Rabbit Polyclonal ATRX antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 33 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATRX aa 2200-2450.
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- Aging 15:14996-150242023ATRX is a predictive marker for endocrinotherapy and chemotherapy resistance in HER2-/HR+ breast cancer through the regulation of the AR, GLI3 and GATA2 transcriptional network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Qian,Rui Ji,Cheng Shen,Yinze Wei,Chenyi Sheng,Qichao Ni,Jing Pan,Yifan Chi,Huan You,Ying Miao,Minxin Shi,Xianghua Huang,Aiguo ShenPubMed 38126976
- Nature communications 14:70902023Systemic and intrinsic functions of ATRX in glial cell fate and CNS myelination in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegan E Rowland,Yan Jiang,Sarfraz Shafiq,Alireza Ghahramani,Miguel A Pena-Ortiz,Vanessa Dumeaux,Nathalie G BérubéPubMed 37925436
- American journal of cancer research 13:3547-35582023ATRX loss suppresses the type I interferon response in sarcoma cells through chromatin remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinrui Wang,Zige Jin,Shanshan Tang,Xiyu Huang,Shanshan Wang,Xiaohe Ren,Mafei XuPubMed 37693131
- Nature communications 14:21442023Homology directed telomere clustering, ultrabright telomere formation and nuclear envelope rupture in cells lacking TRF2 and RAP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRekha Rai,Kevin Biju,Wenqi Sun,Tori Sodeinde,Amer Al-Hiyasat,Jaida Morgan,Xianwen Ye,Xueqing Li,Yong Chen,Sandy ChangPubMed 37059728
- International journal of molecular sciences 24:2023ONC201 Suppresses Neuroblastoma Growth by Interrupting Mitochondrial Function and Reactivating Nuclear ATRX Expression While Decreasing MYCN.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian-Ching Wu,Chao-Cheng Huang,Pei-Wen Wang,Ting-Ya Chen,Wen-Ming Hsu,Jiin-Haur Chuang,Hui-Ching ChuangPubMed 36675163
- Cancer research communications 2:1162-11732022Under-Representation of Racial Groups in Genomics Studies of Gastroenteropancreatic Neuroendocrine Neoplasms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrendon R Herring,Andrew Bonner,Rachael E Guenter,Selwyn Vickers,Clayton Yates,Goo Lee,Deepti Dhall,Herbert Chen,J Bart RosePubMed 36969744
- Human genome variation 9:332022The ATRX splicing variant c.21-1G>A is asymptomatic.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarin Kojima,Takahito Wada,Hiroko Shimbo,Takahiro Ikeda,Eriko F Jimbo,Hirotomo Saitsu,Naomichi Matsumoto,Takanori YamagataPubMed 36104326
- Cancers 14:2022Integrated MRI-Immune-Genomic Features Enclose a Risk Stratification Model in Patients Affected by Glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Mazzaschi,Alessandro Olivari,Antonio Pavarani,Costanza Anna Maria Lagrasta,Caterina Frati,Denise Madeddu,Bruno Lorusso,Silvia Dallasta,Chiara Tommasi,Antonino Musolino,Marcello Tiseo,Maria Michiara,Federico Quaini,Pellegrino CrafaPubMed 35805021
- Nature communications 13:34852022The chromatin remodeller ATRX facilitates diverse nuclear processes, in a stochastic manner, in both heterochromatin and euchromatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Truch,Damien J Downes,Caroline Scott,E Ravza Gür,Jelena M Telenius,Emmanouela Repapi,Ron Schwessinger,Matthew Gosden,Jill M Brown,Stephen Taylor,Pak Leng Cheong,Jim R Hughes,Douglas R Higgs,Richard J GibbonsPubMed 35710802
- Genome biology 22:3112021ATRX regulates glial identity and the tumor microenvironment in IDH-mutant glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHusam Babikir,Lin Wang,Karin Shamardani,Francisca Catalan,Sweta Sudhir,Manish K Aghi,David R Raleigh,Joanna J Phillips,Aaron A DiazPubMed 34763709
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ATRX aa 2200-2450. The exact immunogen used to generate this antibody is proprietary information. Database link P46100
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Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomeres. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according to a report, it is not sufficient to decrease chromatin condensation at telomeres nor to increase expression of telomeric RNA in fibroblasts (PubMed:24500201). May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as a negative regulator of chromatin incorporation of transcriptionally repressive histone MACROH2A1, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (PubMed:27029610).
