AB1287

Anti-Aurora A antibody - Centrosome Marker

Name: Anti-Aurora A antibody - Centrosome Marker (ab1287) | Abcam
Brand: Abcam Limited
SKU: AB1287
Price: 400 USD
Availability: InStock
Rating: 4 (1 reviews)

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(45 Publications)

Rabbit Polyclonal Aurora A antibody. Centrosome marker. Suitable for IP and reacts with Human samples. Cited in 45 publications. Immunogen corresponding to Synthetic Peptide within Human AURKA conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6, AURKA, Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, Breast tumor-amplified kinase, Ipl1- and aurora-related kinase 1, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase Ayk1, Serine/threonine-protein kinase aurora-A, ARK-1, Aurora-related kinase 1

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Supplier Data

Immunoprecipitation - Anti-Aurora A antibody - Centrosome Marker (AB1287)

Immunoprecipitation of Human Aurora A with ab1287 at 0.1 μg/mL.

Lane 1 : ab1287 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Lane 2 : IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Detection : Chemiluminescence

All lanes:

Immunoprecipitation - Anti-Aurora A antibody - Centrosome Marker (ab1287)

Predicted band size: 45 kDa

false

Exposure time: 75s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human AURKA conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O14965

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "0.1 µg/mL", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aurora A also known as Aurora kinase A is an important serine/threonine kinase with a protein mass of approximately 46 kDa. It is commonly found in various tissues but its expression is particularly high during mitosis in dividing cells. Aurora A operates primarily at the centrosomes where it is pivotal for processes such as centrosome maturation separation and the establishment of the mitotic spindle. The kinase activity of Aurora A is important for proper chromosome alignment and segregation. Scientists often use Aurora A as a marker in studies related to cell division and cancer.

Biological function summary

Aurora A plays key roles in the regulation of the cell cycle and mitosis. It functions as part of the Aurora mouse kinase complex associating with other proteins to ensure accurate chromosomal segregation and cytokinesis. Aurora A is involved in centrosome dynamics and interacts with proteins such as 35C1 aiding its localization to the correct cellular structures. The protein’s role in the cell cycle and mitotic checkpoints makes it a subject of interest for researchers examining cancer proliferation.

Pathways

Aurora A integrates into both the mitotic spindle assembly checkpoint and DNA damage repair pathways. Within these pathways Aurora A partners with proteins like APC (Anaphase Promoting Complex) to ensure timely progression through mitosis and safeguard genomic stability. The spindle assembly pathway ensures that spindle fibers correctly attach to chromosomes a process in which Aurora A proves instrumental. Its interaction with other kinases and regulatory molecules in these pathways highlights its regulatory importance in cell proliferation.

Research identifies Aurora A's association with oncological conditions such as breast and ovarian cancers. Overexpression or amplification of Aurora A correlates with tumor progression and poor prognosis as it can lead to chromosomal instability. In these diseases Aurora A activity is linked with dysregulated pathways involving proteins like p53 and BRCA1 which normally function in DNA damage response and repair. Targeted inhibition of Aurora A represents a promising avenue in cancer therapy as it might restore normal cell cycle control and reduce cancer cell viability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression (PubMed : 11039908, PubMed : 12390251, PubMed : 17125279, PubMed : 17360485, PubMed : 18615013, PubMed : 26246606). Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis (PubMed : 14523000, PubMed : 26246606). Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase (PubMed : 27335426). Required for initial activation of CDK1 at centrosomes (PubMed : 13678582, PubMed : 15128871). Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2 (PubMed : 11551964, PubMed : 14702041, PubMed : 15128871, PubMed : 15147269, PubMed : 15987997, PubMed : 17604723, PubMed : 18056443, PubMed : 18615013). Phosphorylates MCRS1 which is required for MCRS1-mediated kinetochore fiber assembly and mitotic progression (PubMed : 27192185). Regulates KIF2A tubulin depolymerase activity (PubMed : 19351716). Important for microtubule formation and/or stabilization (PubMed : 18056443). Required for normal axon formation (PubMed : 19812038). Plays a role in microtubule remodeling during neurite extension (PubMed : 19668197). Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53 (PubMed : 14702041). Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity (PubMed : 11551964). Inhibits cilia outgrowth (By similarity). Required for cilia disassembly via phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed : 17604723, PubMed : 20643351). Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1 (PubMed : 28218735).

