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AB22285

Anti-Avian Influenza Nucleoprotein antibody

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(4 Publications)

Rabbit Polyclonal NCAP antibody. Suitable for ELISA, ICC/IF and reacts with Influenza A samples. Cited in 4 publications.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N, NP

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Avian Influenza Nucleoprotein antibody (AB22285)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Avian Influenza Nucleoprotein antibody (AB22285)

Immunofluoroscence staining of influenza−infected MDCK cells using ab22285 at 1 : 10 dilution.

Image Courtesy of Catherine Thompson, The University of Reading

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Influenza A

Applications

ICC/IF, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "0.1-1 µg/mL", "ELISA-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Avian Influenza Nucleoprotein also known as NP is a structural protein found in influenza viruses particularly avian strains. It functions mechanically as a part of the ribonucleoprotein (RNP) complex encapsulating the viral RNA in a helical structure therefore facilitating its replication and transcription. The molecular weight of the nucleoprotein is approximately 56 kDa. It expresses predominantly within the host cell's cytoplasm participating in viral assembly and eventually packaging.
Biological function summary

The nucleoprotein functions as an important player in viral replication and interaction with host cell machinery. Within the RNP complex it binds to viral RNA and polymerase subunits ensuring the stability of viral genome replication. Additionally the nucleoprotein interacts with host nuclear import and export pathways to shuttle viral components across the nuclear membrane efficiently important for viral propagation within host cells.

Pathways

Avian Influenza Nucleoprotein influences viral RNA synthesis and export pathways. It associates with viral polymerase proteins such as PA PB1 and PB2 during the viral RNA replication process. Moreover its interaction with nuclear export factors like exportin-1 (CRM1) integrates it into the host's nuclear export pathway facilitating the movement of viral RNA from nucleus to cytoplasm which is essential for the completion of the viral life cycle.

Avian Influenza Nucleoprotein is central to the pathogenicity of avian influenza infections which can result in avian influenza virus (AIV) outbreaks. This relationship with AIV highlights its importance in zoonotic transmission and potential emergence of avian influenza in humans. Its association with proteins involved in the immune response such as interferon-inducible proteins also highlights its role in modulating host immune evasion a factor that complicates the control of influenza outbreaks.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
See full target information NP
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 11:581867 PubMed33101257

2020

Influenza A Virus Nucleoprotein Activates the JNK Stress-Signaling Pathway for Viral Replication by Sequestering Host Filamin A Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Anshika Sharma,Jyoti Batra,Olga Stuchlik,Matthew S Reed,Jan Pohl,Vincent T K Chow,Suryaprakash Sambhara,Sunil K Lal

Cell death & disease 4:e562 PubMed23538443

2013

Influenza A virus nucleoprotein induces apoptosis in human airway epithelial cells: implications of a novel interaction between nucleoprotein and host protein Clusterin.

Applications

WB

Species

Unspecified reactive species

S Tripathi,J Batra,W Cao,K Sharma,J R Patel,P Ranjan,A Kumar,J M Katz,N J Cox,R B Lal,S Sambhara,S K Lal

Emerging infectious diseases 14:1671-2 PubMed18826847

2008

Detecting avian influenza virus (H5N1) in domestic duck feathers.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yamamoto,Kikuyasu Nakamura,Masatoshi Okamatsu,Ayako Miyazaki,Manabu Yamada,Masaji Mase

Emerging infectious diseases 14:1672-4 PubMed18826848

2008

Neisseria gonorrhoeae meningitis in pregnant adolescent.

Applications

Unspecified application

Species

Unspecified reactive species

Maria C Martín,Francisco Pérez,Alfonso Moreno,Alberto Moral,Miguel A Alvarez,Francisco J Méndez,Fernando Vázquez
View all publications
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Product promise

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