraction and active transport. The protein also plays a role in coupling the proton motive force to ATP synthesis a function critical for mitochondrial efficiency and metabolic health.

Pathways

ATPB involves itself significantly in the oxidative phosphorylation and glycolysis pathways. It partners with other proteins in the ATP synthase complex such as ATP synthase subunit alpha (ATP5A1) to effectuate the conversion of energy. In the broader scope of energy metabolism ATPB integrates with glycolysis where glycolytic end-products feed into oxidative phosphorylation sustaining the cell’s energy currency. Both pathways are important for cells especially in tissues with high energy demands like the heart and skeletal muscles.

Associated diseases and disorders

ATPB has been implicated in mitochondrial dysfunction-related diseases such as mitochondrial myopathy and Leigh syndrome. These conditions often result from mutations or defects in components of the electron transport chain leading to impaired ATP production. ATPB’s close connection to ATP5A1 and other complex V proteins highlights its involvement in these disorders. Understanding ATPB's role and function helps in disease mechanism elucidation and potentially offers targets for therapeutic interventions in mitochondrial-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947), expandable thumbnail

    Western blot - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947)

    ATPB Western blot staining using rabbit Anti-ATPB antibody

    All lanes: Western blot - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947) at 1/10000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: HT29 cell lysate at 10 µg

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947)

    ATPB Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-ATPB antibody

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling ATPB with purified ab170947 at 1/500 dilution. Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947)

    ATPB Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ATPB antibody

    ab170947 showing +ve staining in Human normal kidney tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)

    ab170947 showing +ve staining in Human normal uterus tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947), expandable thumbnail

    Immunoprecipitation - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947)

    All lanes: Immunoprecipitation - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947) at 1/10 dilution

    All lanes: HepG2 cell lysate

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    Predicted band size: 22 kDa, 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATPB with ab170947 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ATPB with ab170947 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947)

    ATPB Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-ATPB antibody

    Immunofluorescent analysis of HeLa cells labeling ATPB using ab170947 at 1/50 dilution (green). DAPI nuclear staining (blue).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com