Rabbit Polyclonal ATPBD3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CTU1 aa 50-200.
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Publications
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- Nature communications 10:54922019Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunwang Xu et. al.PubMed 31792210
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CTU1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z7A3
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
Alternative names
ATPBD3, NCS6, CTU1, Cytoplasmic tRNA 2-thiolation protein 1, ATP-binding domain-containing protein 3, Cancer-associated gene protein, Cytoplasmic tRNA adenylyltransferase 1
Recommended products
Rabbit Polyclonal ATPBD3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CTU1 aa 50-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CTU1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z7A3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATPBD3 also known as ATP-binding domain 3 functions as an enzyme involved in energy metabolism. It possesses ATPase activity which allows it to hydrolyze ATP the energy currency of the cell. With a molecular mass of approximately 60 kDa ATPBD3 plays a significant role in cellular energy management. This protein is widely expressed in numerous tissues indicating its importance in diverse cellular activities. Cells harness ATPBD3's energy-converting abilities to sustain various metabolic processes critical for cell viability and function.
- Biological function summary
ATPBD3 facilitates energy homeostasis and cellular metabolic processes. It performs as part of a complex that coordinates multiple cellular activities ensuring efficient energy production and utilization. This precise management of cellular energy resources is important for supporting cell survival promoting cell growth and regulating apoptosis. Through these activities ATPBD3 contributes significantly to maintaining cellular homeostasis and responding to metabolic demands.
- Pathways
ATPBD3 integrates into cellular energy metabolism and stress response pathways. It engages in ATP synthesis and hydrolysis processes critical for energy production and cellular adaptation. The protein functions alongside other metabolic enzymes like AMP-activated protein kinase (AMPK) which senses cellular energy status and regulates metabolic pathways. ATPBD3's role in these pathways showcases its involvement in cellular responses to energy fluctuations aiding cells in adapting to metabolic shifts.
- Associated diseases and disorders
Faulty regulation or expression of ATPBD3 associates with metabolic disorders such as obesity and type 2 diabetes. Abnormalities in ATPBD3 function can disrupt energy balance leading to metabolic imbalances linked to these conditions. Additionally ATPBD3 interactions with proteins such as AMPK highlight its potential influence on glucose metabolism and insulin signaling pathways which are dysregulated in metabolic diseases. Investigating ATPBD3's role and interactions may offer insights into novel therapeutic strategies for addressing these metabolic challenges.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-ATPBD3 antibody (ab185473)
Immunofluorescence analysis of Human PC3 cells labeling ATPBD3 with ab185473 at 4μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPBD3 antibody (ab185473)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling ATPBD3 with ab185473 at a 1/200 dilution.
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