AB314097

Anti-AXL antibody [BLR222K] - BSA free

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Axl antibody. Suitable for Flow Cyt, ICC, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human AXL aa 1-450.

View Alternative Names

UFO, AXL, Tyrosine-protein kinase receptor UFO, AXL oncogene

7 Images

Supplier Data

Immunocytochemistry - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Detection of human AXL by immunocytochemistry. Sample : FFPE section of DU145 cells. Antibody : Rabbit antiAXL recombinant monoclonal antibody [BLR222K] (A700-222). Secondary : HRP-conjugated goat antirabbit IgG.

Flow Cytometry - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Detection of human AXL (shaded) in OVCAR8 cells by flow cytometry. Antibody : Rabbit anti-AXL recombinant monoclonal antibody [BLR222K] (A700-222) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Supplier Data

Immunocytochemistry - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Detection of human AXL by immunocytochemistry. Sample : FFPE section of HDLM-2 cells. Antibody : Rabbit anti-AXL recombinant monoclonal antibody [BLR222K] (A700-222). Secondary : HRP-conjugated goat antirabbit IgG.

Supplier Data

Immunoprecipitation - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-AXL recombinant monoclonal antibody [BLR222K] (A700-222) used for IP at 6 µl/mg lysate. AXL was also immunoprecipitated by an antibody against a different epitope of AXL (A302-167A) and a polyclonal antibody against the same epitope of AXL (A302-168A). For blotting immunoprecipitated AXL, A700-222 was used at 1 : 1000. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-AXL antibody [BLR222K] - BSA free (ab314097) at 1/1000 dilution

All lanes:

OVCAR-8 cells at 1 mg

false

Supplier Data

Immunocytochemistry - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Detection of mouse AXL by immunocytochemistry. Sample : FFPE section of AML12 cells. Antibody : Rabbit anti-AXL recombinant monoclonal antibody [BLR222K] (A700-222). Secondary : HRP-conjugated goat antirabbit IgG.

Supplier Data

Western blot - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Samples : Whole cell lysate (10 µg) from OVCAR-8, HEK293T, HeLa, Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody : Rabbit anti-AXL recombinant monoclonal antibody [BLR222K] (A700-222 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Western blot - Anti-AXL antibody [BLR222K] - BSA free (ab314097) at 1/1000 dilution

Lane 1:

OVCAR-8 Whole cell lysate at 10 µg

Lane 2:

HEK293T Whole cell lysate at 10 µg

Lane 3:

HeLa Whole cell lysate at 10 µg

Lane 4:

Jurkat Whole cell lysate at 10 µg

Lane 5:

786-O Whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Samples : Whole cell lysate (25 µg) from AML12, C2C12, CT26, TCMK-1, and 4T1 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-AXL recombinant monoclonal antibody [BLR222K] (A700-222 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-AXL antibody [BLR222K] - BSA free (ab314097) at 1/1000 dilution

Lane 1:

AML12 Whole cell lysate at 25 µg

Lane 2:

C2C12 Whole cell lysate at 25 µg

Lane 3:

CT26 Whole cell lysate at 25 µg

Lane 4:

TCMK-1 Whole cell lysate at 25 µg

Lane 5:

4T1 Whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG.

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR222K

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IP, Flow Cyt, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human AXL aa 1-450. The exact immunogen used to generate this antibody is proprietary information.

P30530

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Axl protein also known as AXL receptor tyrosine kinase plays a significant role in cell signaling. It has a molecular weight of approximately 98 kDa. Axl is a transmembrane receptor that is expressed in a variety of tissues including the immune system reproductive organs and the central nervous system. It is mainly recognized for transmitting signals from the extracellular matrix into the cytoplasm by binding with its ligand Gas6.

Biological function summary

Axl is important in mediating cell survival proliferation and migration. It functions as part of the TAM family of receptors which also includes Tyro3 and Mer. Axl often forms complexes with these receptors to facilitate efficient signaling. This interaction triggers phosphorylation events that activate downstream signaling proteins reinforcing its influence in cellular functions.

Pathways

Axl is deeply involved in the PI3K-Akt and MAPK signaling pathways which are central to cellular growth and survival. In these pathways Axl interacts closely with proteins like PI3K and Akt to regulate processes such as apoptosis and metabolism. Its interaction with these pathways places Axl as an important modulator of intracellular signaling cascades.

Axl has been implicated in cancer progression and immune response dysregulation. In many cancer types Axl overexpression links to tumor invasion metastasis and resistance to therapy. Moreover Axl’s role in autoimmune diseases becomes evident through its association with the Gas6 protein. Exploring these connections furthers understanding of how Axl's aberrant activity influences health and disease.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.. (Microbial infection) Promotes Zika virus entry in glial cells, Sertoli cells and astrocytes (PubMed : 28076778, PubMed : 29379210, PubMed : 31311882). Additionally, Zika virus potentiates AXL kinase activity to antagonize type I interferon signaling and thereby promotes infection (PubMed : 28076778). Interferon signaling inhibition occurs via an SOCS1-dependent mechanism (PubMed : 29379210).

See full target information AXL

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AXL antibody [BLR222K] - BSA free

Immunocytochemistry - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Flow Cytometry - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Immunocytochemistry - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Immunoprecipitation - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Immunocytochemistry - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Western blot - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Western blot - Anti-AXL antibody [BLR222K] - BSA free (AB314097)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Product promise