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AB215823

Anti-AUH antibody [EPR11087(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal AUH antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

3-MG-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, Itaconyl-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AUH

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)

This data was developed using ab155980, the same antibody clone in a different buffer formulation.

ab155980 showing +ve staining in Human endometrial carcinoma.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)

This data was developed using ab155980, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling AUH using ab155980 at a 1/50 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)

This data was developed using ab155980, the same antibody clone in a different buffer formulation.

ab155980 showing +ve staining in Human normal heart.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)

This data was developed using ab155980, the same antibody clone in a different buffer formulation.

ab155980 showing +ve staining in Human normal brain.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)

This data was developed using ab155980, the same antibody clone in a different buffer formulation.

ab155980 showing +ve staining in Human thyroid gland carcinoma.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)

This data was developed using ab155980, the same antibody clone in a different buffer formulation.

ab155980 showing +ve staining in Human normal kidney.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)
  • WB

Unknown

Western blot - Anti-AUH antibody [EPR11087(B)] - BSA and Azide free (AB215823)

This data was developed using ab155980, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AUH antibody [EPR11087(B)] (<a href='/en-us/products/primary-antibodies/auh-antibody-epr11087b-ab155980'>ab155980</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH SY5Y lysate at 10 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11087(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab215823 is the carrier-free version of ab155980.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AUH also known as AU-specific RNA binding protein/enoyl-CoA hydratase is a bifunctional protein with a molecular mass of approximately 39 kDa. It plays an important role in RNA binding and hydratase activities. AUH is expressed in many tissues including liver brain and heart providing its multiple functional capacities within cells. It binds to AU-rich elements present in mRNA which has significant influence on mRNA stability and turnover. The protein also acts as an enoyl-CoA hydratase involved in the leucine catabolic process.
Biological function summary

The AUH protein is involved in regulating the stability of mRNA by binding to AU-rich elements. This activity is critical for controlling genes expression as it modulates the decay rate of various mRNAs involved in cellular responses. While AUH functions largely independently it may interact with other proteins transiently to exert its RNA binding activity although it is not a part of a stable protein complex. Its hydratase function contributes to the breaking down of leucine an essential amino acid further highlighting its multifaceted biological roles.

Pathways

AUH is interwoven with essential metabolic and gene expression pathways. The protein aligns heavily within the leucine catabolism pathway contributing to the conversion of leucine to acetoacetate and acetyl-CoA which are key intermediates in energy production. AUH's involvement in mRNA decay processes connects it to pathways regulating apoptosis and proliferation where precise control of mRNA turnover by AUH and its related proteins like HuR is essential for cellular outcome.

AUH is linked to a rare genetic disorder known as 3-Methylglutaconic aciduria type 1 (MGA-1) characterized by metabolic anomalies manifesting due to defective leucine catabolism. Mutations in the AUH gene disrupt normal protein function leading to accumulation of metabolic intermediates. Furthermore AUH's role in mRNA regulation implies a connection to cancer where altered expression or mutation might contribute to disruptions in gene expression control. Associated proteins in these contexts include components of the mRNA decay pathway and enzymes involved in amino acid metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the fifth step in the leucine degradation pathway, the reversible hydration of 3-methylglutaconyl-CoA (3-MG-CoA) to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) (PubMed : 11738050, PubMed : 12434311, PubMed : 12655555, PubMed : 16640564). Can catalyze the reverse reaction but at a much lower rate in vitro (PubMed : 16640564). HMG-CoA is then quickly degraded by another enzyme (such as HMG-CoA lyase) to give acetyl-CoA and acetoacetate (PubMed : 16640564). Uses other substrates such as (2E)-glutaconyl-CoA efficiently in vitro, and to a lesser extent 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA), crotonyl-CoA ((2E)-butenoyl-CoA) and 3-hydroxybutanoyl-CoA (the missing carboxylate reduces affinity to the active site) (PubMed : 16640564). Originally it was identified as an RNA-binding protein as it binds to AU-rich elements (AREs) in vitro (PubMed : 7892223). AREs direct rapid RNA degradation and mRNA deadenylation (PubMed : 7892223). Might have itaconyl-CoA hydratase activity, converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway (PubMed : 29056341). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, an antimicrobial metabolite and immunomodulator produced by macrophages during certain infections, that can act as a vitamin B12-poisoning metabolite (PubMed : 29056341).
See full target information Methylglutaconyl-CoA hydratase, mitochondrial
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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