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Anti-Aurora B antibody ab2254 is a rabbit polyclonal antibody that is used in Aurora B western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Tried and trusted by researchers since 2003


Images

Western blot - Anti-Aurora B antibody (AB2254), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (AB2254), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (AB2254), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (AB2254), expandable thumbnail
  • Western blot - Anti-Aurora B antibody (AB2254), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Hamster
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

0.5-1 µg/mL

Notes

Methanol fixation recommended.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Hamster, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/2000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/2000

Notes

-

Species

Human

Dilution info

1/1000 - 1/2000

Notes

-

Predicted
Predicted

Species

Hamster, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Hamster, Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis (PubMed:11516652, PubMed:12925766, PubMed:14610074, PubMed:14722118, PubMed:29449677). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly (PubMed:11516652, PubMed:12925766, PubMed:14610074, PubMed:14722118, PubMed:26829474). Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis (PubMed:15249581). Required for central/midzone spindle assembly and cleavage furrow formation (PubMed:12458200, PubMed:12686604). Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP (PubMed:11516652, PubMed:12925766, PubMed:14610074). Phosphorylation of INCENP leads to increased AURKB activity (PubMed:11516652, PubMed:12925766, PubMed:14610074). Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/vimentin, HASPIN, and histone H3 (PubMed:11756469, PubMed:11784863, PubMed:11856369, PubMed:12689593, PubMed:14602875, PubMed:16103226, PubMed:21658950). A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres (PubMed:21658950). Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively) (PubMed:11784863, PubMed:11856369). AURKB is also required for kinetochore localization of BUB1 and SGO1 (PubMed:15020684, PubMed:17617734). Phosphorylation of p53/TP53 negatively regulates its transcriptional activity (PubMed:20959462). Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes (By similarity). Acts as an inhibitor of CGAS during mitosis: catalyzes phosphorylation of the N-terminus of CGAS during the G2-M transition, blocking CGAS liquid phase separation and activation, and thereby preventing CGAS-induced autoimmunity (PubMed:33542149). Phosphorylates KRT5 during anaphase and telophase (By similarity). Phosphorylates ATXN10 which promotes phosphorylation of ATXN10 by PLK1 and may play a role in the regulation of cytokinesis and stimulating the proteasomal degradation of ATXN10 (PubMed:25666058).

Alternative names

Recommended products

Anti-Aurora B antibody ab2254 is a rabbit polyclonal antibody that is used in Aurora B western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Tried and trusted by researchers since 2003

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aurora B also known as Aurora kinase B or AURKB is a serine/threonine protein kinase with a molecular mass of approximately 39 kDa. This protein expresses across various cell types and tissues playing a critical role during cell division. It localizes to the centromeres during early mitosis and later associates with the central spindle and midbody. Aurora B is a component of the chromosomal passenger complex (CPC) which includes other proteins such as INCENP survivin and borealin essential for its function.

Biological function summary

Aurora B kinase functions to ensure proper chromosome alignment segregation and cytokinesis during mitosis and meiosis. It phosphorylates various substrates to facilitate processes like correction of kinetochore-microtubule attachments and regulation of the mitotic checkpoint. As a part of the CPC Aurora B acts in coordination with other proteins to control these cellular events ensuring that cells divide accurately and safely.

Pathways

Aurora B integrates into the cell cycle and mitotic pathways operating closely with the spindle assembly checkpoint. It interacts with proteins like CENP-A and CENP-E ensuring error-free chromosome segregation. Aurora B's activity within these pathways is critical for maintaining genomic stability and preventing aneuploidy a condition linked to improper chromosome number.

Associated diseases and disorders

Aurora B's malfunction associates with cancer and chromosomal instability. Overexpression or dysregulation of Aurora B kinase frequently occurs in several types of cancer contributing to tumorigenesis by promoting abnormal cell division. Proteins such as p53 a well-known tumor suppressor often become functionally compromised when Aurora B activity is altered further advancing disease progression. Aurora B also holds implications in other disorders marked by faulty cell cycle regulation emphasizing its potential as a therapeutic target.

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11 product images

  • Western blot - Anti-Aurora B antibody (ab2254), expandable thumbnail
    Balboula and Schindler PLoS Genet. 2014 Feb 27;10(2):e1004194. doi: 10.1371/journal.pgen.1004194. eCollection 2014 Feb. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Western blot - Anti-Aurora B antibody (ab2254)

    AURKB is expressed in mouse oocytes.

    (Panel D) 20 GV-intact oocytes were collected from CF1 mice and micro-injected with the indicated cRNA. Two hours after injection, the oocytes were matured to Met II in vitro (16 h). The total numbers of non-injected control oocytes (Non-inj.) are indicated in parenthesis. Total cellular lysates were probed with the indicated antibody. The panels are images of the same membrane that was stripped and re-probed. The arrows indicate the specific AURKB protein band, and the asterisk indicates a presumed degradation product of AURKB-GFP.

