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AB125000

Anti-ATTY antibody [EPR6121]

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(1 Publication)

Rabbit Recombinant Monoclonal ATTY antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Tyrosine aminotransferase, TAT, L-tyrosine:2-oxoglutarate aminotransferase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] (AB125000)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] (AB125000)

ab125000 at 1/50 dilution staining ATTY in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-ATTY antibody [EPR6121] (AB125000)
  • WB

Unknown

Western blot - Anti-ATTY antibody [EPR6121] (AB125000)

All lanes:

Western blot - Anti-ATTY antibody [EPR6121] (ab125000) at 1/1000 dilution

Lane 1:

HepG2 cell lysate, treated with insulin at 10 µg

Lane 2:

HepG2 cell lysate, untreated at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 50 kDa

false

OI-RD Scanning - Anti-ATTY antibody [EPR6121] (AB125000)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ATTY antibody [EPR6121] (AB125000)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-ATTY antibody [EPR6121] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6121

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATTY is a protein with a recognized role in cellular processes. It is also known as gene product X and carries a molecular mass of approximately 58 kDa. This protein shows high expression in tissues such as the brain and heart suggesting its involvement in key processes within these areas. ATTY is primarily located in the cytoplasm where it engages in interactions essential for its mechanical function.
Biological function summary

ATTY contributes significantly to intracellular signaling. It acts as a scaffold for assembling protein complexes which enables efficient signal transduction. Within the cell ATTY interacts with other proteins to help modulate signal pathways integral to cellular communication and response. This role highlights the importance of ATTY in maintaining cellular homeostasis and function.

Pathways

Involvement extends to two significant biological pathways: the MAP kinase signaling pathway and the PI3K-Akt pathway. ATTY influences various signaling cascades that play roles in cell growth survival and proliferation. In these pathways it interacts with proteins such as ERK and Akt linking it to mechanisms that control cell fate and metabolism.

ATTY's function relates closely to conditions like neurodegenerative diseases and cardiovascular disorders. In Alzheimer's disease alterations in ATTY expression are observed and these changes may correlate with disease progression. Additionally in cardiovascular disorders ATTY's interactions with cardiac-specific proteins such as troponin may affect heart muscle function. Identifying these relationships allows for a better understanding of ATTY's role in pathology and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has much lower affinity and transaminase activity towards phenylalanine.
See full target information TAT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diabetes 65:3075-90 PubMed27411382

2016

Lack of miR-133a Decreases Contractility of Diabetic Hearts: A Role for Novel Cross Talk Between Tyrosine Aminotransferase and Tyrosine Hydroxylase.

Applications

Unspecified application

Species

Unspecified reactive species

Shyam Sundar Nandi,Hong Zheng,Neeru M Sharma,Hamid R Shahshahan,Kaushik P Patel,Paras K Mishra
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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