AB243036

Anti-AUTS2 antibody [CL7084]

Name: Anti-AUTS2 antibody [CL7084] (ab243036) | Abcam
Brand: Abcam Limited
SKU: AB243036
Price: 630 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal AUTS2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human AUTS2 aa 1050-1100.

View Alternative Names

KIAA0442, AUTS2, Autism susceptibility gene 2 protein

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Paraffin-embedded mouse cerebellum tissue stained for AUTS2 using ab243036 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Paraffin-embedded mouse hippocampus tissue stained for AUTS2 using ab243036 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Paraffin-embedded mouse embryo E14 tissue stained for AUTS2 using ab243036 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Paraffin-embedded mouse embryo E14 tissue stained for AUTS2 using ab243036 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-AUTS2 antibody [CL7084] (AB243036)

All lanes:

Western blot - Anti-AUTS2 antibody [CL7084] (ab243036) at 1 µg/mL

All lanes:

SCLC-21H cell lysate

Predicted band size: 139 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL7084

Isotype

IgG2a

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human AUTS2 aa 1050-1100. The exact immunogen used to generate this antibody is proprietary information.

Q8WXX7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AUTS2 also known as the Autism Susceptibility Gene 2 is a protein encoded by the AUTS2 gene and has a mass of approximately 141 kDa. This protein is expressed in several regions of the brain including the cerebral cortex hippocampus and cerebellum. It plays a role in neuronal development where it impacts neural progenitor cell proliferation and differentiation. Expression of AUTS2 is often regulated during key neurodevelopmental stages.

Biological function summary

The AUTS2 protein is involved in chromatin remodeling impacting gene expression regulation during brain development. It functions as part of a complex involving other transcription factors and regulators to influence diverse neural processes. This involvement in chromatin remodeling highlights its role in controlling when and how genes are activated within cells impacting neurodevelopmental processes significantly.

Pathways

AUTS2 participates in the regulation of neuronal signaling pathways such as the Wnt signaling pathway which is key for cell fate determination during development. Proteins like TCF/LEF are related to AUTS2 through the Wnt signaling pathway where they serve as transcriptional mediators affected by the pathway's activity. This connection indicates AUTS2's role in maintaining the balance between progenitor cell proliferation and differentiation in neural tissues.

AUTS2 has a significant association with autism spectrum disorders (ASD) and intellectual disability. Disruptions or mutations in the AUTS2 gene often associate with these neurodevelopmental conditions. The protein also has connections with other proteins like CHD8 which is often mutated in autism suggesting that alterations affecting AUTS2 could influence broader functional networks in the brain responsible for cognitive development and behavior.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 25519132). The PRC1-like complex that contains PCGF5, RNF2, CSNK2B, RYBP and AUTS2 has decreased histone H2A ubiquitination activity, due to the phosphorylation of RNF2 by CSNK2B (PubMed : 25519132). As a consequence, the complex mediates transcriptional activation (PubMed : 25519132). In the cytoplasm, plays a role in axon and dendrite elongation and in neuronal migration during embryonic brain development. Promotes reorganization of the actin cytoskeleton, lamellipodia formation and neurite elongation via its interaction with RAC guanine nucleotide exchange factors, which then leads to the activation of RAC1 (By similarity).

See full target information AUTS2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 150:23 PubMed40906043

2025

TDP-43 dysregulation impairs cholesterol metabolism linked with myelination defects.

Applications

Unspecified application

Species

Unspecified reactive species

Irene García-Toledo,Juan M Godoy-Corchuelo,Luis C Fernández-Beltrán,Zeinab Ali,Ariadna Guindo-Arroyo,Irene Jiménez-Coca,Jesús Jiménez-Rodríguez,Karen Javaloyes-García,Marcos Viñuela,Ulises Gómez-Pinedo,Laura Saiz-Aúz,Alberto Rábano,Estela Área-Gómez,Thomas J Cunningham,Silvia Corrochano

Nature 598:205-213 PubMed34616060

2021

Single-cell epigenomics reveals mechanisms of human cortical development.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan S Ziffra,Chang N Kim,Jayden M Ross,Amy Wilfert,Tychele N Turner,Maximilian Haeussler,Alex M Casella,Pawel F Przytycki,Kathleen C Keough,David Shin,Derek Bogdanoff,Anat Kreimer,Katherine S Pollard,Seth A Ament,Evan E Eichler,Nadav Ahituv,Tomasz J Nowakowski

Nature 578:142-148 PubMed31996853

2020

Cell stress in cortical organoids impairs molecular subtype specification.

Applications

Unspecified application

Species

Unspecified reactive species

Aparna Bhaduri,Madeline G Andrews,Walter Mancia Leon,Diane Jung,David Shin,Denise Allen,Dana Jung,Galina Schmunk,Maximilian Haeussler,Jahan Salma,Alex A Pollen,Tomasz J Nowakowski,Arnold R Kriegstein

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AUTS2 antibody [CL7084]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AUTS2 antibody [CL7084] (AB243036)

Western blot - Anti-AUTS2 antibody [CL7084] (AB243036)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise