AB185473

Anti-ATPBD3 antibody

Name: Anti-ATPBD3 antibody (ab185473) | Abcam
Brand: Abcam Limited
SKU: AB185473

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(2 Publications)

Rabbit Polyclonal ATPBD3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CTU1 aa 50-200.

View Alternative Names

ATPBD3, NCS6, CTU1, Cytoplasmic tRNA 2-thiolation protein 1, ATP-binding domain-containing protein 3, Cancer-associated gene protein, Cytoplasmic tRNA adenylyltransferase 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATPBD3 antibody (AB185473)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATPBD3 antibody (AB185473)

Immunofluorescence analysis of Human PC3 cells labeling ATPBD3 with ab185473 at 4μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPBD3 antibody (AB185473)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPBD3 antibody (AB185473)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling ATPBD3 with ab185473 at a 1/200 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CTU1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q7Z7A3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATPBD3 also known as ATP-binding domain 3 functions as an enzyme involved in energy metabolism. It possesses ATPase activity which allows it to hydrolyze ATP the energy currency of the cell. With a molecular mass of approximately 60 kDa ATPBD3 plays a significant role in cellular energy management. This protein is widely expressed in numerous tissues indicating its importance in diverse cellular activities. Cells harness ATPBD3's energy-converting abilities to sustain various metabolic processes critical for cell viability and function.

Biological function summary

ATPBD3 facilitates energy homeostasis and cellular metabolic processes. It performs as part of a complex that coordinates multiple cellular activities ensuring efficient energy production and utilization. This precise management of cellular energy resources is important for supporting cell survival promoting cell growth and regulating apoptosis. Through these activities ATPBD3 contributes significantly to maintaining cellular homeostasis and responding to metabolic demands.

Pathways

ATPBD3 integrates into cellular energy metabolism and stress response pathways. It engages in ATP synthesis and hydrolysis processes critical for energy production and cellular adaptation. The protein functions alongside other metabolic enzymes like AMP-activated protein kinase (AMPK) which senses cellular energy status and regulates metabolic pathways. ATPBD3's role in these pathways showcases its involvement in cellular responses to energy fluctuations aiding cells in adapting to metabolic shifts.

Faulty regulation or expression of ATPBD3 associates with metabolic disorders such as obesity and type 2 diabetes. Abnormalities in ATPBD3 function can disrupt energy balance leading to metabolic imbalances linked to these conditions. Additionally ATPBD3 interactions with proteins such as AMPK highlight its potential influence on glucose metabolism and insulin signaling pathways which are dysregulated in metabolic diseases. Investigating ATPBD3's role and interactions may offer insights into novel therapeutic strategies for addressing these metabolic challenges.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.

See full target information CTU1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:5492 PubMed31792210

2019

Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sunwang Xu,Ming Zhan,Cen Jiang,Min He,Linhua Yang,Hui Shen,Shuai Huang,Xince Huang,Ruirong Lin,Yongheng Shi,Qiang Liu,Wei Chen,Man Mohan,Jian Wang

Oncogene 37:722-731 PubMed29059175

2017

Induction of anti-VEGF therapy resistance by upregulated expression of microseminoprotein (MSMP).

Applications

Unspecified application

Species

Unspecified reactive species

T Mitamura,S Pradeep,M McGuire,S Y Wu,S Ma,H Hatakeyama,Y A Lyons,T Hisamatsu,K Noh,A Villar-Prados,X Chen,C Ivan,C Rodriguez-Aguayo,W Hu,G Lopez-Berestein,R L Coleman,A K Sood

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATPBD3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ATPBD3 antibody (AB185473)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPBD3 antibody (AB185473)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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