AB188027

Anti-ATRX antibody [CL0537]

KO Validated
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(6 Publications)

Anti-ATRX antibody [CL0537] (ab188027) is a mouse monoclonal antibody detecting ATRX in Western Blot, IHC-P. Suitable for Human.

- KO validated for confirmed specificity

View Alternative Names

RAD54L, XH2, ATRX, Transcriptional regulator ATRX, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein, Znf-HX, XNP

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-ATRX antibody [CL0537] (AB188027)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ATRX with ab188027 showing clear nuclear (without nucleoli) staining in green. Microtubule are visualized in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-ATRX antibody [CL0537] (AB188027)

Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling ATRX with ab188027 showing clear nuclear (without nucleoli) staining in green. Microtubule are visualized in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-ATRX antibody [CL0537] (AB188027)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : ATRX knockout HAP1 whole cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab188027 observed at 300 kDa. Red - loading control, ab176560, observed at 50 kDa.

ab188027 was shown to recognize ATRX in wild type cells as signal was lost at the expected MW in ATRX knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ATRX knockout samples were subjected to SDS-PAGE. ab188027 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATRX antibody [CL0537] (ab188027)

Predicted band size: 282 kDa

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Supplier Data

Western blot - Anti-ATRX antibody [CL0537] (AB188027)

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

All lanes:

Western blot - Anti-ATRX antibody [CL0537] (ab188027)

Lane 1:

A-549 cells transfected with control siRNA

Lane 2:

A-549 cells transfected with target specific siRNA probe #1

Lane 3:

A-549 cells transfected with target specific siRNA probe #2

Predicted band size: 282 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0537

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human ATRX aa 2250-2450. The exact immunogen used to generate this antibody is proprietary information.

P46100

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2-10 µg/mL", "ICC-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-ATRX antibody [CL0537] (ab188027) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of ATRX?
Anti-ATRX [CL0537] (ab188027) specifically detects a band for ATRX (UniProt: P46100) at a molecular weight of 282kDa.

Specificity confirmed
The specificity of Anti-ATRX antibody [CL0537] (ab188027) has been confirmed by Western blot testing in ATRX Knockout HAP1 cells.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ATRX protein also recognized as alpha thalassemia/mental retardation syndrome X-linked (ATR-X) is a chromatin remodeler with a molecular weight of approximately 280 kDa. Expressed predominantly in the brain and thymus ATRX plays an important role in regulating gene expression by modulating chromatin structure. Its expression in neural tissues suggests its importance in brain development where it aids in transcriptional regulation and maintenance of genomic stability.

Biological function summary

ATRX functions by forming part of the ATRX-DAXX complex. This complex plays a vital role in the deposition of the histone variant H3.3 into heterochromatin regions affecting the structure and function of these genomic segments. ATRX through the ATRX-DAXX complex also maintains telomere integrity by preventing uncontrolled telomere elongation. As such ATRX is important in cellular processes like DNA replication repair and mitotic progression emphasizing its role in maintaining genetic fidelity across cell divisions.

Pathways

ATRX is an important component in chromatin remodeling and telomere maintenance pathways. It interacts closely with the DAXX protein within these pathways affecting the histone exchange mechanisms. ATRX also engages with other proteins such as p53 integrating its function into the DNA damage repair pathway. Through these pathways ATRX ensures proper chromatin dynamics and participates in mechanisms guarding genomic stability.

ATRX mutations have significant implications for conditions such as alpha thalassemia/mental retardation syndrome and certain cancers. The protein's altered functions can lead to alpha thalassemia due to improper blood cell gene expression and mental retardation from impaired brain development. In cancers mutations in ATRX often disrupt telomere maintenance contributing to oncogenesis. The relationship between ATRX and telomerase highlights the complex network through which ATRX mutations can influence telomere dynamics in cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX : DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomeres. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according to a report, it is not sufficient to decrease chromatin condensation at telomeres nor to increase expression of telomeric RNA in fibroblasts (PubMed : 24500201). May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as a negative regulator of chromatin incorporation of transcriptionally repressive histone MACROH2A1, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (PubMed : 27029610).

See full target information ATRX

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 23:104 PubMed36717819

2023

Clinical and genomic features in patients with second primary glioblastoma following first primary renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guang-Tao Zhang,Qi Liu,Fu-Xing Zuo,Hou-Jie Liu,Song-Quan Wang,Qing Yuan,Ang-Si Liu,Ke Hu,Xiao-Li Meng,Wei-Jia Wang,Hai-Peng Qian,Jing-Hai Wan,Hong-Qing Cai

Neuro-oncology advances 5:vdad003 PubMed36845293

2023

Novel genetically engineered H3.3G34R model reveals cooperation with ATRX loss in upregulation of cluster genes and promotion of neuronal lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Aalaa S Abdallah,Herminio J Cardona,Samantha L Gadd,Daniel J Brat,Plamena P Powla,Waleed S Alruwalli,Chen Shen,David J Picketts,Xiao-Nan Li,Oren J Becher

Neuro-oncology advances 3:vdab038 PubMed34013205

2021

H3K27M and promoter mutations are poor prognostic factors in surgical cases of adult thalamic high-grade glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinari Osada,Ryuta Saito,Ichiyo Shibahara,Keisuke Sasaki,Takuhiro Shoji,Masayuki Kanamori,Yukihiko Sonoda,Toshihiro Kumabe,Mika Watanabe,Teiji Tominaga

Scientific reports 11:6275 PubMed33737593

2021

Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Aiste Vitkunaite,Aida Laurinaviciene,Benoit Plancoulaine,Allan Rasmusson,Richard Levenson,Michael Shribak,Arvydas Laurinavicius

Nature communications 12:92 PubMed33397920

2021

Non-invasive assessment of telomere maintenance mechanisms in brain tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Pavithra Viswanath,Georgios Batsios,Joydeep Mukherjee,Anne Marie Gillespie,Peder E Z Larson,H Artee Luchman,Joanna J Phillips,Joseph F Costello,Russell O Pieper,Sabrina M Ronen

Journal of clinical pathology 71:702-707 PubMed29550762

2018

Phosphorylated Hsp27 is mutually exclusive with ATRX loss and the IDH1 mutation and may predict better prognosis among glioblastomas without the IDH1 mutation and ATRX loss.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Qing Cai,Peng-Fei Wang,Hai-Peng Zhang,Zhi-Jian Cheng,Shou-Wei Li,Jie He,Yu Zhang,Jia-Jie Hao,Ming-Rong Wang,Chang-Xiang Yan,Jing-Hai Wan

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ATRX antibody [CL0537]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027)

Immunocytochemistry - Anti-ATRX antibody [CL0537] (AB188027)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027)

Immunocytochemistry - Anti-ATRX antibody [CL0537] (AB188027)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027)

Western blot - Anti-ATRX antibody [CL0537] (AB188027)

Western blot - Anti-ATRX antibody [CL0537] (AB188027)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise