Rabbit Polyclonal ATR antibody. Suitable for IP, ChIP, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 47 publications. Immunogen corresponding to Fusion protein corresponding to aa 400-500 of ATR, fused to ATR.
Images
Publications
Filter by
- Genes to cells : devoted to molecular & cellular mechanisms 28:663-6732023The ribonuclease domain function is dispensable for SLFN11 to mediate cell fate decision during replication stress response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Qi,Erin Alvi,Minori Ogawa,Junya Kobayashi,Anfeng Mu,Minoru TakataPubMed 37469008
- Scientific reports 13:83042023Sting and p53 DNA repair pathways are compromised in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas J Nelson,Yunhui XuPubMed 37221295
- Oncogene 42:967-9792023BMAL1 collaborates with CLOCK to directly promote DNA double-strand break repair and tumor chemoresistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCanfeng Zhang,Liping Chen,Lu Sun,Heping Jin,Kai Ren,Shiqi Liu,Yongyu Qian,Shupeng Li,Fangping Li,Chengming Zhu,Yong Zhao,Haiying Liu,Yan LiuPubMed 36725890
- NAR cancer 5:zcac0452023ATR inhibition overcomes platinum tolerance associated with ERCC1- and p53-deficiency by inducing replication catastrophe.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoshua R Heyza,Elmira Ekinci,Jacob Lindquist,Wen Lei,Christopher Yunker,Vilvanathan Vinothkumar,Rachelle Rowbotham,Lisa Polin,Natalie G Snider,Eric Van Buren,Donovan Watza,Jessica B Back,Wei Chen,Hirva Mamdani,Ann G Schwartz,John J Turchi,Gerold Bepler,Steve M PatrickPubMed 36644397
- Nucleic acids research 50:8060-80792022LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage response signalling to trigger dual DNA repair mechanisms.Applications:WBReactive species:HumanRuyuan Yu,Yameng Hu,Shuxia Zhang,Xincheng Li,Miaoling Tang,Meisongzhu Yang,Xingui Wu,Ziwen Li,Xinyi Liao,Yingru Xu,Man Li,Suwen Chen,Wanying Qian,Li-Yun Gong,Libing Song,Jun LiPubMed 35849344
- Journal of clinical medicine 11:2022Cyclin D1 Binding Protein 1 Responds to DNA Damage through the ATM-CHK2 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYusuke Niwa,Kenya Kamimura,Kohei Ogawa,Chiyumi Oda,Yuto Tanaka,Ryoko Horigome,Masato Ohtsuka,Hiromi Miura,Koichi Fujisawa,Naoki Yamamoto,Taro Takami,Shujiro Okuda,Masayoshi Ko,Takashi Owaki,Atsushi Kimura,Osamu Shibata,Shinichi Morita,Norihiro Sakai,Hiroyuki Abe,Takeshi Yokoo,Akira Sakamaki,Hiroteru Kamimura,Shuji TeraiPubMed 35160302
- Biochemical and biophysical research communications 579:141-1452021SARS-CoV-2 triggers DNA damage response in Vero E6 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoshua Victor,Jamie Deutsch,Annalis Whitaker,Erica N Lamkin,Anthony March,Pei Zhou,Jason W Botten,Nimrat ChatterjeePubMed 34600299
- Journal of cellular biochemistry 122:1517-15332021R-loops mediate transcription-associated formation of human rDNA secondary constrictions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Zhou,Yapei Wang,Qing Wang,Le Li,Yan Hu,Yequn Wu,Mayank Gautam,Lijia LiPubMed 34224593
- Molecular cancer 20:702021CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSα/ATM-p73 axis in bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Zhang,Xingyuan Xiao,Wenjie Wei,Chao Huang,Miao Wang,Liang Wang,Yuanqiao He,Jiayin Sun,Yangkai Jiang,Guosong Jiang,Xiaoping ZhangPubMed 33874956
- The American journal of pathology 191:902-9202021Up-Regulation of DNA Damage Response Signaling in Autosomal Dominant Polycystic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer Q J Zhang,Sayanthooran Saravanabavan,Ashley N Chandra,Alexandra Munt,Annette T Y Wong,Peter C Harris,David C H Harris,Paul McKenzie,Yiping Wang,Gopala K RanganPubMed 33549515
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Fusion protein corresponding to aa 400-500 of ATR, fused to ATR. The exact immunogen used to generate this antibody is proprietary information. Database link Q13535
Reactivity data
Select an application| IP | ChIP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Expected | Tested | Expected |
