AB175221

Anti-ATRIP antibody [EPR8072(2)]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal ATRIP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

AGS1, ATRIP, ATR-interacting protein, ATM and Rad3-related-interacting protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATRIP antibody [EPR8072(2)] (AB175221)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATRIP antibody [EPR8072(2)] (AB175221)

Immunofluorescent staining of MCF-7 cells labeling ATRIP with ab175221 at 1/50 dilution.

Supplier Data

Western blot - Anti-ATRIP antibody [EPR8072(2)] (AB175221)

All lanes:

Western blot - Anti-ATRIP antibody [EPR8072(2)] (ab175221) at 1/1000 dilution

Lane 1:

PC-3 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Predicted band size: 86 kDa

false

Carrier free

Anti-ATRIP antibody [EPR8072(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8072(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATRIP also known as ATRIP protein serves as an essential component within the ATR-ATRIP complex which is vital in the DNA damage response. ATRIP is a protein that weighs approximately 86 kDa and is most commonly expressed in the nucleus of cells. It functions primarily by binding to ATR (Ataxia Telangiectasia and Rad3-related protein) which enables the ATR to localize to sites of DNA damage and replication stress. This enables the recognition and repair of damaged DNA preventing genomic instability.

Biological function summary

ATRIP plays roles in maintaining genome integrity. It forms a stable complex with ATR which is important for the activation of ATR kinase activity. ATRIP directly interacts with single-stranded DNA coated by replication protein A (RPA) to perform its functions. By facilitating ATR recruitment to DNA damage sites ATRIP aids in the initiation of DNA repair mechanisms which are critical for normal cellular function and survival.

Pathways

ATRIP's activities are an integral part of the DNA damage signaling and repair pathways. ATR-ATRIP complex is central to the DNA replication checkpoint pathway where it maintains replication fork stability and controls cell cycle progression. Additionally ATRIP is involved in pathways alongside proteins like CHK1 which it helps to activate in response to DNA damage ensuring proper cell cycle arrest and repair processes.

ATRIP is implicated in certain cancers and genomic instability syndromes. Deficient ATRIP function can correlate with a higher predisposition to cancers due to defective DNA damage response and repair. Further the ATR-ATRIP interaction can influence the onset and progression of Seckel syndrome where defects in ATRIP or its partner ATR might lead to developmental abnormalities. The role of ATRIP in these diseases highlights the importance of maintaining genomic stability through effective DNA repair mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

See full target information ATRIP

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 47: PubMed33693953

2021

gene expression signature analysis revealed bosutinib as a radiosensitizer of breast cancer cells by targeting eIF4G1.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Hu,Dafei Xie,Pingkun Zhou,Xiaodan Liu,Xiaoyao Yin,Bo Huang,Hua Guan

Nature communications 11:786 PubMed32034146

2020

Acetylation of XPF by TIP60 facilitates XPF-ERCC1 complex assembly and activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Wang,Hanqing He,Binbin Chen,Guixing Jiang,Liping Cao,Haiping Jiang,Guofei Zhang,Jianxiang Chen,Jun Huang,Bing Yang,Chun Zhou,Ting Liu

Cell reports 27:1794-1808.e5 PubMed31067464

2019

Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Huang,Jing Zhang,Marina A Bellani,Durga Pokharel,Julia Gichimu,Ryan C James,Himabindu Gali,Chen Ling,Zhijiang Yan,Dongyi Xu,Junjie Chen,Amom Ruhikanta Meetei,Lei Li,Weidong Wang,Michael M Seidman

PLoS genetics 10:e1004413 PubMed24992337

2014

Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes.

Applications

ICC/IF

Species

Mouse

Jessica Hopkins,Grace Hwang,Justin Jacob,Nicklas Sapp,Rick Bedigian,Kazuhiro Oka,Paul Overbeek,Steve Murray,Philip W Jordan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATRIP antibody [EPR8072(2)]

Immunocytochemistry/ Immunofluorescence - Anti-ATRIP antibody [EPR8072(2)] (AB175221)

Western blot - Anti-ATRIP antibody [EPR8072(2)] (AB175221)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise