AB325289
AZdye 568 Anti-Syt1 VHH antibody [7D5]
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Anti-Syt1 VHH antibody - conjugated to AZdye 568. Single-domain antibody raised in Alpaca, suitable for ICC/IF.
- Enhanced penetration in 3D tissue sections & whole-mount samples
- Superior brightness and ideal for super resolution imaging
- Reliable and quantitative readout in all fluorescence-based analyses
View Alternative Names
Synaptotagmin-1, Synaptotagmin I, p65, SytI, Syt1
1 Images
![Immunocytochemistry/ Immunofluorescence - AZdye 568 Anti-Syt1 VHH antibody [7D5] (AB325289)](https://content.abcam.com/products/images/azdye-568-syt1-vhh-antibody-7d5-ab325289--immunocytochemistry-immunofluorescence-img453216.jpg/_jcr_content/renditions/webp-768.webp)
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - AZdye 568 Anti-Syt1 VHH antibody [7D5] (AB325289)
PFA-fixed hippocampal primary neuronal cultures from rats were stained with ab325289 at a 1/500 dilution. (A) Greyscale image of the staining performed with ab325289. (B) Greyscale image of the counterstaining performed with an anti-GFP single-domain antibody. (C) DAPI signal of the depicted section. (D) Merge of A and C. False color representation of A in red, B in magenta and C in blue.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
Product details
Features:
- High affinity monovalent binding + two fluorophores per sdAb for brighter signals
- Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.
Properties and storage information
Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
Product protocols
For this product, it's our understanding that no specific protocols are required. You can visit:
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Calcium sensor that participates in triggering neurotransmitter release at the synapse (PubMed : 2333096, PubMed : 30107533). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes.. (Microbial infection) Receptor for C.botulinum neurotoxin type B (BoNT/B, botB); interaction is improved in the presence of gangliosides (PubMed : 14504267, PubMed : 17167418, PubMed : 8144634). BoNT/B toxin binds to the membrane proximal vesicular domain of Syt1 (residues 32-51) (PubMed : 14504267, PubMed : 17167421).. (Microbial infection) Receptor for C.botulinum neurotoxin type G (BoNT/G, botG); unlike the case with BoNT/B, interaction is not improved in the presence of gangliosides (PubMed : 15123599, PubMed : 20219474). BoNT/G toxin binds to the vesicular domain of Syt1 (residues 32-53) (PubMed : 15123599, PubMed : 20219474).
Product promise
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