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Anti-Axin 2 antibody [EPR2005(2)] (ab109307) is a rabbit monoclonal antibody that is used to detect Axin 2 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 50 publications


Images

Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (AB109307), expandable thumbnail
  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (AB109307), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (AB109307), expandable thumbnail
  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (AB109307), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (AB109307), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Pig
Predicted
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000 - 1/2000
Notes

-

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.

Alternative names

Recommended products

Anti-Axin 2 antibody [EPR2005(2)] (ab109307) is a rabbit monoclonal antibody that is used to detect Axin 2 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 50 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2005(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-Axin 2 antibody [EPR2005(2)] (ab109307) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of Axin 2?


Anti-Axin 2 [EPR2005(2)] (ab109307) specifically detects a band for Axin 2 (UniProt: Q9Y2T1) at a molecular weight of 94kDa.

Recommended positive controls


WB: C6, PC-12, NIH/3T3, MCF7, SW480, and PC3 cell lysates.IHC-P: Human lung carcinoma tissue.ICC/IF: LnCap cells.

Trusted by the scientific community


Anti-Axin 2 [EPR2005(2)] (ab109307) was first used in a scientific publication in 2011 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists


Anti-Axin 2 [EPR2005(2)] (ab109307) has over 5 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR2005(2)] also available for your convenience:
ab109307, Carrier free - Anti-Axin 2 antibody [EPR2005(2)] - BSA and Azide free ab192230, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Axin 2 antibody [EPR2005(2)] ab310998, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Axin 2 antibody [EPR2005(2)] ab311122, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Axin 2 antibody [EPR2005(2)] ab311754, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-Axin 2 antibody [EPR2005(2)] ab313034, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Axin 2 antibody [EPR2005(2)] ab313235, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Axin 2 antibody [EPR2005(2)] ab320944



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Axin 2 also known as Axin Invasion is a protein belonging to the Axin family. It serves as a negative regulator of the Wnt signaling pathway. The molecular weight of Axin 2 is approximately 96 kDa. It is expressed in various tissues including the brain heart and skeletal muscle. Within cellular structures Axin 2 localizes to the cytoplasm where it interacts with other key proteins in its signaling pathways.

Biological function summary

Axin 2 functions as a scaffolding protein facilitating the assembly of multi-protein complexes. It forms part of the beta-catenin destruction complex which includes proteins like GSK-3β and APC. This complex plays a critical role in degrading beta-catenin an important player in the Wnt signaling pathway. Axin 2 therefore acts to downregulate signaling cascades that lead to cell proliferation and differentiation.

Pathways

Axin 2 is integral to the Wnt/β-catenin signaling pathway and also influences the JNK signaling pathway. By interacting with proteins like β-catenin and GSK-3β Axin 2 helps regulate gene expression initiatives important for developmental processes and cellular homeostasis. It acts as a check against unwarranted cellular proliferation maintaining balance within these signaling networks.

Associated diseases and disorders

Axin 2 has significant implications in colorectal cancer and osteomalacia. Its regulation of the Wnt signaling pathway links it closely with the progression of these diseases. Mutations or dysregulation in Axin 2 can lead to the abnormal accumulation of β-catenin which disrupts cellular processes and promotes cancer development. Furthermore altered expression of Axin 2 in osteomalacia highlights its role in bone formation and mineralization processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Unpurified ab109307 staining Axin 2 in 293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 16 hours. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307), expandable thumbnail

    Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution

    Lane 1: SW480 cell lysate at 20 µg

    Lane 2: C6 cell lysate at 20 µg

    Lane 3: PC-12 cell lysate at 20 µg

    Lane 4: NIH/3T3 cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 93 kDa

    Observed band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with unpurified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307), expandable thumbnail

    Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution

    Lane 1: SW480 cell lysate at 20 µg

    Lane 2: C6 cell lysate at 20 µg

    Lane 3: PC-12 cell lysate at 20 µg

    Lane 4: NIH/3T3 cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 93 kDa

    Observed band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with purified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307), expandable thumbnail

    Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    All lanes: Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution

    Lane 1: MCF7 cell lysates at 10 µg

    Lane 2: SW480 cell lysates at 10 µg

    Lane 3: PC3 cell lysates at 10 µg

    Predicted band size: 93 kDa

    Observed band size: 95 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with unpurified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/150) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)

    Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with purified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/150) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

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Product protocols

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