Rabbit Polyclonal ATRAP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AGTRAP.
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- International journal of biological sciences 18:2452-24712022USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDandan Wang,Xiaoying Jin,Mengxia Lei,Ying Jiang,Yali Liu,Fei Yu,Yan Guo,Bing Han,Yue Yang,Weiling Sun,Ying Liu,Guangchun Zeng,Feng Liu,Jing Hu,Xuesong ChenPubMed 35414770
- Scientific reports 9:165502019Angiotensin II type 1 receptor-associated protein deficiency attenuates sirtuin1 expression in an immortalised human renal proximal tubule cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakahiro Yamaji et. al.PubMed 31719572
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human AGTRAP. The exact immunogen used to generate this antibody is proprietary information. Database link Q6RW13
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Target data
Function
Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation. Induces also a decrease in cell proliferation and angiotensin II-stimulated transcriptional activity.
Alternative names
ATRAP, AGTRAP, Type-1 angiotensin II receptor-associated protein, AT1 receptor-associated protein
Recommended products
Rabbit Polyclonal ATRAP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AGTRAP.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human AGTRAP. The exact immunogen used to generate this antibody is proprietary information. Database link Q6RW13
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- Reconstitute in 200ul sterile H2O.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATRAP also known as Angiotensin II Type 1 Receptor-Associated Protein functions mainly by interacting directly with the Angiotensin II Type 1 Receptor (AT1R) to modulate its signaling. It weighs approximately 17 kDa and is expressed widely in tissues particularly within the kidney cardiovascular system and adipose tissues. ATRAP influences membrane localization and activity of the AT1R impacting various cellular responses.
- Biological function summary
ATRAP limits excessive angiotensin II signaling by promoting internalization and degradation of the AT1R. Through this process it helps reduce cellular proliferation and inflammation often mediated by the receptor. ATRAP operates as a negative regulator maintaining balance in the renin-angiotensin system. It can function as part of multiprotein complexes involving AT1R and other signaling intermediates.
- Pathways
ATRAP plays a role within the renin-angiotensin-aldosterone system (RAAS) which is critical for blood pressure regulation and fluid balance. It interacts with proteins like AT1R to modulate effects of angiotensin II. The action of ATRAP affects pathways connected to oxidative stress and inflammation indicating its broader influence in cardiovascular modulation.
- Associated diseases and disorders
ATRAP has involvement in hypertension and cardiovascular disease. Its ability to modulate AT1R signaling links it to conditions characterized by heightened angiotensin II activity. Research connects ATRAP with cardiac remodeling highlighting it alongside proteins like angiotensin-converting enzyme (ACE) in disease progression. Increasing ATRAP levels may provide therapeutic benefits in managing these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ATRAP antibody (ab85175)
All lanes: Western blot - Anti-ATRAP antibody (ab85175) at 1/500 dilution
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: SK0V3 cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: MDA-MD-231 cell lysate
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRAP antibody (ab85175)
ab85175, at 1/100 dilution, staining ATRAP in formalin-fixed, paraffin-embedded human kidney by Immunohistochemistry. Note cytoplasmic staining.
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