See full target information AURKA

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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:1555-1568 PubMed40034075

2025

Suppressive role of fukutin on cell proliferation in uterine cervical carcinoma in relation to Aurora-A kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoko Yamamoto,Yukinori Okamura,Yoichiro Kato,Kenta Masui,Yoji Nagasima,Atsushi Kurata

Cells 12: PubMed37887278

2023

Aurora Kinase A Regulates Cell Transitions in Glucocorticoid-Induced Bone Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Qiao,Yang Yang,Yan Zhao,Xiuju Wu,Li Zhang,Xinjiang Cai,Jaden Ji,Kristina I Boström,Yucheng Yao

Cell communication and signaling : CCS 21:156 PubMed37370099

2023

The crescent-like Golgi ribbon is shaped by the Ajuba/PRMT5/Aurora-A complex-modified HURP.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Chih Chiu,Xin-Ting Yang,Tong-You Wade Wei,Yu-Ting Amber Liao,Jo-Mei Maureen Chen,Yi-Chun Kuo,Chun-Chih Jared Liu,Chiao-Yun Cheng,Yu-Ting Jenny Huang,Yun-Ru Jaoying Huang,He-Lian Joe Wu,Chang-Xin Wan,Jia-Rung Tsai,Chang-Tze Ricky Yu

Cell & bioscience 12:195 PubMed36471438

2022

Aurora kinase a promotes the progression of papillary thyroid carcinoma by activating the mTORC2-AKT signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zewei Zhao,Huijuan Wang,Ning Kang,Zhongyu Wang,Xiukun Hou,Linfei Hu,Shuo Qie,Jianping Guo,Songfeng Wei,Xianhui Ruan,Xiangqian Zheng

Cancer cell international 22:277 PubMed36064409

2022

circ_0061265 competitively binds to microRNA-885-3p to promote the development of gastric cancer by upregulating AURKA expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Fei,Yuhe Lin,Mi Zhang,Jinshuai Guo,Yuan Liang

International journal of molecular sciences 23: PubMed35887149

2022

Body Hypomethylation Is Frequent in Tumors from the Digestive and Respiratory Tracts and Is Associated with Risk-Factor Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Diego Camuzi,Luisa Aguirre Buexm,Simone de Queiroz Chaves Lourenço,Rachele Grazziotin,Simone Guaraldi,Priscila Valverde,Davy Rapozo,Jill M Brooks,Hisham Mehanna,Luis Felipe Ribeiro Pinto,Sheila Coelho Soares-Lima

Molecular cancer research : MCR 20:1379-1390 PubMed35612556

2022

Dependence on the MUC1-C Oncoprotein in Classic, Variant, and Non-neuroendocrine Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Fushimi,Yoshihiro Morimoto,Satoshi Ishikawa,Nami Yamashita,Atrayee Bhattacharya,Tatsuaki Daimon,Hasan Rajabi,Caining Jin,Masayuki Hagiwara,Yota Yasumizu,Zhou Luan,Wenhao Suo,Kwok-Kin Wong,Henry Withers,Song Liu,Mark D Long,Donald Kufe

International journal of molecular sciences 23: PubMed35163790

2022

Novel Aurora A Kinase Inhibitor Fangchinoline Enhances Cisplatin-DNA Adducts and Cisplatin Therapeutic Efficacy in OVCAR-3 Ovarian Cancer Cells-Derived Xenograft Model.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Winardi,Pei-Yi Chu,Guan-Yu Chen,Ke Wang,Wei-Yu Hsu,Ching-Liang Hsieh,Yung-Hsiang Chen,Yang-Chang Wu,Juan-Cheng Yang

Histology and histopathology 37:397-404 PubMed34951005

2021

Targeting AURKA by microRNA-490-3p suppresses gastric cancer cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Chen,Shenkang Zhou,Chengfeng Fang,Feifei Ye,Jianhui Chen,Pinlu Jiang

Carcinogenesis 43:67-76 PubMed34657147

2021

MUC1-C dictates neuroendocrine lineage specification in pancreatic ductal adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Luan,Yoshihiro Morimoto,Atsushi Fushimi,Nami Yamashita,Wenhao Suo,Atrayee Bhattacharya,Masayuki Hagiwara,Caining Jin,Donald Kufe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Aurora A antibody - Centrosome Marker

Immunoprecipitation - Anti-Aurora A antibody - Centrosome Marker (AB1287)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (45)

Product promise