AB327586

Anti-Avian Influenza A virus H5N1 NA (Neuraminidase) antibody [HL4724] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal NRAM antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Influenza A virus (A/Viet Nam/1203/2004(H5N1)) samples.

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Supplier Data

Western blot - Anti-Avian Influenza A virus H5N1 NA (Neuraminidase) antibody [HL4724] - BSA and Azide free (AB327586)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327586 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Avian Influenza A virus H5N1 NA (Neuraminidase) antibody [HL4724] - BSA and Azide free (ab327586) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extract at 30 µg

Lane 2:

DDDDK-tagged flu A_NA (A/Viet Nam/1203/2004/H5N1) transfected 293T whole cell extract at 30 µg

Lane 3:

DDDDK-tagged flu A_NA (A/WSN/1933/H1N1) transfected 293T whole cell extract at 30 µg

Lane 4:

DDDDK-tagged flu A_NA (A/Jiangsu/428/2021/H10/N3) transfected 293T whole cell extract at 30 µg

Lane 5:

DDDDK-tagged flu A_NA (A/Astrakhan/3212/2020/H5N8) transfected 293T whole cell extract at 30 µg

Lane 6:

DDDDK-tagged flu B_NA (B/Taiwan/753/2005) transfected 293T whole cell extract at 30 µg

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL4724

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A virus (A/Viet Nam/1203/2004(H5N1))

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody is specific for Avian Influenza A virus H5N1 NA protein, and it does not cross-react with NA protein of Influenza A virus H1N1, Avian Influenza A virus H5N8 and H10N3, and Influenza B virus.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A virus (A/Viet Nam/1203/2004(H5N1))": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Influenza A virus (A/Viet Nam/1203/2004(H5N1))": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.

See full target information NA

Alternative Names

Neuraminidase

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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