Rabbit Recombinant Monoclonal ATR phospho T1989 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.
Images
![Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (AB237563), expandable thumbnail](https://content.abcam.com/products/images/atr-phospho-t1989-antibody-epr21991-bsa-and-azide-free-ab237563--dot-blot-img16903.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (AB237563)")
![Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (AB237563), expandable thumbnail](https://content.abcam.com/products/images/atr-phospho-t1989-antibody-epr21991-bsa-and-azide-free-ab237563--western-blot-img400038.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (AB237563)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | |
|---|---|---|
| Human | Expected | Tested |
| Synthetic peptide | Tested | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor (PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11721054, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:21777809, PubMed:23273981, PubMed:25083873, PubMed:27723717, PubMed:27723720, PubMed:30139873, PubMed:33848395, PubMed:37788673, PubMed:37832547, PubMed:9427750, PubMed:9636169). Recognizes the substrate consensus sequence [ST]-Q (PubMed:10597277, PubMed:10608806, PubMed:10859164, PubMed:11721054, PubMed:12791985, PubMed:12814551, PubMed:14657349, PubMed:14729973, PubMed:14742437, PubMed:15210935, PubMed:15496423, PubMed:16260606, PubMed:21144835, PubMed:23273981, PubMed:27723717, PubMed:27723720, PubMed:33848395, PubMed:9427750, PubMed:9636169). Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RBBP8, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis (PubMed:11114888, PubMed:11418864, PubMed:11865061, PubMed:21777809, PubMed:23273981, PubMed:25083873, PubMed:9925639). Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism (PubMed:11673449). Required for FANCD2 ubiquitination (PubMed:15314022). Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication (PubMed:12526805). Acts as a regulator of the S-G2 transition by restricting the activity of CDK1 during S-phase to prevent premature entry into G2 (PubMed:30139873). Acts as a regulator of the nuclear envelope integrity in response to DNA damage and stress (PubMed:25083873, PubMed:37788673, PubMed:37832547). Acts as a mechanical stress sensor at the nuclear envelope: relocalizes to the nuclear envelope in response to mechanical stress and mediates a checkpoint via phosphorylation of CHEK1 (PubMed:25083873). Also promotes nuclear envelope rupture in response to DNA damage by mediating phosphorylation of LMNA at 'Ser-282', leading to lamin disassembly (PubMed:37832547). Involved in the inflammatory response to genome instability and double-stranded DNA breaks: acts by localizing to micronuclei arising from genome instability and catalyzing phosphorylation of LMNA at 'Ser-395', priming LMNA for subsequent phosphorylation by CDK1 and micronuclei envelope rupture (PubMed:37788673). The rupture of micronuclear envelope triggers the cGAS-STING pathway thereby activating the type I interferon response and innate immunity (PubMed:37788673). Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex (By similarity).
Alternative names
FRP1, ATR, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1
Recommended products
Rabbit Recombinant Monoclonal ATR phospho T1989 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21991
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab237563 is the carrier-free version of Anti-ATR (phospho T1989) antibody [EPR21991] ab223258.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATR also known as Ataxia Telangiectasia and Rad3-related protein is a serine/threonine kinase with a molecular weight of approximately 301 kDa. This protein localizes mainly in the nucleus where it functions as an important component in the cellular response to DNA damage and replication stress. ATR detects DNA strand breaks and ssDNA coated with RPA and becomes activated to phosphorylate several downstream targets initiating the DNA damage response. High expression of ATR occurs in proliferative tissues emphasizing its role in cell cycle regulation.
- Biological function summary
ATR plays an essential role in maintaining genomic stability. It is part of a larger protein complex that includes ATRIP (ATR-interacting protein) which helps in localizing ATR to sites of DNA damage. Once activated ATR phosphorylates various substrates including CHK1 a critical checkpoint kinase involved in cell cycle arrest during DNA repair processes. The ability of ATR to coordinate with these proteins helps cells manage DNA damage effectively and prevent genomic instability.
- Pathways
ATR functions centrally in the DNA damage response and repair mechanisms particularly the ATR-Chk1 pathway. This pathway interacts closely with the ATM (Ataxia Telangiectasia Mutated) pathway which also responds to DNA damage but usually to double-strand breaks. ATR primarily acts in response to replication stress and its activation leads to the arrest of the cell cycle allowing DNA repair to occur. This cooperation between ATR and ATM highlights their complementary roles in safeguarding genomic integrity under stress.
- Associated diseases and disorders
ATR mutations and dysregulation have strong associations with cancer and Seckel syndrome. In the context of cancer ATR often works in concert with ATM to manage DNA repair and cancer cells frequently overexpress ATR to cope with high levels of replication stress. This makes ATR a potential target for cancer therapy where its inhibition could sensitize tumor cells to chemotherapy. In Seckel syndrome ATR mutations result in developmental anomalies showcasing the important role ATR plays in cellular replication and repair processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563), expandable thumbnail](https://content.abcam.com/products/images/atr-phospho-t1989-antibody-epr21991-bsa-and-azide-free-ab237563--dot-blot-img16903.jpg "Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)")
Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)
Dot blot analysis of ATR (phospho T1989) labeled with Anti-ATR (phospho T1989) antibody [EPR21991] ab223258 at 1/1000 dilution.
Lane 1: ATR (phospho T1989) peptide (aa1983-1992).
Lane 2: ATR (phospho T1989) peptide (aa1986-1995).
Lane 3: ATR non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATR (phospho T1989) antibody [EPR21991] ab223258).
![Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563), expandable thumbnail](https://content.abcam.com/products/images/atr-phospho-t1989-antibody-epr21991-bsa-and-azide-free-ab237563--western-blot-img400038.jpg "Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)")
Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)
Blocking and dilution buffer: 5% NFDM/TBST.
We are unsure of the nature of the 110kDa band.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATR (phospho T1989) antibody [EPR21991] ab223258).Lanes 1 - 4: Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] (Anti-ATR (phospho T1989) antibody [EPR21991] ab223258) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563) at 1/1000 dilution
Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa treated with 4mM hydroxyurea for 20 hours whole cell lysate at 15 µg
Lane 3: HeLa treated with 4mM hydroxyurea for 20 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
Lane 4: HeLa treated with 4mM hydroxyurea for 20 hours whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 270 kDa
Exposure time: 100s
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