Rabbit Polyclonal NCAP antibody. Suitable for ELISA, ICC/IF and reacts with Influenza A samples. Cited in 4 publications.
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Publications
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- Frontiers in microbiology 11:5818672020Influenza A Virus Nucleoprotein Activates the JNK Stress-Signaling Pathway for Viral Replication by Sequestering Host Filamin A Protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnshika Sharma et. al.PubMed 33101257
- Cell death & disease 4:e5622013Influenza A virus nucleoprotein induces apoptosis in human airway epithelial cells: implications of a novel interaction between nucleoprotein and host protein Clusterin.
- Emerging infectious diseases 14:1671-22008Detecting avian influenza virus (H5N1) in domestic duck feathers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Yamamoto,Kikuyasu Nakamura,Masatoshi Okamatsu,Ayako Miyazaki,Manabu Yamada,Masaji MasePubMed 18826847
- Emerging infectious diseases 14:1672-42008Neisseria gonorrhoeae meningitis in pregnant adolescent.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria C Martín,Francisco Pérez,Alfonso Moreno,Alberto Moral,Miguel A Alvarez,Francisco J Méndez,Fernando VázquezPubMed 18826848
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| ELISA | ICC/IF | |
|---|---|---|
| Influenza A | Expected | Tested |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A | Dilution info 0.10000-1.00000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A | Dilution info 1/10 | Notes - |
Associated Products
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Target data
Function
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
Alternative names
Nucleoprotein, Nucleocapsid protein, Protein N, NP
Recommended products
Rabbit Polyclonal NCAP antibody. Suitable for ELISA, ICC/IF and reacts with Influenza A samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Avian Influenza Nucleoprotein also known as NP is a structural protein found in influenza viruses particularly avian strains. It functions mechanically as a part of the ribonucleoprotein (RNP) complex encapsulating the viral RNA in a helical structure therefore facilitating its replication and transcription. The molecular weight of the nucleoprotein is approximately 56 kDa. It expresses predominantly within the host cell's cytoplasm participating in viral assembly and eventually packaging.
- Biological function summary
The nucleoprotein functions as an important player in viral replication and interaction with host cell machinery. Within the RNP complex it binds to viral RNA and polymerase subunits ensuring the stability of viral genome replication. Additionally the nucleoprotein interacts with host nuclear import and export pathways to shuttle viral components across the nuclear membrane efficiently important for viral propagation within host cells.
- Pathways
Avian Influenza Nucleoprotein influences viral RNA synthesis and export pathways. It associates with viral polymerase proteins such as PA PB1 and PB2 during the viral RNA replication process. Moreover its interaction with nuclear export factors like exportin-1 (CRM1) integrates it into the host's nuclear export pathway facilitating the movement of viral RNA from nucleus to cytoplasm which is essential for the completion of the viral life cycle.
- Associated diseases and disorders
Avian Influenza Nucleoprotein is central to the pathogenicity of avian influenza infections which can result in avian influenza virus (AIV) outbreaks. This relationship with AIV highlights its importance in zoonotic transmission and potential emergence of avian influenza in humans. Its association with proteins involved in the immune response such as interferon-inducible proteins also highlights its role in modulating host immune evasion a factor that complicates the control of influenza outbreaks.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Image Courtesy of Catherine Thompson, The University of ReadingImmunocytochemistry/ Immunofluorescence - Anti-Avian Influenza Nucleoprotein antibody (ab22285)
Immunofluoroscence staining of influenza−infected MDCK cells using ab22285 at 1:10 dilution.
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