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AB133221

Anti-Axin 1 antibody

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(2 Publications)

Rabbit Polyclonal Axin 1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AXIN1.

View Alternative Names

AXIN, AXIN1, Axin-1, Axis inhibition protein 1, hAxin

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (AB133221)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (AB133221)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human skin labelling Axin 1 with ab133221 at 5 ug/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human AXIN1. The exact immunogen used to generate this antibody is proprietary information.

O15169

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB109307

Anti-Axin 2 antibody [EPR2005(2)]

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  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Axin 1 alternatively known simply as axin is an important protein involved in cell signaling processes with a molecular weight of approximately 94 kDa. Axin 1 serves as a scaffold protein that plays a critical role in various signaling pathways. It shows expression in multiple tissues including the brain liver and kidney which indicates its involvement in various physiological processes. Due to its structural and functional properties axin 1 can be visualized and studied effectively even through techniques such as western blot.
Biological function summary

Axin 1 functions as a negative regulator within the Wnt signaling pathway by forming a destruction complex with other proteins. This complex is important in the degradation of beta-catenin thereby preventing excessive activation of Wnt target genes. It interacts with several components such as adenomatous polyposis coli (APC) and glycogen synthase kinase 3 beta (GSK3β) to facilitate these functions. The presence of axin 1 maintains the balance of signaling dynamics ensuring that cell proliferation and differentiation proceed correctly.

Pathways

Axin 1 plays a significant role in the control of the Wnt and JNK signaling pathways. Within the Wnt pathway it partners with proteins such as APC and GSK3β to regulate beta-catenin levels ensuring proper signal transduction. In the JNK pathway axin 1's interactions influence cell response to stress through modulation of signaling cascades. Its involvement in these pathways illustrates how axin 1 acts as a central component in cellular communication and response strategies.

Axin 1 associates with several conditions including colorectal cancer and hepatocellular carcinoma. Dysregulation of axin 1's function or expression can lead to aberrant Wnt signaling which is a pivotal factor in tumorigenesis linked with these cancers. Moreover proteins like beta-catenin and APC which interact with axin 1 also play a role in the progression of these diseases. Understanding axin 1's role provides insights into potential therapeutic strategies that target these pathways for effective treatment approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed : 12192039, PubMed : 27098453, PubMed : 28829046). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed : 12192039). In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (PubMed : 12192039). Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (PubMed : 16601693). Also a component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed : 17210684). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed : 17210684).
See full target information AXIN1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 19:35 PubMed38715121

2024

UBE2N promotes cell viability and glycolysis by promoting Axin1 ubiquitination in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yang,Weihua Chen,Tianyi Tao,Jun Zhang,Dehui Kong,Jidong Hao,Chao Yu,Guoqiang Liao,Hua Gong

Oncology reports 41:43-56 PubMed30365128

2018

PEA15 promotes liver metastasis of colorectal cancer by upregulating the ERK/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Tang,Wenjin Liang,Yong Liao,Zeming Li,Yan Wang,Chao Yan
View all publications
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Product promise

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