Skip to main content

Rabbit Recombinant Monoclonal Aurora B phospho S227 antibody. Suitable for IP, Dot, WB, ICC/IF and reacts with Human, Synthetic peptide samples. Cited in 1 publication.


Images

Immunoprecipitation - Anti-Aurora B (phospho S227) antibody [EPR20389] (AB210706), expandable thumbnail
  • Western blot - Anti-Aurora B (phospho S227) antibody [EPR20389] (AB210706), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B (phospho S227) antibody [EPR20389] (AB210706), expandable thumbnail
  • Dot Blot - Anti-Aurora B (phospho S227) antibody [EPR20389] (AB210706), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWBICC/IF
Human
Tested
Expected
Tested
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.

Alternative names

Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B, AIM-1, ARK-2, Aurora-related kinase 2, STK12, AURKB, AIK2, AIM1, AIRK2, STK5, STK1, ARK2

Recommended products

Rabbit Recombinant Monoclonal Aurora B phospho S227 antibody. Suitable for IP, Dot, WB, ICC/IF and reacts with Human, Synthetic peptide samples. Cited in 1 publication.

Alternative names

Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B, AIM-1, ARK-2, Aurora-related kinase 2, STK12, AURKB, AIK2, AIM1, AIRK2, STK5, STK1, ARK2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20389

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aurora B also known as Aurora kinase B or AURKB is a serine/threonine protein kinase with a molecular mass of approximately 39 kDa. This protein expresses across various cell types and tissues playing a critical role during cell division. It localizes to the centromeres during early mitosis and later associates with the central spindle and midbody. Aurora B is a component of the chromosomal passenger complex (CPC) which includes other proteins such as INCENP survivin and borealin essential for its function.

Biological function summary

Aurora B kinase functions to ensure proper chromosome alignment segregation and cytokinesis during mitosis and meiosis. It phosphorylates various substrates to facilitate processes like correction of kinetochore-microtubule attachments and regulation of the mitotic checkpoint. As a part of the CPC Aurora B acts in coordination with other proteins to control these cellular events ensuring that cells divide accurately and safely.

Pathways

Aurora B integrates into the cell cycle and mitotic pathways operating closely with the spindle assembly checkpoint. It interacts with proteins like CENP-A and CENP-E ensuring error-free chromosome segregation. Aurora B's activity within these pathways is critical for maintaining genomic stability and preventing aneuploidy a condition linked to improper chromosome number.

Associated diseases and disorders

Aurora B's malfunction associates with cancer and chromosomal instability. Overexpression or dysregulation of Aurora B kinase frequently occurs in several types of cancer contributing to tumorigenesis by promoting abnormal cell division. Proteins such as p53 a well-known tumor suppressor often become functionally compromised when Aurora B activity is altered further advancing disease progression. Aurora B also holds implications in other disorders marked by faulty cell cycle regulation emphasizing its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706), expandable thumbnail

    Immunoprecipitation - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

    Aurora B (phospho S227) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole for 18 hours whole cell lysate with ab210706 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210706 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate 10μg (Input).

    Lane 2: ab210706 IP in HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab210706 in HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds

    All lanes: Immunoprecipitation - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

    Predicted band size: 39 kDa

  • Western blot - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706), expandable thumbnail

    Western blot - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706) at 1/1000 dilution

    Lane 1: Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa

    Exposure time: 3min

    This data was developed using ab210706, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Aurora B (phospho S227) with ab210706 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing midbody staining (arrow) on HeLa cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Dot Blot - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706), expandable thumbnail

    Dot Blot - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

    Dot blot analysis of Aurora B (phospho S227) labeled with ab210706 at 1/1000 dilution.

    Lane 1: Aurora B (phospho S227) peptide.

    Lane 2: Aurora B non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com