AB99947

Anti-ATXN7L3 antibody

Name: Anti-ATXN7L3 antibody (ab99947) | Abcam
Brand: Abcam Limited
SKU: AB99947
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal ATXN7L3 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ATXN7L3 aa 250-350.

View Alternative Names

Ataxin-7-like protein 3, SAGA-associated factor 11 homolog, ATXN7L3

2 Images

Supplier Data

Western blot - Anti-ATXN7L3 antibody (AB99947)

All lanes:

Western blot - Anti-ATXN7L3 antibody (ab99947) at 0.4 µg/mL

All lanes:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 38 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-ATXN7L3 antibody (AB99947)

All lanes:

Western blot - Anti-ATXN7L3 antibody (ab99947) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK293T whole cell lysate at 50 µg

Lane 5:

HEK293T whole cell lysate at 15 µg

Predicted band size: 38 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human ATXN7L3 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q14CW9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab99947 was affinity purified using an epitope specific to ATXN7L3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATXN7L3 is a protein that plays a significant mechanical role in the regulation of gene expression. Also known as ataxin-7-like protein 3 ATXN7L3 has a molecular mass of approximately 36 kDa. The protein is expressed in various human tissues including the brain heart and muscles. Its function includes modifying histones which affects the accessibility of transcription factors to DNA influencing the transcriptional activity of certain genes.

Biological function summary

ATXN7L3 is a component of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex which is involved in chromatin remodeling and transcriptional regulation. This protein facilitates the deubiquitination of histones a process that modifies histone proteins and influences chromatin structure impacting gene expression. The participation of ATXN7L3 in the SAGA complex highlights its role in enabling the transcription of genes by altering the chromatin landscape.

Pathways

ATXN7L3 influences transcription regulation pathways by its action within the SAGA complex. The protein engages with histone modification pathways notably involved with the activity of deubiquitination enzymes. Related proteins include USP22 which partners with ATXN7L3 in removing ubiquitin groups from histones leading to important changes in the gene expression patterns.

ATXN7L3 associates with neurodegenerative diseases such as spinocerebellar ataxia type 7. This condition results from mutations affecting the ATXN7 gene and involves abnormal expansions of CAG trinucleotide repeats. The interaction with proteins like ATXN7 within the polyglutamine tract expansion disorder category is relevant for understanding the pathological mechanisms underlying these diseases. This connection makes ATXN7L3 a potential target for therapeutic interventions in related neurodegenerative conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B (PubMed : 18206972, PubMed : 21746879). The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. Within the complex, it is required to recruit USP22 and ENY2 into the SAGA complex (PubMed : 18206972). Regulates H2B monoubiquitination (H2Bub1) levels. Affects subcellular distribution of ENY2, USP22 and ATXN7L3B (PubMed : 27601583).

See full target information ATXN7L3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9550 PubMed39500869

2024

Inhibition of GPX4 enhances CDK4/6 inhibitor and endocrine therapy activity in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

M T Herrera-Abreu,J Guan,U Khalid,J Ning,M R Costa,J Chan,Q Li,J-P Fortin,W R Wong,P Perampalam,A Biton,W Sandoval,J Vijay,M Hafner,R Cutts,G Wilson,J Frankum,T I Roumeliotis,J Alexander,O Hickman,R Brough,S Haider,J Choudhary,C J Lord,A Swain,C Metcalfe,N C Turner

Bioscience reports 40: PubMed31737900

2019

Long non-coding RNA DSCAM-AS1 contributes to the tumorigenesis of cervical cancer by targeting miR-877-5p/ATXN7L3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liang,Shujuan Zhang,Wei Wang,Yan Xu,Atikan Kawuli,Jiaqi Lu,Xuemei Xiu

Oncogene 36:5819-5828 PubMed28604753

2017

let-7b and let-7c microRNAs promote histone H2B ubiquitylation and inhibit cell migration by targeting multiple components of the H2B deubiquitylation machinery.

Applications

Species

Unspecified reactive species

A Spolverini,G Fuchs,D R Bublik,M Oren

View all publications

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Product promise

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Anti-ATXN7L3 antibody

Western blot - Anti-ATXN7L3 antibody (AB99947)

Western blot - Anti-ATXN7L3 antibody (AB99947)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise