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AB325306

AZdye 568 Anti-mRuby VHH antibody [1F8]

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Anti-mRuby VHH antibody - conjugated to AZdye 568. Single-domain antibody raised in Alpaca, suitable for ICC/IF.

  • Enhanced penetration in 3D tissue sections & whole-mount samples
  • Superior brightness and ideal for super resolution imaging
  • Reliable and quantitative readout in all fluorescence-based analyses

View Alternative Names

Red fluorescent protein eqFP611, GFP-like chromoprotein

1 Images
Immunocytochemistry/ Immunofluorescence - AZdye 568 Anti-mRuby VHH antibody [1F8] (AB325306)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - AZdye 568 Anti-mRuby VHH antibody [1F8] (AB325306)

PFA-fixed Cos7 cells expressing a TOM70-nfmRuby fusion protein (nf : non-fluorescent) were stained with ab325306 at a 1/500 dilution. (A) Greyscale image of the staining performed with ab325306. (B) False color representation of the image shown in A is presented in red (coloring according to the excitation wavelength of the employed fluorophore). (C) The corresponding DAPI signal of the depicted section. (D) Merge of A and C. False color representation of A in red and C in blue.

Key facts

Host species

Alpaca

Clonality

Monoclonal

Clone number

1F8

Isotype

IgG

Conjugation

AZdye 568

Excitation/Emission

Ex: 578nm, Em: 602nm

Carrier free

No

Reacts with

Entacmaea quadricolor

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Entacmaea quadricolor": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Entacmaea quadricolor": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Features:

  • High affinity monovalent binding + two fluorophores per sdAb for brighter signals
  • Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pigment protein.
See full target information Red fluorescent protein eqFP611
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com