Anti-Axin 2 antibody [EPR2005(2)] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] - BSA and Azide free (AB192230)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with purified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109307).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] - BSA and Azide free (AB192230)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with unpurified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109307).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] - BSA and Azide free (AB192230)

Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with unpurified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor^® 488-conjugated goat anti-mouse IgG (1/500).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109307).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] - BSA and Azide free (AB192230)

Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with purified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor^® 488-conjugated goat anti-mouse IgG (1/500).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109307).

• ICC/IF

AbReview46275****

Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] - BSA and Azide free (AB192230)

Unpurified ab109307 staining Axin 2 in 293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 16 hours. An Alexa Fluor^® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109307).

This image is courtesy of an anonymous Abreview