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AB243042

Anti-AUTS2 antibody [CL7067]

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(1 Publication)

Mouse Monoclonal AUTS2 antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human AUTS2 aa 1050-200.

View Alternative Names

KIAA0442, AUTS2, Autism susceptibility gene 2 protein

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-AUTS2 antibody [CL7067] (AB243042)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AUTS2 antibody [CL7067] (AB243042)

Paraffin-embedded, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling AUTS2 (green) using ab243042 at 10 μg/mL in ICC/IF analysis. Microtubules visulized in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7067] (AB243042)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7067] (AB243042)

Paraffin-embedded mouse embryo E14 tissue stained for AUTS2 using ab243042 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7067] (AB243042)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7067] (AB243042)

Paraffin-embedded mouse embryo E14 tissue stained for AUTS2 using ab243042 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7067] (AB243042)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7067] (AB243042)

Paraffin-embedded mouse cerebellum tissue stained for AUTS2 using ab243042 at 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL7067

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human AUTS2 aa 1050-200. The exact immunogen used to generate this antibody is proprietary information.

Q8WXX7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AUTS2 also known as the Autism Susceptibility Gene 2 is a protein encoded by the AUTS2 gene and has a mass of approximately 141 kDa. This protein is expressed in several regions of the brain including the cerebral cortex hippocampus and cerebellum. It plays a role in neuronal development where it impacts neural progenitor cell proliferation and differentiation. Expression of AUTS2 is often regulated during key neurodevelopmental stages.
Biological function summary

The AUTS2 protein is involved in chromatin remodeling impacting gene expression regulation during brain development. It functions as part of a complex involving other transcription factors and regulators to influence diverse neural processes. This involvement in chromatin remodeling highlights its role in controlling when and how genes are activated within cells impacting neurodevelopmental processes significantly.

Pathways

AUTS2 participates in the regulation of neuronal signaling pathways such as the Wnt signaling pathway which is key for cell fate determination during development. Proteins like TCF/LEF are related to AUTS2 through the Wnt signaling pathway where they serve as transcriptional mediators affected by the pathway's activity. This connection indicates AUTS2's role in maintaining the balance between progenitor cell proliferation and differentiation in neural tissues.

AUTS2 has a significant association with autism spectrum disorders (ASD) and intellectual disability. Disruptions or mutations in the AUTS2 gene often associate with these neurodevelopmental conditions. The protein also has connections with other proteins like CHD8 which is often mutated in autism suggesting that alterations affecting AUTS2 could influence broader functional networks in the brain responsible for cognitive development and behavior.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 25519132). The PRC1-like complex that contains PCGF5, RNF2, CSNK2B, RYBP and AUTS2 has decreased histone H2A ubiquitination activity, due to the phosphorylation of RNF2 by CSNK2B (PubMed : 25519132). As a consequence, the complex mediates transcriptional activation (PubMed : 25519132). In the cytoplasm, plays a role in axon and dendrite elongation and in neuronal migration during embryonic brain development. Promotes reorganization of the actin cytoskeleton, lamellipodia formation and neurite elongation via its interaction with RAC guanine nucleotide exchange factors, which then leads to the activation of RAC1 (By similarity).
See full target information AUTS2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 32:447-465 PubMed39402212

2024

SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Wang,Ting He,Ya Luo,Kexin Ren,Huanming Shen,Lingfeng Hou,Yixin Wei,Tong Fu,Wenlong Xie,Peng Wang,Jie Hu,Yu Zhu,Zhengrong Huang,Qiyuan Li,Weihua Li,Huiling Guo,Boan Li
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Product promise

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