Rabbit Recombinant Monoclonal ATTY antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-ATTY antibody [EPR6121] - BSA and Azide free (AB248092), expandable thumbnail](https://content.abcam.com/products/images/atty-antibody-epr6121-bsa-and-azide-free-ab248092--western-blot-img99400.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATTY antibody [EPR6121] - BSA and Azide free (AB248092)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] - BSA and Azide free (AB248092), expandable thumbnail](https://content.abcam.com/products/images/atty-antibody-epr6121-bsa-and-azide-free-ab248092--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116566.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] - BSA and Azide free (AB248092)")
![OI-RD Scanning - Anti-ATTY antibody [EPR6121] - BSA and Azide free (AB248092), expandable thumbnail](https://content.abcam.com/products/images/atty-antibody-epr6121-bsa-and-azide-free-ab248092--oi-rd-scanning-img95853.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-ATTY antibody [EPR6121] - BSA and Azide free (AB248092)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has much lower affinity and transaminase activity towards phenylalanine.
Alternative names
Tyrosine aminotransferase, TAT, L-tyrosine:2-oxoglutarate aminotransferase
Recommended products
Rabbit Recombinant Monoclonal ATTY antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6121
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 4.19 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248092 is the carrier-free version of Anti-ATTY antibody [EPR6121] ab125000.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATTY is a protein with a recognized role in cellular processes. It is also known as gene product X and carries a molecular mass of approximately 58 kDa. This protein shows high expression in tissues such as the brain and heart suggesting its involvement in key processes within these areas. ATTY is primarily located in the cytoplasm where it engages in interactions essential for its mechanical function.
- Biological function summary
ATTY contributes significantly to intracellular signaling. It acts as a scaffold for assembling protein complexes which enables efficient signal transduction. Within the cell ATTY interacts with other proteins to help modulate signal pathways integral to cellular communication and response. This role highlights the importance of ATTY in maintaining cellular homeostasis and function.
- Pathways
Involvement extends to two significant biological pathways: the MAP kinase signaling pathway and the PI3K-Akt pathway. ATTY influences various signaling cascades that play roles in cell growth survival and proliferation. In these pathways it interacts with proteins such as ERK and Akt linking it to mechanisms that control cell fate and metabolism.
- Associated diseases and disorders
ATTY's function relates closely to conditions like neurodegenerative diseases and cardiovascular disorders. In Alzheimer's disease alterations in ATTY expression are observed and these changes may correlate with disease progression. Additionally in cardiovascular disorders ATTY's interactions with cardiac-specific proteins such as troponin may affect heart muscle function. Identifying these relationships allows for a better understanding of ATTY's role in pathology and potential therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ATTY antibody [EPR6121] - BSA and Azide free (ab248092), expandable thumbnail](https://content.abcam.com/products/images/atty-antibody-epr6121-bsa-and-azide-free-ab248092--western-blot-img99400.jpg "Western blot - Anti-ATTY antibody [EPR6121] - BSA and Azide free (ab248092)")
Western blot - Anti-ATTY antibody [EPR6121] - BSA and Azide free (ab248092)
This data was developed using Anti-ATTY antibody [EPR6121] ab125000, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ATTY antibody [EPR6121] (Anti-ATTY antibody [EPR6121] ab125000) at 1/1000 dilution
Lane 1: HepG2 cell lysate, treated with insulin at 10 µg
Lane 2: HepG2 cell lysate, untreated at 10 µg
Lane 3: A549 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] - BSA and Azide free (ab248092), expandable thumbnail](https://content.abcam.com/products/images/atty-antibody-epr6121-bsa-and-azide-free-ab248092--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116566.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] - BSA and Azide free (ab248092)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATTY antibody [EPR6121] - BSA and Azide free (ab248092)
This data was developed using Anti-ATTY antibody [EPR6121] ab125000, the same antibody clone in a different buffer formulation.Anti-ATTY antibody [EPR6121] ab125000 at 1/50 dilution staining ATTY in paraffin-embedded Human brain tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-ATTY antibody [EPR6121] - BSA and Azide free (ab248092), expandable thumbnail](https://content.abcam.com/products/images/atty-antibody-epr6121-bsa-and-azide-free-ab248092--oi-rd-scanning-img95853.jpg "OI-RD Scanning - Anti-ATTY antibody [EPR6121] - BSA and Azide free (ab248092)")
OI-RD Scanning - Anti-ATTY antibody [EPR6121] - BSA and Azide free (ab248092)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com