AB184137

Anti-ATR antibody

Name: Anti-ATR antibody (ab184137) | Abcam
Brand: Abcam Limited
SKU: AB184137
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ATR antibody. Suitable for WB, ICC/IF, IHC-P, IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ATR aa 1900 to C-terminus.

View Alternative Names

FRP1, ATR, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATR antibody (AB184137)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATR antibody (AB184137)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling ATR protein at nucleolus using ab184137 at a 1/5000 dilution (Green). Phalloidin, a cytoskeleton marker stained by anti-phalloidin antibody at a 1/200 dilution (Red). Hoechst 33342 was used for nuclear staining. Scale bar = 10 µm

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR antibody (AB184137)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR antibody (AB184137)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue stained for ATR protein at nucleus on human lung carcinoma with ab184137 at a 1/250 dilution.

Antigen Retrieval : EDTA based, pH 8.0 buffer, 15min.

Supplier Data

Immunoprecipitation - Anti-ATR antibody (AB184137)

Immunoprecipitation of ATR protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 µg of ATR antibody.
Western blot analysis was performed using ATR antibody.

All lanes:

Immunoprecipitation - Anti-ATR antibody (ab184137) at 5 µg

Lane 1:

HCT-116 whole cell extracts (Input)

Lane 2:

HCT-116 whole cell extracts with control IgG

Lane 3:

HCT-116 whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG Antibody

Observed band size: 90 kDa

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Supplier Data

Western blot - Anti-ATR antibody (AB184137)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ATR antibody (ab184137) at 1/500 dilution

Lane 1:

Raji whole cell extracts at 30 µg

Lane 2:

Raji nuclear cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 90 kDa

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Supplier Data

Western blot - Anti-ATR antibody (AB184137)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ATR antibody (ab184137) at 1/500 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Observed band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ATR aa 1900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13535

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/5000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATR also known as Ataxia Telangiectasia and Rad3-related protein is a serine/threonine kinase with a molecular weight of approximately 301 kDa. This protein localizes mainly in the nucleus where it functions as an important component in the cellular response to DNA damage and replication stress. ATR detects DNA strand breaks and ssDNA coated with RPA and becomes activated to phosphorylate several downstream targets initiating the DNA damage response. High expression of ATR occurs in proliferative tissues emphasizing its role in cell cycle regulation.

Biological function summary

ATR plays an essential role in maintaining genomic stability. It is part of a larger protein complex that includes ATRIP (ATR-interacting protein) which helps in localizing ATR to sites of DNA damage. Once activated ATR phosphorylates various substrates including CHK1 a critical checkpoint kinase involved in cell cycle arrest during DNA repair processes. The ability of ATR to coordinate with these proteins helps cells manage DNA damage effectively and prevent genomic instability.

Pathways

ATR functions centrally in the DNA damage response and repair mechanisms particularly the ATR-Chk1 pathway. This pathway interacts closely with the ATM (Ataxia Telangiectasia Mutated) pathway which also responds to DNA damage but usually to double-strand breaks. ATR primarily acts in response to replication stress and its activation leads to the arrest of the cell cycle allowing DNA repair to occur. This cooperation between ATR and ATM highlights their complementary roles in safeguarding genomic integrity under stress.

ATR mutations and dysregulation have strong associations with cancer and Seckel syndrome. In the context of cancer ATR often works in concert with ATM to manage DNA repair and cancer cells frequently overexpress ATR to cope with high levels of replication stress. This makes ATR a potential target for cancer therapy where its inhibition could sensitize tumor cells to chemotherapy. In Seckel syndrome ATR mutations result in developmental anomalies showcasing the important role ATR plays in cellular replication and repair processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor (PubMed : 10597277, PubMed : 10608806, PubMed : 10859164, PubMed : 11721054, PubMed : 12791985, PubMed : 12814551, PubMed : 14657349, PubMed : 14729973, PubMed : 14742437, PubMed : 15210935, PubMed : 15496423, PubMed : 16260606, PubMed : 21144835, PubMed : 21777809, PubMed : 23273981, PubMed : 25083873, PubMed : 27723717, PubMed : 27723720, PubMed : 30139873, PubMed : 33848395, PubMed : 37788673, PubMed : 37832547, PubMed : 9427750, PubMed : 9636169). Recognizes the substrate consensus sequence [ST]-Q (PubMed : 10597277, PubMed : 10608806, PubMed : 10859164, PubMed : 11721054, PubMed : 12791985, PubMed : 12814551, PubMed : 14657349, PubMed : 14729973, PubMed : 14742437, PubMed : 15210935, PubMed : 15496423, PubMed : 16260606, PubMed : 21144835, PubMed : 23273981, PubMed : 27723717, PubMed : 27723720, PubMed : 33848395, PubMed : 9427750, PubMed : 9636169). Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis (PubMed : 11114888, PubMed : 11418864, PubMed : 11865061, PubMed : 21777809, PubMed : 23273981, PubMed : 25083873, PubMed : 9925639). Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism (PubMed : 11673449). Required for FANCD2 ubiquitination (PubMed : 15314022). Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication (PubMed : 12526805). Acts as a regulator of the S-G2 transition by restricting the activity of CDK1 during S-phase to prevent premature entry into G2 (PubMed : 30139873). Acts as a regulator of the nuclear envelope integrity in response to DNA damage and stress (PubMed : 25083873, PubMed : 37788673, PubMed : 37832547). Acts as a mechanical stress sensor at the nuclear envelope : relocalizes to the nuclear envelope in response to mechanical stress and mediates a checkpoint via phosphorylation of CHEK1 (PubMed : 25083873). Also promotes nuclear envelope rupture in response to DNA damage by mediating phosphorylation of LMNA at 'Ser-282', leading to lamin disassembly (PubMed : 37832547). Involved in the inflammatory response to genome instability and double-stranded DNA breaks : acts by localizing to micronuclei arising from genome instability and catalyzing phosphorylation of LMNA at 'Ser-395', priming LMNA for subsequent phosphorylation by CDK1 and micronuclei envelope rupture (PubMed : 37788673). The rupture of micronuclear envelope triggers the cGAS-STING pathway thereby activating the type I interferon response and innate immunity (PubMed : 37788673). Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex (By similarity).

See full target information ATR

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 22:628 PubMed35672728

2022

Interfering with the expression of EEF1D gene enhances the sensitivity of ovarian cancer cells to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Qia Xu,Yun Liu,Shenyi Wang,Jing Wang,Liwei Liu,Yin Xu,Yide Qin

Molecular medicine reports 23: PubMed33537816

2021

Notoginsenoside R1 induces DNA damage via PHF6 protein to inhibit cervical carcinoma cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Cai,Wenquan Wu,Longhua Guo,Yongwu Xia,Xiaoxin Jiang,Limei Zhang,Feiding Peng,Pinghong Ming

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ATR antibody

Immunocytochemistry/ Immunofluorescence - Anti-ATR antibody (AB184137)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR antibody (AB184137)

Immunoprecipitation - Anti-ATR antibody (AB184137)

Western blot - Anti-ATR antibody (AB184137)

Western blot - Anti-ATR antibody (AB184137)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise