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Anti-ATRX antibody [CL0537] (ab188027) is a mouse monoclonal antibody that is used to detect ATRX in Western Blot, IHC-P. Suitable for Human samples.



- Specificity confirmed with ATRX knockout cell line validation

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Images

Immunocytochemistry - Anti-ATRX antibody [CL0537] (AB188027), expandable thumbnail
  • Western blot - Anti-ATRX antibody [CL0537] (AB188027), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (AB188027), expandable thumbnail
  • Immunocytochemistry - Anti-ATRX antibody [CL0537] (AB188027), expandable thumbnail
  • Western blot - Anti-ATRX antibody [CL0537] (AB188027), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human ATRX aa 2250-2450. The exact immunogen used to generate this antibody is proprietary information. Database link P46100

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCWB
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/500.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2.00000-10.00000 µg/mL
Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomeres. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according to a report, it is not sufficient to decrease chromatin condensation at telomeres nor to increase expression of telomeric RNA in fibroblasts (PubMed:24500201). May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as a negative regulator of chromatin incorporation of transcriptionally repressive histone MACROH2A1, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (PubMed:27029610).

Alternative names

RAD54L, XH2, ATRX, Transcriptional regulator ATRX, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein, Znf-HX, XNP

Recommended products

Anti-ATRX antibody [CL0537] (ab188027) is a mouse monoclonal antibody that is used to detect ATRX in Western Blot, IHC-P. Suitable for Human samples.



- Specificity confirmed with ATRX knockout cell line validation

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human ATRX aa 2250-2450. The exact immunogen used to generate this antibody is proprietary information. Database link P46100
Clone number
CL0537
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-ATRX antibody [CL0537] (ab188027) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), in Human samples.

What is the molecular weight of ATRX?


Anti-ATRX [CL0537] (ab188027) specifically detects a band for ATRX (UniProt: P46100) at a molecular weight of 282kDa.

Recommended positive controls


IHC-P: Human brain glioma, fallopian tube, and testis tissues; WB: A-549 cell lysate.ICC: A431 and HeLa cells.

Specificity confirmed


The specificity of Anti-ATRX antibody [CL0537] (ab188027) has been confirmed by Western blot testing in ATRX Knockout HAP1 cells.



Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ATRX protein also recognized as alpha thalassemia/mental retardation syndrome X-linked (ATR-X) is a chromatin remodeler with a molecular weight of approximately 280 kDa. Expressed predominantly in the brain and thymus ATRX plays an important role in regulating gene expression by modulating chromatin structure. Its expression in neural tissues suggests its importance in brain development where it aids in transcriptional regulation and maintenance of genomic stability.

Biological function summary

ATRX functions by forming part of the ATRX-DAXX complex. This complex plays a vital role in the deposition of the histone variant H3.3 into heterochromatin regions affecting the structure and function of these genomic segments. ATRX through the ATRX-DAXX complex also maintains telomere integrity by preventing uncontrolled telomere elongation. As such ATRX is important in cellular processes like DNA replication repair and mitotic progression emphasizing its role in maintaining genetic fidelity across cell divisions.

Pathways

ATRX is an important component in chromatin remodeling and telomere maintenance pathways. It interacts closely with the DAXX protein within these pathways affecting the histone exchange mechanisms. ATRX also engages with other proteins such as p53 integrating its function into the DNA damage repair pathway. Through these pathways ATRX ensures proper chromatin dynamics and participates in mechanisms guarding genomic stability.

Associated diseases and disorders

ATRX mutations have significant implications for conditions such as alpha thalassemia/mental retardation syndrome and certain cancers. The protein's altered functions can lead to alpha thalassemia due to improper blood cell gene expression and mental retardation from impaired brain development. In cancers mutations in ATRX often disrupt telomere maintenance contributing to oncogenesis. The relationship between ATRX and telomerase highlights the complex network through which ATRX mutations can influence telomere dynamics in cancer progression.

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8 product images

  • Immunocytochemistry - Anti-ATRX antibody [CL0537] (ab188027), expandable thumbnail

    Immunocytochemistry - Anti-ATRX antibody [CL0537] (ab188027)

    Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling ATRX with ab188027 showing clear nuclear (without nucleoli) staining in green. Microtubule are visualized in red.

  • Western blot - Anti-ATRX antibody [CL0537] (ab188027), expandable thumbnail

    Western blot - Anti-ATRX antibody [CL0537] (ab188027)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: ATRX knockout HAP1 whole cell lysate (20 μg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab188027 observed at 300 kDa. Red - loading control, Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560, observed at 50 kDa.

    ab188027 was shown to recognize ATRX in wild type cells as signal was lost at the expected MW in ATRX knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ATRX knockout samples were subjected to SDS-PAGE. ab188027 and Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ATRX antibody [CL0537] (ab188027)

    Predicted band size: 282 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (ab188027), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (ab188027)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-ATRX antibody [CL0537] (ab188027), expandable thumbnail

    Immunocytochemistry - Anti-ATRX antibody [CL0537] (ab188027)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ATRX with ab188027 showing clear nuclear (without nucleoli) staining in green. Microtubule are visualized in red.

  • Western blot - Anti-ATRX antibody [CL0537] (ab188027), expandable thumbnail

    Western blot - Anti-ATRX antibody [CL0537] (ab188027)

    Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

    All lanes: Western blot - Anti-ATRX antibody [CL0537] (ab188027)

    Lane 1: A-549 cells transfected with control siRNA

    Lane 2: A-549 cells transfected with target specific siRNA probe #1

    Lane 3: A-549 cells transfected with target specific siRNA probe #2

    Predicted band size: 282 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (ab188027), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (ab188027)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (ab188027), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (ab188027)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (ab188027), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATRX antibody [CL0537] (ab188027)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling ATRX with ab188027 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

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