AB218023

Anti-Axin 2 antibody

Name: Anti-Axin 2 antibody (ab218023) | Abcam
Brand: Abcam Limited
SKU: AB218023
Price: 525 USD
Availability: InStock

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Rabbit Polyclonal Axin 2 antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human Axin-2 aa 350-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Axin-2, Axin-like protein, Axis inhibition protein 2, Conductin, Axil, AXIN2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (AB218023)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (AB218023)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling Axin 2 with ab218023 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (AB218023)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat lymph node tissue labeling Axin 2 with ab218023 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Axin-2 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2T1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Axin 2 also known as Axin Invasion is a protein belonging to the Axin family. It serves as a negative regulator of the Wnt signaling pathway. The molecular weight of Axin 2 is approximately 96 kDa. It is expressed in various tissues including the brain heart and skeletal muscle. Within cellular structures Axin 2 localizes to the cytoplasm where it interacts with other key proteins in its signaling pathways.

Biological function summary

Axin 2 functions as a scaffolding protein facilitating the assembly of multi-protein complexes. It forms part of the beta-catenin destruction complex which includes proteins like GSK-3β and APC. This complex plays a critical role in degrading beta-catenin an important player in the Wnt signaling pathway. Axin 2 therefore acts to downregulate signaling cascades that lead to cell proliferation and differentiation.

Pathways

Axin 2 is integral to the Wnt/β-catenin signaling pathway and also influences the JNK signaling pathway. By interacting with proteins like β-catenin and GSK-3β Axin 2 helps regulate gene expression initiatives important for developmental processes and cellular homeostasis. It acts as a check against unwarranted cellular proliferation maintaining balance within these signaling networks.

Axin 2 has significant implications in colorectal cancer and osteomalacia. Its regulation of the Wnt signaling pathway links it closely with the progression of these diseases. Mutations or dysregulation in Axin 2 can lead to the abnormal accumulation of β-catenin which disrupts cellular processes and promotes cancer development. Furthermore altered expression of Axin 2 in osteomalacia highlights its role in bone formation and mineralization processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.

See full target information Axin-2

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