Rabbit Recombinant Monoclonal Axin 2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Axin 2 antibody [YCA-R23454-50 H2L2] - BSA and Azide free (Detector) (AB288613), expandable thumbnail](https://content.abcam.com/products/images/axin-2-antibody-yca-r23454-50-h2l2-bsa-and-azide-free-detector-ab288613--sandwich-elisa-img427685.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Axin 2 antibody [YCA-R23454-50 H2L2] - BSA and Azide free (Detector) (AB288613)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
Alternative names
Axin-2, Axin-like protein, Axis inhibition protein 2, Conductin, Axil, AXIN2
Recommended products
Rabbit Recombinant Monoclonal Axin 2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- YCA-R23454-50 H2L2
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab288613 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Axin2. The recommended pair for sandwich ELISA is:
Capture: Anti-Axin 2 antibody [YCA-R23454-140 H2L1] - BSA and Azide free (Capture) ab288595, Human Axin2 Capture Antibody (unconjugated)
Detector: ab288613, Human Axin2 Detector Antibody (unconjugated)
The reference range value is 39.1-5000 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Axin 2 also known as Axin Invasion is a protein belonging to the Axin family. It serves as a negative regulator of the Wnt signaling pathway. The molecular weight of Axin 2 is approximately 96 kDa. It is expressed in various tissues including the brain heart and skeletal muscle. Within cellular structures Axin 2 localizes to the cytoplasm where it interacts with other key proteins in its signaling pathways.
- Biological function summary
Axin 2 functions as a scaffolding protein facilitating the assembly of multi-protein complexes. It forms part of the beta-catenin destruction complex which includes proteins like GSK-3β and APC. This complex plays a critical role in degrading beta-catenin an important player in the Wnt signaling pathway. Axin 2 therefore acts to downregulate signaling cascades that lead to cell proliferation and differentiation.
- Pathways
Axin 2 is integral to the Wnt/β-catenin signaling pathway and also influences the JNK signaling pathway. By interacting with proteins like β-catenin and GSK-3β Axin 2 helps regulate gene expression initiatives important for developmental processes and cellular homeostasis. It acts as a check against unwarranted cellular proliferation maintaining balance within these signaling networks.
- Associated diseases and disorders
Axin 2 has significant implications in colorectal cancer and osteomalacia. Its regulation of the Wnt signaling pathway links it closely with the progression of these diseases. Mutations or dysregulation in Axin 2 can lead to the abnormal accumulation of β-catenin which disrupts cellular processes and promotes cancer development. Furthermore altered expression of Axin 2 in osteomalacia highlights its role in bone formation and mineralization processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Axin 2 antibody [YCA-R23454-50 H2L2] - BSA and Azide free (Detector) (ab288613), expandable thumbnail](https://content.abcam.com/products/images/axin-2-antibody-yca-r23454-50-h2l2-bsa-and-azide-free-detector-ab288613--sandwich-elisa-img427685.jpg "Sandwich ELISA - Anti-Axin 2 antibody [YCA-R23454-50 H2L2] - BSA and Azide free (Detector) (ab288613)")
Sandwich ELISA - Anti-Axin 2 antibody [YCA-R23454-50 H2L2] - BSA and Azide free (Detector) (ab288613)
Sandwich ELISA of Human Axin2 Antibody Pair - BSA and Azide free ab288631 with the capture antibody Anti-Axin 2 antibody [YCA-R23454-140 H2L1] - BSA and Azide free (Capture) ab288595 dilution at 2 µg/mL and detector antibody ab288613 dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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