AB61114

Anti-Aurora A antibody

Name: Anti-Aurora A antibody (ab61114) | Abcam
Brand: Abcam Limited
SKU: AB61114
Price: 565 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(5 Publications)

Rabbit Polyclonal Aurora A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human AURKA.

View Alternative Names

AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6, AURKA, Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, Breast tumor-amplified kinase, Ipl1- and aurora-related kinase 1, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase Ayk1, Serine/threonine-protein kinase aurora-A, ARK-1, Aurora-related kinase 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora A antibody (AB61114)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora A antibody (AB61114)

ab61114 staining Aurora A in human ovarian carcinoma.
Left panel : with primary antibody at 4 ug/ml. Right panel : isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

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Western blot - Anti-Aurora A antibody (AB61114)

All lanes:

Western blot - Anti-Aurora A antibody (ab61114) at 1/500 dilution

Lane 1:

Extracts from 293 cells treated with serum (20%, 15mins)

Lane 2:

Extracts from 293 cells treated with serum (20%, 15mins) with immunising Aurora A peptide

Predicted band size: 45 kDa

Observed band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human AURKA. The exact immunogen used to generate this antibody is proprietary information.

O14965

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab61114 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aurora A also known as Aurora kinase A is an important serine/threonine kinase with a protein mass of approximately 46 kDa. It is commonly found in various tissues but its expression is particularly high during mitosis in dividing cells. Aurora A operates primarily at the centrosomes where it is pivotal for processes such as centrosome maturation separation and the establishment of the mitotic spindle. The kinase activity of Aurora A is important for proper chromosome alignment and segregation. Scientists often use Aurora A as a marker in studies related to cell division and cancer.

Biological function summary

Aurora A plays key roles in the regulation of the cell cycle and mitosis. It functions as part of the Aurora mouse kinase complex associating with other proteins to ensure accurate chromosomal segregation and cytokinesis. Aurora A is involved in centrosome dynamics and interacts with proteins such as 35C1 aiding its localization to the correct cellular structures. The protein’s role in the cell cycle and mitotic checkpoints makes it a subject of interest for researchers examining cancer proliferation.

Pathways

Aurora A integrates into both the mitotic spindle assembly checkpoint and DNA damage repair pathways. Within these pathways Aurora A partners with proteins like APC (Anaphase Promoting Complex) to ensure timely progression through mitosis and safeguard genomic stability. The spindle assembly pathway ensures that spindle fibers correctly attach to chromosomes a process in which Aurora A proves instrumental. Its interaction with other kinases and regulatory molecules in these pathways highlights its regulatory importance in cell proliferation.

Research identifies Aurora A's association with oncological conditions such as breast and ovarian cancers. Overexpression or amplification of Aurora A correlates with tumor progression and poor prognosis as it can lead to chromosomal instability. In these diseases Aurora A activity is linked with dysregulated pathways involving proteins like p53 and BRCA1 which normally function in DNA damage response and repair. Targeted inhibition of Aurora A represents a promising avenue in cancer therapy as it might restore normal cell cycle control and reduce cancer cell viability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression (PubMed : 11039908, PubMed : 12390251, PubMed : 17125279, PubMed : 17360485, PubMed : 18615013, PubMed : 26246606). Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis (PubMed : 14523000, PubMed : 26246606). Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase (PubMed : 27335426). Required for initial activation of CDK1 at centrosomes (PubMed : 13678582, PubMed : 15128871). Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2 (PubMed : 11551964, PubMed : 14702041, PubMed : 15128871, PubMed : 15147269, PubMed : 15987997, PubMed : 17604723, PubMed : 18056443, PubMed : 18615013). Phosphorylates MCRS1 which is required for MCRS1-mediated kinetochore fiber assembly and mitotic progression (PubMed : 27192185). Regulates KIF2A tubulin depolymerase activity (PubMed : 19351716). Important for microtubule formation and/or stabilization (PubMed : 18056443). Required for normal axon formation (PubMed : 19812038). Plays a role in microtubule remodeling during neurite extension (PubMed : 19668197). Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53 (PubMed : 14702041). Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity (PubMed : 11551964). Inhibits cilia outgrowth (By similarity). Required for cilia disassembly via phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed : 17604723, PubMed : 20643351). Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1 (PubMed : 28218735).

See full target information AURKA

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 15:3693-3711 PubMed40948526

2025

Aurora-A inhibits hepatocellular carcinoma cell ferroptosis to mediate immune escape by disrupting phosphatidylethanolamine biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Fan,Yiqian Liu,Yucheng Cai,Xinnan Sun,Jiaxuan Li,Yiyang Xu,Changchun Sun,Shiyun Cui

International journal of molecular sciences 25: PubMed38892390

2024

Involvement of Endolysosomes and Aurora Kinase A in the Regulation of Amyloid β Protein Levels in Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Zahra Afghah,Nabab Khan,Gaurav Datta,Peter W Halcrow,Jonathan D Geiger,Xuesong Chen

Heliyon 8:e11656 PubMed36458309

2022

PEITC: A resounding molecule averts metastasis in breast cancer cells by regulating PKCδ/Aurora A interplay.

Applications

Unspecified application

Species

Unspecified reactive species

Souvick Biswas,Elizabeth Mahapatra,Salini Das,Madhumita Roy,Sutapa Mukherjee

Bioengineered 12:8296-8308 PubMed34565287

2021

Silencing Aurora-kinase-A (AURKA) reinforced the sensitivity of diffuse large B-cell lymphoma cells to cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) via suppressing β-Catenin and RAS-extracellular signal-regulated protein kinase (ERK1/2) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoxiong Wang,Li Sun

Arthritis and rheumatism 65:1725-35 PubMed23653330

2013

Differentially expressed epigenome modifiers, including aurora kinases A and B, in immune cells in rheumatoid arthritis in humans and mouse models.

Applications

Species

Mouse

Tibor T Glant,Timea Besenyei,András Kádár,Júlia Kurkó,Beata Tryniszewska,János Gál,Györgyi Soós,Zoltán Szekanecz,Gyula Hoffmann,Joel A Block,Robert S Katz,Katalin Mikecz,Tibor A Rauch

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aurora A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora A antibody (AB61114)

Western blot - Anti-Aurora A antibody (AB61114)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise