Name: Anti-Axl antibody [EPR21107]
SKU: ab215205
Rating: 2 (2 reviews)

Rabbit Recombinant Monoclonal Axl antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

Images

Western blot - Anti-Axl antibody [EPR21107] (AB215205), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product promiseTestedExpectedPredictedNot recommended

	WB
Human	Tested
Mouse	Tested
Rat	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

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Target data

See full target information AXL

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope. (Microbial infection) Promotes Zika virus entry in glial cells, Sertoli cells and astrocytes (PubMed:28076778, PubMed:29379210, PubMed:31311882). Additionally, Zika virus potentiates AXL kinase activity to antagonize type I interferon signaling and thereby promotes infection (PubMed:28076778). Interferon signaling inhibition occurs via an SOCS1-dependent mechanism (PubMed:29379210).

Alternative names

UFO, AXL, Tyrosine-protein kinase receptor UFO, AXL oncogene

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Rabbit Recombinant Monoclonal Axl antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR21107
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Axl protein also known as AXL receptor tyrosine kinase plays a significant role in cell signaling. It has a molecular weight of approximately 98 kDa. Axl is a transmembrane receptor that is expressed in a variety of tissues including the immune system reproductive organs and the central nervous system. It is mainly recognized for transmitting signals from the extracellular matrix into the cytoplasm by binding with its ligand Gas6.

Biological function summary

Axl is important in mediating cell survival proliferation and migration. It functions as part of the TAM family of receptors which also includes Tyro3 and Mer. Axl often forms complexes with these receptors to facilitate efficient signaling. This interaction triggers phosphorylation events that activate downstream signaling proteins reinforcing its influence in cellular functions.

Pathways

Axl is deeply involved in the PI3K-Akt and MAPK signaling pathways which are central to cellular growth and survival. In these pathways Axl interacts closely with proteins like PI3K and Akt to regulate processes such as apoptosis and metabolism. Its interaction with these pathways places Axl as an important modulator of intracellular signaling cascades.

Associated diseases and disorders

Axl has been implicated in cancer progression and immune response dysregulation. In many cancer types Axl overexpression links to tumor invasion metastasis and resistance to therapy. Moreover Axl’s role in autoimmune diseases becomes evident through its association with the Gas6 protein. Exploring these connections furthers understanding of how Axl's aberrant activity influences health and disease.

Product promise

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8 product images

Western blot - Anti-Axl antibody [EPR21107] (ab215205)
Western blot: Anti-Axl antibody [EPR21107] (ab215205) staining at 1:1000 dilution, shown in green; Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) shown in red.
ab215205 was shown to bind specifically to Axl. A band was observed at 140 kDa in wild-type HeLa cell lysates with no signal observed at this size in Axl CRISPR-Cas9 edited cell line Human AXL knockout HeLa cell line ab265392 (CRISPR-Cas9 edited cell lysate Human AXL knockout HeLa cell lysate ab257152). The band observed in the CRISPR-Cas9 edited lysate lane below 90 kDa is likely to represent a truncated form of Axl. This has not been investigated further and the functional properties of the gene product have not been determined.
Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution.
All lanes: Western blot - Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: AXL knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human AXL knockout HeLa cell line (Human AXL knockout HeLa cell line ab265392)
Lane 2: Western blot - Human AXL knockout HeLa cell lysate (Human AXL knockout HeLa cell lysate ab257152)
Lane 3: NCI-H1299 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 98 kDa
Observed band size: 80140 kDa
Western blot - Anti-Axl antibody [EPR21107] (ab215205)
Western blot: Anti-Axl antibody [EPR21107] (ab215205) staining at 1:1000 dilution, shown in green; Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) shown in red.
ab215205 was shown to bind specifically to Axl. A band was observed at 140 kDa in wild-type HeLa cell lysates with no signal observed at this size in Axl CRISPR-Cas9 edited cell line Human AXL knockout HeLa cell line ab261810 (CRISPR-Cas9 edited cell lysate Human AXL knockout HeLa cell lysate ab257151). The band observed in the CRISPR-Cas9 edited lysate lane below 90 kDa is likely to represent a truncated form of Axl. This has not been investigated further and the functional properties of the gene product have not been determined.
Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution.
All lanes: Western blot - Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Western blot - Human AXL knockout HeLa cell line (Human AXL knockout HeLa cell line ab261810)
Lane 2: Western blot - Human AXL knockout HeLa cell lysate (Human AXL knockout HeLa cell lysate ab257151)
Lane 2: AXL knockout HeLa cell lysate at 20 µg
Lane 3: NCI-H1299 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 98 kDa
Observed band size: 80140 kDa
Western blot - Anti-Axl antibody [EPR21107] (ab215205)
Lanes 1 - 4: Merged signal (red and green). Green - ab215205 observed at 135 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab215205 was shown to react with Axl in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab215205 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 40 µg
Lane 2: AXL knockout HeLa cell lysate at 40 µg
Lane 3: Wild-type HeLa cell lysate at 20 µg
Lane 4: AXL knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 98 kDa
Observed band size: 135 kDa
Western blot - Anti-Axl antibody [EPR21107] (ab215205)
Western blot: Anti-Axl antibody [EPR21107] (ab215205) staining at 1:1000 dilution, shown in green; Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) shown in red.
ab215205 was shown to bind specifically to Axl. A band was observed at 140 kDa in wild-type HeLa cell lysates with no signal observed at this size in Axl CRISPR-Cas9 edited cell line Human AXL knockout HeLa cell line ab261810 (CRISPR-Cas9 edited cell lysate Human AXL knockout HeLa cell lysate ab257151). The band observed in the CRISPR-Cas9 edited lysate lane below 90 kDa is likely to represent a truncated form of Axl. This has not been investigated further and the functional properties of the gene product have not been determined.
Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution.
All lanes: Western blot - Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: AXL knockout HeLa cell lysate at 20 µg
Lane 3: NCI-H1299 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 98 kDa
Observed band size: 140 kDa
Western blot - Anti-Axl antibody [EPR21107] (ab215205)
Blocking/Dilution buffer: 5% NFDM/TBST.
Negative control: Jurkat
The 140 kDa band corresponds to the full-length Axl, while the 80 kDa band could be the cleaved form of Axl (PMID: 7822279 and 19541935).
The apparent molecular mass is higher than predicted, likely due to glycosylation (PMID: 23629654).
All lanes: Western blot - Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution
Lane 1: DU 145 (human prostate carcinoma cell line) whole cell lysate at 20 µg
Lane 2: NCI-H1299 (human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 98 kDa
Observed band size: 140 kDa, 80 kDa
Exposure time: 29s
Western blot - Anti-Axl antibody [EPR21107] (ab215205)
Exposure time : Lane 1: 6 seconds; Lane 2: 3 seconds; Lane 3: 46 seconds; Lanes 4-5: 3 minutes; Lanes 6-7: 10 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The 140 kDa band corresponds to the full-length Axl, while the 80 kDa band could be the cleaved form of Axl (PMID: 7822279 and 19541935).
The apparent molecular mass is higher than predicted, likely due to glycosylation (PMID: 23629654).
All lanes: Western blot - Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution
Lane 1: Human skeletal muscle lysate at 20 µg
Lane 2: Rat muscle lysate at 10 µg
Lane 3: Mouse muscle lysate at 10 µg
Lane 4: 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate at 20 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 7: HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 98 kDa
Observed band size: 140 kDa, 80 kDa
Western blot - Anti-Axl antibody [EPR21107] (ab215205)
False colour image of Western blot: Anti-Axl antibody [EPR21107] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab215205 was shown to bind specifically to Axl. A band was observed at 135 kDa in wild-type A549 cell lysates with no signal observed at this size in AXL knockout cell line Human AXL knockout A549 cell line ab273744 (knockout cell lysate ab273780). To generate this image, wild-type and AXL knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: AXL knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human AXL knockout A549 cell line (Human AXL knockout A549 cell line ab273744)
Lane 3: A549 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 98 kDa
Observed band size: 135 kDa
Western blot - Anti-Axl antibody [EPR21107] (ab215205)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-Axl antibody [EPR19880] ab219651 exhibits low sensitivity in western blot. It is recommended to use Anti-Axl antibody [EPR23892-15] ab259831 or ab215205 as alternatives or to optimize experimental conditions by increasing the sample loading amount, using a lower antibody dilution ratio, and employing femtogram-level sensitivity substrates.
Lanes 1 - 3: Western blot - Anti-Axl antibody [EPR19880] (Anti-Axl antibody [EPR19880] ab219651) at 1/1000 dilution
Lanes 5 - 7: Western blot - Anti-Axl antibody [EPR23892-15] (Anti-Axl antibody [EPR23892-15] ab259831) at 1/1000 dilution
Lanes 9 - 11: Western blot - Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution
Lanes 1, 5 and 9: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg
Lanes 2, 6 and 9: HUVEC (human umbilical vein endothelial cell line) whole cell lysate 20 µg
Lanes 3, 7 and 11: A549 (Human lung carcinoma epithelial cell) whole cell lysate 20 µg
Lanes 4, 8 and 12: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 80 kDa, 140 kDa
Exposure time: 180s