JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190374

Anti-Aurora A + B antibody [3H1]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal Aurora A antibody. Suitable for WB, ICC/IF and reacts with Human, Recombinant fragment samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AURKA.

View Alternative Names

AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6, AURKA, Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, Breast tumor-amplified kinase, Ipl1- and aurora-related kinase 1, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase Ayk1, Serine/threonine-protein kinase aurora-A, ARK-1, Aurora-related kinase 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Aurora A + B antibody [3H1] (AB190374)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aurora A + B antibody [3H1] (AB190374)

Immunofluorescent analysis of HeLa cells labeling Aurora A + B with ab190374 at 1/500 dilution (green). Cells were counterstained with a polyclonal antibody to Vimentin (red). Blue is a DNA stain.

Western blot - Anti-Aurora A + B antibody [3H1] (AB190374)
  • WB

Supplier Data

Western blot - Anti-Aurora A + B antibody [3H1] (AB190374)

All lanes:

Western blot - Anti-Aurora A + B antibody [3H1] (ab190374) at 1/1000 dilution

Lane 1:

HeLa cells treated with 100ng/ml nocodazole for 18 hours

Lane 2:

Recombinant Aurora A

Lane 3:

Recombinant Aurora B

Lane 4:

Recombinant Aurora C

Predicted band size: 38 kDa,46 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3H1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human AURKA.

O14965

Specificity

ab190374 was tested for binding to expressed human Aurora A, B and C and shown to react with both Aurora A and B, but not C.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aurora A and Aurora B are serine/threonine kinases also known as STK6 and STK12 respectively. They play a central role in cell division by controlling important processes like centrosome maturation and cytokinesis. Aurora A has a molecular mass of approximately 46 kDa while Aurora B is around 39 kDa. These proteins are expressed in proliferating tissues and have increased expression in cancer cells. Their expression patterns suggest a role in regulating cell cycle progression. The activity of Aurora A and B is cell cycle-dependent peaking during mitosis.
Biological function summary

Aurora kinases serve as regulators of mitotic events to ensure proper chromosome segregation. Aurora A is often associated with the centrosome and mitotic spindle while Aurora B part of the chromosomal passenger complex functions at the centromeres and midzone of dividing cells. They work together to execute processes such as spindle assembly and the correction of incorrect kinetochore-microtubule attachments. This coordination is essential for preserving genomic stability across cell divisions.

Pathways

Aurora A and B kinases function in the regulation of the mitotic checkpoint and spindle assembly pathway. They interact closely with proteins such as PLK1 and BUB1B which are involved in ensuring accurate chromosome alignment and separation. Aurora A's involvement in centrosome maturation links it to pathways regulating cell polarity while Aurora B's role in cytokinesis connects it to pathways overseeing cleavage plane determination and contractile ring formation. These pathways integrate signals from cell division regulators and communicate with other checkpoint proteins to coordinate orderly mitosis.

Dysregulation of Aurora kinases has a strong link to various cancers given their role in chromosome segregation and cell cycle control. Overexpression or mutations in Aurora A and B frequently occur in breast cancer and colorectal cancer contributing to oncogenic transformation and tumor progression. Their aberrant activity results in aneuploidy and mitotic defects often co-occurring with alterations in proteins like TP53 a well-known tumor suppressor. This relationship with cancer highlights the significance of Aurora kinases as potential therapeutic targets in oncology research and drug development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression (PubMed : 11039908, PubMed : 12390251, PubMed : 17125279, PubMed : 17360485, PubMed : 18615013, PubMed : 26246606). Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis (PubMed : 14523000, PubMed : 26246606). Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase (PubMed : 27335426). Required for initial activation of CDK1 at centrosomes (PubMed : 13678582, PubMed : 15128871). Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2 (PubMed : 11551964, PubMed : 14702041, PubMed : 15128871, PubMed : 15147269, PubMed : 15987997, PubMed : 17604723, PubMed : 18056443, PubMed : 18615013). Regulates KIF2A tubulin depolymerase activity (PubMed : 19351716). Important for microtubule formation and/or stabilization (PubMed : 18056443). Required for normal axon formation (PubMed : 19812038). Plays a role in microtubule remodeling during neurite extension (PubMed : 19668197). Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53 (PubMed : 14702041). Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity (PubMed : 11551964). Inhibits cilia outgrowth (By similarity). Required for cilia disassembly via phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed : 17604723, PubMed : 20643351). Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1 (PubMed : 28218735).
See full target information AURKA

Additional targets

AURKB
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39147589

2024

Heterogeneity in Slow Synaptic Transmission Diversifies Purkinje Cell Timing.

Applications

Unspecified application

Species

Unspecified reactive species

Riya Elizabeth Thomas,Franziska Mudlaff,Kyra Schweers,William Todd Farmer,Aparna Suvrathan
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB17056

Anti-PLK1 antibody [35-206]

Trial Size

Immunocytochemistry - Anti-PLK1 antibody [35-206] (AB17056)

  • 4
  • 6
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB2254

Anti-Aurora B antibody

BOND RX™ Validated

Western blot - Anti-Aurora B antibody (AB2254)

  • 4
  • 36

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com