Anti-Axin 2 antibody

• WB

Project

Western blot - Anti-Axin 2 antibody (AB32197)

ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.

All lanes:

Western blot - Anti-Axin 2 antibody (ab32197) at 1 µg/mL

All lanes:

AXIN2 partial recombinant tagged protein at 0.1 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution

Predicted band size: 93 kDa

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• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (AB32197)

IHC image of ab32197 stained area of normal tissue in a human colorectal cancer FFPE section. Section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 seconds at 125°C. Section was incubated with ab32197 at a dilution of 1 : 500 for 1h at room temperature and detected using an HRP conjugated polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• WB

Project

Western blot - Anti-Axin 2 antibody (AB32197)

All lanes:

Western blot - Anti-Axin 2 antibody (ab32197) at 1 µg/mL

Lane 1:

SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Colon (Mouse) Tissue Lysate at 10 µg

Lane 3:

HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate at 10 µg

Lane 4:

LS147T (Colon cancer cells) (Human) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

Predicted band size: 93 kDa

Observed band size: 32 kDa,75 kDa,94 kDa

false

Exposure time: 16min

• WB

CiteAb

Western blot - Anti-Axin 2 antibody (AB32197)

Axin 2 western blot using anti-Axin 2 antibody ab32197. Publication image and figure legend from Sun, L., Zhang, Y., et al., 2023, Nat Commun, PubMed 36765047.

ab32197 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab32197 please see the product overview.

Microbial BSH overexpression in B. fragilis potentiates CRC progression.a–h HFD-fed Cdx2Apcf/w mice were colonized with BF BSHlow or BF BSHhigh for 12 weeks (n = 11 mice for BF BSHlow; n = 12 mice for BF BSHhigh). Unconjugated bile acid profiles (a) and total unconjugated bile acid levels (b) in the colon contents. Mann–Whitney U test with two-sided (a). Two-tailed Student’s t test (b). The ileum (c) and colon (d) tumor incidence, and tumor numbers with different sizes (<4 mm2, >4 mm2 and the sum of both. Mann–Whitney U test with two-sided. e Total tumor numbers in the intestine. Two-tailed Student’s t test. f, g Representative pictures of colon (left), gross images of tumors (top right) in the colon and H&E staining (bottom right) of colon tumor sections. Scale bars : 1.5 mm (top right) and 100 μm (bottom right). h Relative mRNA levels of WNT target genes in colon non-tumor (NT) and tumor (T) tissues (n = 10 for BF BSHlow-NT; n = 12 for BF BSHhigh-NT; n = 8 for BF BSHlow-T; n = 10 for BF BSHhigh-T). Kruskal–Wallis test with Dunn’s post hoc test. i WB data of proteins involved in WNT signaling (n = 3 independent samples/group). j Representative IHC staining of Ki-67 (n = 8 independent slides for BF BSHlow; n = 9 independent slides for BF BSHhigh). Scale bars : 100 μm. Data are presented as mean values +/− SEM in (a–e, h). Source data are provided as a Source Data File for Fig. 5.

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