| Mouse | Expected | Expected | Predicted | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes (in lysate from UV irradiated K562 cells). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor (PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11721054, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:21777809, PubMed:23273981, PubMed:25083873, PubMed:27723717, PubMed:27723720, PubMed:30139873, PubMed:33848395, PubMed:37788673, PubMed:37832547, PubMed:9427750, PubMed:9636169). Recognizes the substrate consensus sequence [ST]-Q (PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11721054, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:23273981, PubMed:27723717, PubMed:27723720, PubMed:33848395, PubMed:9427750, PubMed:9636169). Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis (PubMed:11114888, PubMed:11418864, PubMed:11865061, PubMed:21777809, PubMed:23273981, PubMed:25083873, PubMed:9925639). Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism (PubMed:11673449). Required for FANCD2 ubiquitination (PubMed:15314022). Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication (PubMed:12526805). Acts as a regulator of the S-G2 transition by restricting the activity of CDK1 during S-phase to prevent premature entry into G2 (PubMed:30139873). Acts as a regulator of the nuclear envelope integrity in response to DNA damage and stress (PubMed:25083873, PubMed:37788673, PubMed:37832547). Acts as a mechanical stress sensor at the nuclear envelope: relocalizes to the nuclear envelope in response to mechanical stress and mediates a checkpoint via phosphorylation of CHEK1 (PubMed:25083873). Also promotes nuclear envelope rupture in response to DNA damage by mediating phosphorylation of LMNA at 'Ser-282', leading to lamin disassembly (PubMed:37832547). Involved in the inflammatory response to genome instability and double-stranded DNA breaks: acts by localizing to micronuclei arising from genome instability and catalyzing phosphorylation of LMNA at 'Ser-395', priming LMNA for subsequent phosphorylation by CDK1 and micronuclei envelope rupture (PubMed:37788673). The rupture of micronuclear envelope triggers the cGAS-STING pathway thereby activating the type I interferon response and innate immunity (PubMed:37788673). Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex (By similarity).
Alternative names
FRP1, ATR, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1
Recommended products
Rabbit Polyclonal ATR antibody. Suitable for IP, ChIP, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 47 publications. Immunogen corresponding to Fusion protein corresponding to aa 400-500 of ATR, fused to ATR.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Fusion protein corresponding to aa 400-500 of ATR, fused to ATR. The exact immunogen used to generate this antibody is proprietary information. Database link Q13535
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATR also known as Ataxia Telangiectasia and Rad3-related protein is a serine/threonine kinase with a molecular weight of approximately 301 kDa. This protein localizes mainly in the nucleus where it functions as an important component in the cellular response to DNA damage and replication stress. ATR detects DNA strand breaks and ssDNA coated with RPA and becomes activated to phosphorylate several downstream targets initiating the DNA damage response. High expression of ATR occurs in proliferative tissues emphasizing its role in cell cycle regulation.
- Biological function summary
ATR plays an essential role in maintaining genomic stability. It is part of a larger protein complex that includes ATRIP (ATR-interacting protein) which helps in localizing ATR to sites of DNA damage. Once activated ATR phosphorylates various substrates including CHK1 a critical checkpoint kinase involved in cell cycle arrest during DNA repair processes. The ability of ATR to coordinate with these proteins helps cells manage DNA damage effectively and prevent genomic instability.
- Pathways
ATR functions centrally in the DNA damage response and repair mechanisms particularly the ATR-Chk1 pathway. This pathway interacts closely with the ATM (Ataxia Telangiectasia Mutated) pathway which also responds to DNA damage but usually to double-strand breaks. ATR primarily acts in response to replication stress and its activation leads to the arrest of the cell cycle allowing DNA repair to occur. This cooperation between ATR and ATM highlights their complementary roles in safeguarding genomic integrity under stress.
- Associated diseases and disorders
ATR mutations and dysregulation have strong associations with cancer and Seckel syndrome. In the context of cancer ATR often works in concert with ATM to manage DNA repair and cancer cells frequently overexpress ATR to cope with high levels of replication stress. This makes ATR a potential target for cancer therapy where its inhibition could sensitize tumor cells to chemotherapy. In Seckel syndrome ATR mutations result in developmental anomalies showcasing the important role ATR plays in cellular replication and repair processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-ATR antibody (ab2905)
Western blot on ATR in K562 whole cell lysate using ab2905.
All lanes: Western blot - Anti-ATR antibody (ab2905)
Predicted band size: 301 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com