AB155980

Anti-AUH antibody [EPR11087(B)]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal AUH antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

3-MG-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, Itaconyl-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AUH

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

ab155980 showing +ve staining in Human endometrial carcinoma.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue labeling AUH using ab155980 at a 1/50 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

ab155980 showing +ve staining in Human normal heart.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

ab155980 showing +ve staining in Human normal brain.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

ab155980 showing +ve staining in Human thyroid gland carcinoma.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] (AB155980)

ab155980 showing +ve staining in Human normal kidney.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-AUH antibody [EPR11087(B)] (AB155980)

WB

Unknown

Western blot - Anti-AUH antibody [EPR11087(B)] (AB155980)

All lanes:

Western blot - Anti-AUH antibody [EPR11087(B)] (ab155980) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH SY5Y lysate at 10 µg

Predicted band size: 36 kDa

false

Carrier free

Anti-AUH antibody [EPR11087(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11087(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AUH also known as AU-specific RNA binding protein/enoyl-CoA hydratase is a bifunctional protein with a molecular mass of approximately 39 kDa. It plays an important role in RNA binding and hydratase activities. AUH is expressed in many tissues including liver brain and heart providing its multiple functional capacities within cells. It binds to AU-rich elements present in mRNA which has significant influence on mRNA stability and turnover. The protein also acts as an enoyl-CoA hydratase involved in the leucine catabolic process.

Biological function summary

The AUH protein is involved in regulating the stability of mRNA by binding to AU-rich elements. This activity is critical for controlling genes expression as it modulates the decay rate of various mRNAs involved in cellular responses. While AUH functions largely independently it may interact with other proteins transiently to exert its RNA binding activity although it is not a part of a stable protein complex. Its hydratase function contributes to the breaking down of leucine an essential amino acid further highlighting its multifaceted biological roles.

Pathways

AUH is interwoven with essential metabolic and gene expression pathways. The protein aligns heavily within the leucine catabolism pathway contributing to the conversion of leucine to acetoacetate and acetyl-CoA which are key intermediates in energy production. AUH's involvement in mRNA decay processes connects it to pathways regulating apoptosis and proliferation where precise control of mRNA turnover by AUH and its related proteins like HuR is essential for cellular outcome.

AUH is linked to a rare genetic disorder known as 3-Methylglutaconic aciduria type 1 (MGA-1) characterized by metabolic anomalies manifesting due to defective leucine catabolism. Mutations in the AUH gene disrupt normal protein function leading to accumulation of metabolic intermediates. Furthermore AUH's role in mRNA regulation implies a connection to cancer where altered expression or mutation might contribute to disruptions in gene expression control. Associated proteins in these contexts include components of the mRNA decay pathway and enzymes involved in amino acid metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the fifth step in the leucine degradation pathway, the reversible hydration of 3-methylglutaconyl-CoA (3-MG-CoA) to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) (PubMed : 11738050, PubMed : 12434311, PubMed : 12655555, PubMed : 16640564). Can catalyze the reverse reaction but at a much lower rate in vitro (PubMed : 16640564). HMG-CoA is then quickly degraded by another enzyme (such as HMG-CoA lyase) to give acetyl-CoA and acetoacetate (PubMed : 16640564). Uses other substrates such as (2E)-glutaconyl-CoA efficiently in vitro, and to a lesser extent 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA), crotonyl-CoA ((2E)-butenoyl-CoA) and 3-hydroxybutanoyl-CoA (the missing carboxylate reduces affinity to the active site) (PubMed : 16640564). Originally it was identified as an RNA-binding protein as it binds to AU-rich elements (AREs) in vitro (PubMed : 7892223). AREs direct rapid RNA degradation and mRNA deadenylation (PubMed : 7892223). Might have itaconyl-CoA hydratase activity, converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway (PubMed : 29056341). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, an antimicrobial metabolite and immunomodulator produced by macrophages during certain infections, that can act as a vitamin B12-poisoning metabolite (PubMed : 29056341).

See full target information Methylglutaconyl-CoA hydratase, mitochondrial

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology of sex differences 10:8 PubMed30728084

2019

Sex-specific regulation of cardiac microRNAs targeting mitochondrial proteins in pressure overload.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo Sanchez-Ruderisch,Ana Maria Queirós,Daniela Fliegner,Claudia Eschen,Georgios Kararigas,Vera Regitz-Zagrosek

View all publications

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Product promise

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