Anti-Axl antibody [7E10] - BSA and Azide free

• Flow Cyt

Lab

Flow Cytometry - Anti-Axl antibody [7E10] - BSA and Azide free (AB264492)

Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte, Left panel) / HeLa (Human cervix adenocarcinoma epithelial cell, Right panel) labeling Axl using Anti-Axl antibody [7E10] (ab130218) at a 0.974 μg/mL (Red) followed by Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at a 1/2000. Mouse monoclonal IgG isotype control (Black). The unlabeled control is cells without incubation with primary antibody or secondary antibody (Blue).

Negative control : Jurkat.

Gated on viable cells.

This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab130218).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Axl antibody [7E10] - BSA and Azide free (AB264492)

Immunofluorescent analysis of 4% methanol-fixed, 0.1% Triton X-100 permeabilized NCI-H1299 cells labeling Axl with ab130218 at 7.788 μg/mL, followed by Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on NCI-H1299 cell line.

Negative control : Jurkat (PMID : 28423548).

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab130218 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2 : ab179513 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab130218).

• WB

Lab

Western blot - Anti-Axl antibody [7E10] - BSA and Azide free (AB264492)

The molecular weight observed was in consistant with the literature (PMID : 19541935).

Negative control : Jurkat (PMID : 28423548).

Blocking/Dilution buffer : 5% NFDM/TBST.

This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab130218).

All lanes:

Western blot - Anti-Axl antibody [7E10] (<a href='/en-us/products/primary-antibodies/axl-antibody-7e10-ab130218'>ab130218</a>) at 1.947 µg/mL

Lane 1:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 2:

DU145 (human prostate carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 140 kDa,80 kDa

false

Exposure time: 180s