AB115205

Anti-Axin 1 antibody

Name: Anti-Axin 1 antibody (ab115205) | Abcam
Brand: Abcam Limited
SKU: AB115205
Rating: 4 (1 reviews)

(1 Review)

(5 Publications)

Goat Polyclonal Axin 1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human AXIN1 aa 850 to C-terminus.

View Alternative Names

AXIN, AXIN1, Axin-1, Axis inhibition protein 1, hAxin

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (AB115205)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (AB115205)

ab114205, at 3.75 ug/ml, staining Axin-1 in formalin-fixed, paraffin-embedded Human adrenal tissue by immunohistochemistry.

IHC-P

AbReview32513****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (AB115205)

Immunohistochemical analysis of rat bladder tissue, staining Axin 1 with ab115205.

Tissue was fixed with formalin and blocked with 5000 μg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/400) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human AXIN1 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15169

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Baboon": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Axin 1 alternatively known simply as axin is an important protein involved in cell signaling processes with a molecular weight of approximately 94 kDa. Axin 1 serves as a scaffold protein that plays a critical role in various signaling pathways. It shows expression in multiple tissues including the brain liver and kidney which indicates its involvement in various physiological processes. Due to its structural and functional properties axin 1 can be visualized and studied effectively even through techniques such as western blot.

Biological function summary

Axin 1 functions as a negative regulator within the Wnt signaling pathway by forming a destruction complex with other proteins. This complex is important in the degradation of beta-catenin thereby preventing excessive activation of Wnt target genes. It interacts with several components such as adenomatous polyposis coli (APC) and glycogen synthase kinase 3 beta (GSK3β) to facilitate these functions. The presence of axin 1 maintains the balance of signaling dynamics ensuring that cell proliferation and differentiation proceed correctly.

Pathways

Axin 1 plays a significant role in the control of the Wnt and JNK signaling pathways. Within the Wnt pathway it partners with proteins such as APC and GSK3β to regulate beta-catenin levels ensuring proper signal transduction. In the JNK pathway axin 1's interactions influence cell response to stress through modulation of signaling cascades. Its involvement in these pathways illustrates how axin 1 acts as a central component in cellular communication and response strategies.

Axin 1 associates with several conditions including colorectal cancer and hepatocellular carcinoma. Dysregulation of axin 1's function or expression can lead to aberrant Wnt signaling which is a pivotal factor in tumorigenesis linked with these cancers. Moreover proteins like beta-catenin and APC which interact with axin 1 also play a role in the progression of these diseases. Understanding axin 1's role provides insights into potential therapeutic strategies that target these pathways for effective treatment approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed : 12192039, PubMed : 27098453, PubMed : 28829046). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed : 12192039). In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (PubMed : 12192039). Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (PubMed : 16601693). Also a component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed : 17210684). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed : 17210684).

See full target information AXIN1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 9:7782-7792 PubMed38405482

2024

BIRC6 Modulates the Protein Stability of Axin to Regulate the Growth, Stemness, and Resistance of Renal Cancer Cells via the β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kaihua Zhong,Xiaohong Wang,Heyuan Zhang,Nanhui Chen,Yang Mai,Sipin Dai,Lawei Yang,Dong Chen,Weifeng Zhong

Cellular & molecular biology letters 27:92 PubMed36224534

2022

Targeting UBR5 in hepatocellular carcinoma cells and precise treatment via echinacoside nanodelivery.

Applications

Unspecified application

Species

Unspecified reactive species

Menghan Wang,Xing Ma,Guoyu Wang,Yanan Song,Miao Zhang,Zhongchao Mai,Borong Zhou,Ying Ye,Wei Xia

Frontiers in oncology 10:566637 PubMed33330038

2020

Comparative Assessment of the WNT/β-Catenin Pathway, CacyBP/SIP, and the Immunoproteasome Subunit LMP7 in Various Histological Types of Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Żaneta Piotrowska,Michał Niezgoda,Grzegorz Młynarczyk,Magdalena Acewicz,Irena Kasacka

Journal of thoracic disease 12:5906-5915 PubMed33209423

2020

WNT/β-catenin pathway activation via Wnt1 overexpression and Axin1 downregulation correlates with cadherin-catenin complex disruption and increased lymph node involvement in micropapillary-predominant lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhu,Shifeng Yang,Linfeng Zheng,Gu Zhang,Guoping Cheng

Journal of cellular biochemistry 119:8346-8358 PubMed29893431

2018

MicroRNA-539 promotes osteoblast proliferation and differentiation and osteoclast apoptosis through the AXNA-dependent Wnt signaling pathway in osteoporotic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong-Bai Zhu,Wen-Jun Lin,Chen Lv,Lu Wang,Zheng-Xiang Huang,Sheng-Wu Yang,Xin Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Axin 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (AB115205)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (AB115205)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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Secondary Antibodies

Primary Antibodies

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