    All lanes: Western blot - Anti-Aurora B antibody (ab2254)

    Predicted band size: 39 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254), expandable thumbnail
    Balboula and Schindler PLoS Genet. 2014 Feb 27;10(2):e1004194. doi: 10.1371/journal.pgen.1004194. eCollection 2014 Feb. Fig 1.

    Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)

    AURKB is expressed in mouse oocytes.

    (Panel A) GV-intact oocytes were collected from CF1 mice and matured in vitro for 8 h (Met I), or 16 h (Met II), prior to fixation and staining with an anti-AURKB antibody (ab2254).

  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)

    ab2254 stained in Hela cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2254 at 1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (pseudo-colored red) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43μM for 1hour at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254), expandable thumbnail
    Chopra et al PLoS One. 2016 Apr 20;11(4):e0153818. doi: 10.1371/journal.pone.0153818. eCollection 2016. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)

    HCT 116 (Human colorectal carcinoma cell line) cells were examined by immunofluorescence confocal microscopy.

    Cells were treated with 500 nM AK301 for 16 hours, and then processed for Aurora B (ab2254) and β-tubulin staining (Panel B). The color key and 20 μm bars are shown. Images of representative field is shown with a 20 μm bar. End-labeled DNA is shown in red and DAPI-stained DNA is blue.

    Cells cultured on coverslips were fixed with 4% paraformaldehyde at room temperature or 100% ice cold methanol at 4°C and then permeabilized with 0.5% Triton X-100 in PBS. Cells were blocked in 5% serum (in PBS) and then incubated with primary antibody (in 5% serum) on shaker for 1 h at room temperature.

  • Western blot - Anti-Aurora B antibody (ab2254), expandable thumbnail

    Western blot - Anti-Aurora B antibody (ab2254)

    Blocked with 2% BSA.

    All lanes: Western blot - Anti-Aurora B antibody (ab2254) at 1 µg/mL

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: HeLa nocodozole treated cell lysate at 10 µg

    Lane 3: NIH3T3 cell lysate at 10 µg

    Lane 4: PC12 cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Performed under reducing conditions.

    Predicted band size: 39 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)

    Immunofluorescence in human cells using Rabbit polyclonal to Aurora B (red), DAPI (blue) and CREST serum (binds to centromeres)(green).

    (a) HeLa cells - transition from interphase (left) through mitosis
    (b) RPE-1 cells - as in (a)
    (c) HeLa cells - interphase
    (d) RPE-1 cells - interphase

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody (ab2254), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody (ab2254)

    IHC image of Aurora B staining in Human Lymph node Hodgkins disease formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2254, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254), expandable thumbnail
    This image is courtesy of a customer review from Lux Fatimathas.

    Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)

    ab2254 staining human A431 (epithelial) cells by ICC/IF. The sample was fixed in paraformaldehyde and permeabilized by incubation with 0.1% Triton X100. 1% BSA was used as the blocking agent prior to a 1 hour incubation with the primary antibody, diluted 1/1000 with 1% BSA made up in PBS. An Alexa Fluor® 647 conjugated Donkey anti-Rabbit IgG (H+L) antibody was used as the secondary. Blocking and antibody incubation steps were carried out at room temperature.

    In this set of images, the tubulin is stained green, Aurora B in pink and DNA in blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody (ab2254), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody (ab2254)

    Rabbit polyclonal to Aurora B (ab2254) used to stain SW620 human tumour xenografts (in mouse).

    The sections were microwave pretreated in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). Slides were then incubated for 1 hour with the Aurora B primary antibody diluted 1/200 in TBS, then visualised using DAB, after application of an appropriate secondary.

  • Western blot - Anti-Aurora B antibody (ab2254), expandable thumbnail

    Western blot - Anti-Aurora B antibody (ab2254)

    All lanes: Western blot - Anti-Aurora B antibody (ab2254) at 1 µg/mL

    Lane 1: HeLa Whole Cell Lysate at 20 µg

    Lane 2: HeLa Nuclear Lysate at 20 µg

    Lane 3: Jurkat Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa

    Observed band size: 37 kDa, 39 kDa

    Exposure time: 150s

  • Western blot - Anti-Aurora B antibody (ab2254), expandable thumbnail

    Western blot - Anti-Aurora B antibody (ab2254)

    All lanes: Western blot - Anti-Aurora B antibody (ab2254) at 1/2000 dilution

    All lanes: Western blot - Recombinant human Aurora B protein (Recombinant human Aurora B protein ab51435) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa

    Exposure time: 30s

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Product protocols

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