AB308170

Anti-Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550]

Recombinant
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Rabbit Recombinant Monoclonal Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody. Carrier free. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Influenza A, Transfected cell line - Influenza A samples.

View Alternative Names

HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, Hemagglutinin precursor

2 Images

Immunohistochemistry paraffin embedded sections - Anti-Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (AB308170)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (AB308170)

Immunohistochemical analysis of paraffin-embedded mock and Avian Influenza A virus H5N8 HA - transfected 293T cell pellets labeling Avian Influenza A virus H5N8 HA (Hemagglutinin) protein with ab308170 at 1/1000 dilution (green). Cytoplasm staining on HEK-293T is observed. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins. Nuclear DNA was labeled with Fluoroshield with DAPI (blue).

Supplier Data

Western blot - Anti-Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (AB308170)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (ab308170) diluted at 1 : 100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (ab308170) at 1/100000 dilution

Lane 1:

Non-transfected (–) HEK-293T whole cell lysate at 30 µg

Lane 2:

Transfected (+) HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 64 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1550

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A

Applications

WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell line - Influenza A": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate - Influenza A": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Avian Influenza A Hemagglutinin (HA) also known as influenza hemagglutinin is a surface glycoprotein with a molecular mass of approximately 63 kilodaltons. HA is expressed on the envelope of the influenza virus where it facilitates viral entry into host cells. This protein binds to sialic acid receptors on the surface of host cells initiating endocytosis and subsequent viral internalization. HA is a critical determinant of host range and antigenicity in avian influenza viruses.

Biological function summary

The avian protein HA plays a central role in the infection cycle of influenza viruses. As part of the viral capsid hemagglutinin undergoes a conformational change upon receptor binding triggering membrane fusion and release of viral RNA into the host cytoplasm. HA is integral to the structure of the virus working alongside neuraminidase which facilitates release of new viral particles. The interaction between hemagglutinin and host receptor is an important step in viral infectivity and immune system evasion.

Pathways

Hemagglutinin interacts closely with cellular and viral pathways influencing viral replication and assembly. HA operates in conjunction with the neuraminidase protein in the viral life cycle particularly in the replication of the genetic material and assembly of the virion. During the entry pathway the low-pH environment in endosomes prompts a change in HA structure leading to fusion of viral and endosomal membranes an important event for viral genome release into the host cytoplasm.

HA of avian influenza is strongly linked to viral pathogenicity and cross-species transmission. Avian influenza A particularly subtypes like H5N1 can cause severe respiratory diseases in birds and humans with HA being an important virulence factor. Mutations in hemagglutinin can alter viral transmissibility and immune escape impacting disease severity. HA also has potential connections with other influenza-related proteins such as matrix protein 2 (M2) which jointly determine viral pathogenicity and host immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Avian Influenza A virus H5N8 HA (Hemagglutinin)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550]

Immunohistochemistry paraffin embedded sections - Anti-Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (AB308170)

Western blot - Anti-Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (AB308170)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise