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AB325301

AZdye 568 Anti-mEOS VHH antibody [1E8]

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Anti-mEOS VHH antibody - conjugated to AZdye 568. Single-domain antibody raised in Alpaca, suitable for ICC/IF.

  • Enhanced penetration in 3D tissue sections & whole-mount samples
  • Superior brightness and ideal for super resolution imaging
  • Reliable and quantitative readout in all fluorescence-based analyses
1 Images
Immunocytochemistry/ Immunofluorescence - AZdye 568 Anti-mEOS VHH antibody [1E8] (AB325301)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - AZdye 568 Anti-mEOS VHH antibody [1E8] (AB325301)

PFA-fixed Cos7 cells expressing a TOM70-nfmEOS fusion protein (nf : non-fluorescent) were stained with ab325301 at a 1/500 dilution. (A) Greyscale image of the staining performed with ab325301. A typical mitochondrial staining was observed. (B) False color representation of the image shown in A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). (C) The corresponding DAPI signal of the depicted section. (D) Merge of all channels. False color representation of the DAPI signal : blue; the mEOS signal : red.

Key facts

Host species

Alpaca

Clonality

Monoclonal

Clone number

1E8

Isotype

IgG

Conjugation

AZdye 568

Excitation/Emission

Ex: 578nm, Em: 602nm

Carrier free

No

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Lobophyllia hemprichii": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Features:

  • High affinity monovalent binding + two fluorophores per sdAb for brighter signals
  • Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information mEOS
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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