AB170947

Anti-ATPB antibody [EPR11990] - Mitochondrial Marker

RabMAb
Recombinant
What is this?

(1 Review)

(12 Publications)

Rabbit Recombinant Monoclonal ATPB antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

View Alternative Names

ATP5B, ATPMB, ATPSB, ATP5F1B, ATP synthase F1 subunit beta

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ATPB with ab170947 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATPB with ab170947 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

ab170947 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling ATPB with purified ab170947 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunofluorescent analysis of HeLa cells labeling ATPB using ab170947 at 1/50 dilution (green). DAPI nuclear staining (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

ab170947 showing +ve staining in Human normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

All lanes:

Immunoprecipitation - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947) at 1/10 dilution

All lanes:

HepG2 cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Predicted band size: 22 kDa,56 kDa

false

Supplier Data

Western blot - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

All lanes:

Western blot - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (ab170947) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HT29 cell lysate at 10 µg

Predicted band size: 56 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ATPB antibody [EPR11990] - Mitochondrial Marker
Carrier free

Anti-ATPB antibody [EPR11990] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ATPB antibody [EPR11990] - Mitochondrial Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11990

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Antigen retrieval is recommended.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "For unpurified use at 1/50 - 1/100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATPB also known as ATP synthase subunit beta is an essential protein component of the ATP synthase complex. It has an approximate mass of 52 kDa and is primarily expressed in the mitochondria. The protein's role is to catalyze the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate utilizing the proton gradient across the inner mitochondrial membrane. This process is central to the cellular energy production often referred to as oxidative phosphorylation. ATPB is frequently used as a mitochondrial marker in research making it an important target for antibodies such as those conjugated with Alexa Fluor 647 for immunofluorescence applications.

Biological function summary

ATPB functions as part of the mitochondrial ATP synthase complex which is also known as complex V of the electron transport chain. This complex is important for maintaining cellular energy homeostasis through ATP production. ATPB contributes to the catalytic activity necessary for ATP synthesis therefore supporting various cellular processes that require energy input such as muscle contraction and active transport. The protein also plays a role in coupling the proton motive force to ATP synthesis a function critical for mitochondrial efficiency and metabolic health.

Pathways

ATPB involves itself significantly in the oxidative phosphorylation and glycolysis pathways. It partners with other proteins in the ATP synthase complex such as ATP synthase subunit alpha (ATP5A1) to effectuate the conversion of energy. In the broader scope of energy metabolism ATPB integrates with glycolysis where glycolytic end-products feed into oxidative phosphorylation sustaining the cell’s energy currency. Both pathways are important for cells especially in tissues with high energy demands like the heart and skeletal muscles.

ATPB has been implicated in mitochondrial dysfunction-related diseases such as mitochondrial myopathy and Leigh syndrome. These conditions often result from mutations or defects in components of the electron transport chain leading to impaired ATP production. ATPB’s close connection to ATP5A1 and other complex V proteins highlights its involvement in these disorders. Understanding ATPB's role and function helps in disease mechanism elucidation and potentially offers targets for therapeutic interventions in mitochondrial-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit beta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable) (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the subunit alpha (ATP5F1A), forms the catalytic core in the F(1) domain (PubMed : 37244256).

See full target information ATP5F1B

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Biology methods & protocols 10:bpaf007 PubMed39925781

2025

An improved cell nuclear isolation method.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Li,Jingyao Zhang,Xiaojuan Liu,Zhijuan Wu,Y James Kang,Wenjing Zhang

CNS neuroscience & therapeutics 30:e14377 PubMed37622283

2023

PGAM5 knockout causes depressive-like behaviors in mice via ATP deficiency in the prefrontal cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Cui,Chunhui Chen,Liya Gong,Junyan Wen,Shanshan Yang,Min Zheng,Baogui Gao,Junxiong You,Xuecong Lin,Yanyu Hao,Zhimin Chen,Ziqi Wu,Liaoming Gao,Jiayu Tang,Zhen Yuan,Xuegang Sun,Linlin Jing,Ge Wen

Autophagy 19:805-821 PubMed35920704

2022

TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tai-Mei Zhang,Li Liao,Shao-Ying Yang,Min-Ying Huang,Yin-Ling Zhang,Ling Deng,Shu-Yuan Hu,Fan Yang,Fang-Lin Zhang,Zhi-Min Shao,Da-Qiang Li

Frontiers in pharmacology 12:650983 PubMed34054531

2021

Guhong Injection Protects Against Apoptosis in Cerebral Ischemia by Maintaining Cerebral Microvasculature and Mitochondrial Integrity Through the PI3K/AKT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huifen Zhou,Yu He,Jiaqi Zhu,Xiaojie Lin,Juan Chen,Chongyu Shao,Haitong Wan,Jiehong Yang

Journal of neurochemistry 157:550-560 PubMed33305362

2020

Nicotinamide ameliorates energy deficiency and improves retinal function in Cav-1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhen Tang,Wangyi Fang,Zebin Xiao,Maomao Song,Dongli Zhuang,Binze Han,Jihong Wu,Xinghuai Sun

Journal of cellular and molecular medicine : PubMed32954626

2020

Involvement of kindlin-2 in irisin's protection against ischaemia reperfusion-induced liver injury in high-fat diet-fed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhang,Yifan Ren,Jianbin Bi,Mengzhou Wang,Lin Zhang,Tao Wang,Shasha Wei,Xingyi Mou,Yi Lv,Rongqian Wu

Journal of experimental & clinical cancer research 39:144 PubMed32727517

2020

IFI6 depletion inhibits esophageal squamous cell carcinoma progression through reactive oxygen species accumulation via mitochondrial dysfunction and endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenchuan Liu,Shaorui Gu,Tiancheng Lu,Kaiqing Wu,Lei Li,Chenglai Dong,Yongxin Zhou

Life sciences 256:117971 PubMed32553925

2020

SR18292 exerts potent antitumor effects in multiple myeloma via inhibition of oxidative phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xiang,Bin Fang,Yilin Liu,Siqi Yan,Dedong Cao,Huiling Mei,Qiuguo Wang,Yu Hu,Tao Guo

Breast cancer research and treatment 176:271-289 PubMed31006104

2019

A novel RNA aptamer identifies plasma membrane ATP synthase beta subunit as an early marker and therapeutic target in aggressive cancer.

Applications

Unspecified application

Species

Unspecified reactive species

S Speransky,P Serafini,J Caroli,S Bicciato,M E Lippman,N H Bishopric

Biometals : an international journal on the role o 32:329-341 PubMed30739301

2019

Profiling of nuclear copper-binding proteins under hypoxic condition.

Applications

Unspecified application

Species

Unspecified reactive species

Haiying Fu,Xueqin Ding,Wenjing Zhang,Y James Kang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATPB antibody [EPR11990] - Mitochondrial Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Immunoprecipitation - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

Western blot - Anti-ATPB antibody [EPR11990] - Mitochondrial Marker (AB170947)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise