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AB250256

Anti-AWP1 antibody [EPR12323] - BSA and Azide free

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Rabbit Recombinant Monoclonal AWP1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

AWP1, ZA20D3, HT032, ZFAND6, AN1-type zinc finger protein 6, Associated with PRK1 protein, Zinc finger A20 domain-containing protein 3

2 Images
Western blot - Anti-AWP1 antibody [EPR12323] - BSA and Azide free (AB250256)
  • WB

Supplier Data

Western blot - Anti-AWP1 antibody [EPR12323] - BSA and Azide free (AB250256)

This data was developed using ab180856, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AWP1 antibody [EPR12323] (<a href='/en-us/products/primary-antibodies/awp1-antibody-epr12323-ab180856'>ab180856</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A673 cell lysate at 10 µg

Predicted band size: 22 kDa

Observed band size: 26 kDa

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Flow Cytometry (Intracellular) - Anti-AWP1 antibody [EPR12323] - BSA and Azide free (AB250256)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AWP1 antibody [EPR12323] - BSA and Azide free (AB250256)

This data was developed using ab180856, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of AWP1 expression in permeabilized Jurkat cells using ab180856 at 1/10 dilution (red) and a rabbit IgG as negative control (green).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12323

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250256 is the carrier-free version of ab180856.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AWP1 also known as AWP1 or AWP1 protein functions mechanically as an adapter molecule involved in various cellular processes. This protein is approximately 36 kDa in mass. AWP1 is expressed throughout many tissues including the heart liver and kidney. Its expression reflects the diverse roles it plays across different cellular environments where it modulates interactions between proteins.
Biological function summary

AWP1 participates in the regulation of signaling pathways and cellular communication. It acts as part of a protein complex that facilitates the interaction between membrane proteins and cytosolic components. AWP1's role in modulating these interactions is important for maintaining normal cellular activities impacting processes such as cell proliferation and apoptosis.

Pathways

AWP1 plays roles in the signaling and immune pathways. It influences the NF-kB signaling pathway an important pathway involved in immune response and inflammation. AWP1 interacts with proteins like TRAF6 impacting the cascade of signaling events that regulate cellular responses to external stimuli. Additionally it participates in pathways related to cellular stress responses integrating signals that dictate cell survival.

AWP1 associates with conditions like inflammation and cancer. In certain cancers dysregulated AWP1 expression is connected to abnormal cell proliferation and survival. The protein interacts with other key players like PI3K which contribute to oncogenic processes in cancer. In inflammatory disorders AWP1's role in modulating NF-kB signaling can lead to altered immune responses potentially exacerbating disease severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in regulation of TNF-alpha induced NF-kappa-B activation and apoptosis. Involved in modulation of 'Lys-48'-linked polyubiquitination status of TRAF2 and decreases association of TRAF2 with RIPK1. Required for PTS1 target sequence-dependent protein import into peroxisomes and PEX5 stability; may cooperate with PEX6. In vitro involved in PEX5 export from the cytosol to peroxisomes (By similarity).
See full target information ZFAND6
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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