Alternative names
RAD54L, XH2, ATRX, Transcriptional regulator ATRX, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein, Znf-HX, XNP
Recommended products
Rabbit Polyclonal ATRX antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 33 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATRX aa 2200-2450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ATRX aa 2200-2450. The exact immunogen used to generate this antibody is proprietary information. Database link P46100
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ATRX protein also recognized as alpha thalassemia/mental retardation syndrome X-linked (ATR-X) is a chromatin remodeler with a molecular weight of approximately 280 kDa. Expressed predominantly in the brain and thymus ATRX plays an important role in regulating gene expression by modulating chromatin structure. Its expression in neural tissues suggests its importance in brain development where it aids in transcriptional regulation and maintenance of genomic stability.
- Biological function summary
ATRX functions by forming part of the ATRX-DAXX complex. This complex plays a vital role in the deposition of the histone variant H3.3 into heterochromatin regions affecting the structure and function of these genomic segments. ATRX through the ATRX-DAXX complex also maintains telomere integrity by preventing uncontrolled telomere elongation. As such ATRX is important in cellular processes like DNA replication repair and mitotic progression emphasizing its role in maintaining genetic fidelity across cell divisions.
- Pathways
ATRX is an important component in chromatin remodeling and telomere maintenance pathways. It interacts closely with the DAXX protein within these pathways affecting the histone exchange mechanisms. ATRX also engages with other proteins such as p53 integrating its function into the DNA damage repair pathway. Through these pathways ATRX ensures proper chromatin dynamics and participates in mechanisms guarding genomic stability.
- Associated diseases and disorders
ATRX mutations have significant implications for conditions such as alpha thalassemia/mental retardation syndrome and certain cancers. The protein's altered functions can lead to alpha thalassemia due to improper blood cell gene expression and mental retardation from impaired brain development. In cancers mutations in ATRX often disrupt telomere maintenance contributing to oncogenesis. The relationship between ATRX and telomerase highlights the complex network through which ATRX mutations can influence telomere dynamics in cancer progression.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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10 product images
Image from Heckman, L. D., et al. eLife 3 (2014): e02676. doi: 10.7554/eLife.02676. Fig 4D. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence - Anti-ATRX antibody (ab97508)
Immunocytochemistry/ Immunofluorescence analysis of mouse brain labeling ATRX with ab97508 at 1/100 dilution. Left- DAPI (blue), middle - ATRX protein staining (red), right - merge.
Western blot - Anti-ATRX antibody (ab97508)
Western blot of (lane1) PC12 (brain)whole cell extracts, (lane 2) Rat 2 (embryo) whole cell extracts, at 30μg per lane, labeling ATRX with ab97508 at 1:1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-ATRX antibody (ab97508)
Predicted band size: 282 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (ab97508)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labeling ATRX with ab97508 at 1/500 dilution. Anti-ATRX antibody (ab97508) detects ATRX protein at nucleus on human breast carcinoma tissue sections.
Western blot - Anti-ATRX antibody (ab97508)
5% SDS-PAGE
All lanes: Western blot - Anti-ATRX antibody (ab97508) at 1/500 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: HepG2 whole cell lysate at 30 µg
Lane 3: Raji whole cell lysate at 30 µg
Predicted band size: 282 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (ab97508)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrial carcinoma labeling ATRX with ab97508 at 1/500 dilution. Anti-ATRX antibody (ab97508) detects ATRX protein at nucleus on human endometrial carcinoma tissue sections.
Immunoprecipitation - Anti-ATRX antibody (ab97508)
ab97508 at 5μg immunoprecipitating ATRX in 293T whole cell extracts.
Lane 1 (input): 293T whole cell extracts.
Lane 2 (-): Rabbit IgG instead of ab97508 in 293T whole cell extracts.
Lane 3 (+): Anti-ATRX antibody (ab97508) + 293T whole cell extracts.All lanes: Immunoprecipitation - Anti-ATRX antibody (ab97508)
Predicted band size: 282 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody (ab97508)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma,using ab97508 at 1/500 dilution.
Western blot - Anti-ATRX antibody (ab97508)
All lanes: Western blot - Anti-ATRX antibody (ab97508) at 1/1000 dilution
All lanes: NIH3T3 whole cell extracts at 30 µg
Predicted band size: 282 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ATRX antibody (ab97508)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97508 at 1/200 dilution. Lower image is merged with DNA probe.
Western blot - Anti-ATRX antibody (ab97508)
5% SDS-PAGE
All lanes: Western blot - Anti-ATRX antibody (ab97508) at 1/1000 dilution
All lanes: NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 282 kDa
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