Goat Polyclonal Axin 1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human AXIN1 aa 850 to C-terminus.
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Publications
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- Cellular & molecular biology letters 27:922022Targeting UBR5 in hepatocellular carcinoma cells and precise treatment via echinacoside nanodelivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMenghan Wang,Xing Ma,Guoyu Wang,Yanan Song,Miao Zhang,Zhongchao Mai,Borong Zhou,Ying Ye,Wei XiaPubMed 36224534
- Journal of thoracic disease 12:5906-59152020WNT/β-catenin pathway activation via Wnt1 overexpression and Axin1 downregulation correlates with cadherin-catenin complex disruption and increased lymph node involvement in micropapillary-predominant lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhu,Shifeng Yang,Linfeng Zheng,Gu Zhang,Guoping ChengPubMed 33209423
- Journal of cellular biochemistry 119:8346-83582018MicroRNA-539 promotes osteoblast proliferation and differentiation and osteoclast apoptosis through the AXNA-dependent Wnt signaling pathway in osteoporotic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiong-Bai Zhu,Wen-Jun Lin,Chen Lv,Lu Wang,Zheng-Xiang Huang,Sheng-Wu Yang,Xin ChenPubMed 29893431
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human AXIN1 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15169
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Baboon | Predicted |
| Chicken | Predicted |
| Cow | Predicted |
| Horse | Predicted |
| Rhesus monkey | Predicted |
| Zebrafish | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.75 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Chicken, Cow, Zebrafish, Baboon, Rhesus monkey | Dilution info - | Notes - |
Target data
Function
Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453, PubMed:28829046). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed:12192039). In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (PubMed:12192039). Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (PubMed:16601693). Also a component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed:17210684). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed:17210684).
Alternative names
AXIN, AXIN1, Axin-1, Axis inhibition protein 1, hAxin
Recommended products
Goat Polyclonal Axin 1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human AXIN1 aa 850 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human AXIN1 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15169
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Axin 1 alternatively known simply as axin is an important protein involved in cell signaling processes with a molecular weight of approximately 94 kDa. Axin 1 serves as a scaffold protein that plays a critical role in various signaling pathways. It shows expression in multiple tissues including the brain liver and kidney which indicates its involvement in various physiological processes. Due to its structural and functional properties axin 1 can be visualized and studied effectively even through techniques such as western blot.
- Biological function summary
Axin 1 functions as a negative regulator within the Wnt signaling pathway by forming a destruction complex with other proteins. This complex is important in the degradation of beta-catenin thereby preventing excessive activation of Wnt target genes. It interacts with several components such as adenomatous polyposis coli (APC) and glycogen synthase kinase 3 beta (GSK3β) to facilitate these functions. The presence of axin 1 maintains the balance of signaling dynamics ensuring that cell proliferation and differentiation proceed correctly.
- Pathways
Axin 1 plays a significant role in the control of the Wnt and JNK signaling pathways. Within the Wnt pathway it partners with proteins such as APC and GSK3β to regulate beta-catenin levels ensuring proper signal transduction. In the JNK pathway axin 1's interactions influence cell response to stress through modulation of signaling cascades. Its involvement in these pathways illustrates how axin 1 acts as a central component in cellular communication and response strategies.
- Associated diseases and disorders
Axin 1 associates with several conditions including colorectal cancer and hepatocellular carcinoma. Dysregulation of axin 1's function or expression can lead to aberrant Wnt signaling which is a pivotal factor in tumorigenesis linked with these cancers. Moreover proteins like beta-catenin and APC which interact with axin 1 also play a role in the progression of these diseases. Understanding axin 1's role provides insights into potential therapeutic strategies that target these pathways for effective treatment approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)
Immunohistochemical analysis of rat bladder tissue, staining Axin 1 with ab115205.
Tissue was fixed with formalin and blocked with 5000 μg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/400) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)
ab114205, at 3.75 ug/ml, staining Axin-1 in formalin-fixed,
paraffin-embedded Human adrenal tissue by immunohistochemistry.
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