Rabbit Polyclonal ATRAP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human AGTRAP aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AGTRAP aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6RW13
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation. Induces also a decrease in cell proliferation and angiotensin II-stimulated transcriptional activity.
Alternative names
ATRAP, AGTRAP, Type-1 angiotensin II receptor-associated protein, AT1 receptor-associated protein
Recommended products
Rabbit Polyclonal ATRAP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human AGTRAP aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AGTRAP aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6RW13
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATRAP also known as Angiotensin II Type 1 Receptor-Associated Protein functions mainly by interacting directly with the Angiotensin II Type 1 Receptor (AT1R) to modulate its signaling. It weighs approximately 17 kDa and is expressed widely in tissues particularly within the kidney cardiovascular system and adipose tissues. ATRAP influences membrane localization and activity of the AT1R impacting various cellular responses.
- Biological function summary
ATRAP limits excessive angiotensin II signaling by promoting internalization and degradation of the AT1R. Through this process it helps reduce cellular proliferation and inflammation often mediated by the receptor. ATRAP operates as a negative regulator maintaining balance in the renin-angiotensin system. It can function as part of multiprotein complexes involving AT1R and other signaling intermediates.
- Pathways
ATRAP plays a role within the renin-angiotensin-aldosterone system (RAAS) which is critical for blood pressure regulation and fluid balance. It interacts with proteins like AT1R to modulate effects of angiotensin II. The action of ATRAP affects pathways connected to oxidative stress and inflammation indicating its broader influence in cardiovascular modulation.
- Associated diseases and disorders
ATRAP has involvement in hypertension and cardiovascular disease. Its ability to modulate AT1R signaling links it to conditions characterized by heightened angiotensin II activity. Research connects ATRAP with cardiac remodeling highlighting it alongside proteins like angiotensin-converting enzyme (ACE) in disease progression. Increasing ATRAP levels may provide therapeutic benefits in managing these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-ATRAP antibody (ab251901)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for ATRAP (green) using ab251901 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRAP antibody (ab251901)
Paraffin-embedded human testis tissue stained for ATRAP using ab251901 at 1/500 dilution in immunohistochemical analysis.
Moderate cytoplasmic positivity in Leydig cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRAP antibody (ab251901)
Paraffin-embedded human liver tissue stained for ATRAP using ab251901 at 1/500 dilution in immunohistochemical analysis.
No positivity in myocytes as expected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRAP antibody (ab251901)
Paraffin-embedded human pancreas tissue stained for ATRAP using ab251901 at 1/500 dilution in immunohistochemical analysis.
Moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRAP antibody (ab251901)
Paraffin-embedded human kidney tissue stained for ATRAP using ab251901 at 1/500 dilution in immunohistochemical analysis.
Strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRAP antibody (ab251901)
Paraffin-embedded human epididymis tissue stained for ATRAP using ab251901 at 1/500 dilution in immunohistochemical analysis.
Strong cytoplasmic positivity in glandular cells.
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