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AB84864

Anti-AZI1 antibody

2

(1 Review)

|

(11 Publications)

Rabbit Polyclonal AZI1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CEP131 aa 1000 to C-terminus.

View Alternative Names

AZI1, KIAA1118, CEP131, Centrosomal protein of 131 kDa, 5-azacytidine-induced protein 1, Pre-acrosome localization protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (AB84864)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (AB84864)

ab84864 at 1/250 dilution staining AZI1 in asynchronous HeLa cells by Immunocytochemistry, NBF-fixed. Detection : Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (AB84864)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (AB84864)

ab84864 at 1/250 dilution staining AZI1 in asynchronous HeLa cells by Immunocytochemistry, NBF-fixed. Detection : Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human CEP131 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UPN4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified using an epitope specific to AZI1 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AZI1 also known as 5-azacytidine-induced protein 1 is a protein involved in various cellular processes. It has a molecular weight of approximately 52 kDa. AZI1 is expressed in multiple tissues with higher expression levels reported in the testis and basal skin epidermis. This protein is known for its mechanistic role in the regulation of ciliogenesis contributing to the formation and function of cilia in the cell. While the precise mechanisms remain under study AZI1's presence in cilia is significant for its mechanical functions.
Biological function summary

The function of AZI1 is associated with its participation in the BBSome complex a group of proteins critical for proper ciliogenesis. It acts as an important player in ciliogenesis aiding in the assembly and maintenance of cilia which are hair-like structures on cells. Cilia play essential roles in cellular signaling and the transport of proteins and other molecules across cellular surfaces. AZI1 helps in the recruitment of proteins essential for the biogenesis and maintenance of these structures thereby ensuring the correct functioning of cilia-dependent processes.

Pathways

AZI1's involvement in ciliogenesis makes it integral to the Hedgehog signaling pathway which relies heavily on ciliary function for the transduction of signals that regulate cellular growth and differentiation. In addition AZI1 interacts with proteins such as DYF-2 a homolog of human IFT144 which also participates in ciliogenesis and is a component of intraflagellar transport linking AZI1 to the intraflagellar transport pathways necessary for maintaining cilia structure and function.

AZI1's association with ciliogenesis connects it to disorders like Bardet-Biedl syndrome (BBS) a condition characterized by defects in ciliary structure and function. The malfunctioning of AZI1 and related BBSome proteins disrupts normal cilia function contributing to the development of BBS which manifests in symptoms like vision loss and obesity. Furthermore mutations in AZI1 are also studied in the context of certain ciliopathies connecting it alongside proteins like BBS1 and BBS4 which are also involved in these syndromes. Understanding AZI1's role in these disorders enhances insights into therapeutic targets for cilia-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation (PubMed : 17954613, PubMed : 24185901). In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation (PubMed : 24121310). In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner (PubMed : 24121310, PubMed : 26616734). Acts also as a negative regulator of BBSome ciliary trafficking (PubMed : 24550735). Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail (By similarity). Required for optimal cell proliferation and cell cycle progression; may play a role in the regulation of genome stability in non-ciliogenic cells (PubMed : 22797915, PubMed : 26297806). Involved in centriole duplication (By similarity). Required for CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed : 26297806). Essential for maintaining proper centriolar satellite integrity (PubMed : 30804208).
See full target information CEP131
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed40396915

2025

A conserved role for centriolar satellites in translation of centrosomal and ciliary proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Pachinger,Jeroen Dobbelaere,Cornelia Rumpf-Kienzl,Shiviya Raina,Júlia Garcia-Baucells,Marina Sarantseva,Andrea Brauneis,Alexander Dammermann

Oncology letters 19:3145-3152 PubMed32218865

2020

CEP131 knockdown inhibits cell proliferation by inhibiting the ERK and AKT signaling pathways in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junying Wang,Xiaoping Yang,Shixin Han,Lizhi Zhang

Cell reports 30:1447-1462.e5 PubMed32023461

2020

Wnt3a Stimulation Promotes Primary Ciliogenesis through β-Catenin Phosphorylation-Induced Reorganization of Centriolar Satellites.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Lang Kyun,Sun-Ok Kim,Hee Gu Lee,Jeong-Ah Hwang,Joonsung Hwang,Nak-Kyun Soung,Hyunjoo Cha-Molstad,Sangku Lee,Yong Tae Kwon,Bo Yeon Kim,Kyung Ho Lee

Nature communications 10:4176 PubMed31519908

2019

Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.

Applications

Unspecified application

Species

Unspecified reactive species

Søs Grønbæk Holdgaard,Valentina Cianfanelli,Emanuela Pupo,Matteo Lambrughi,Michal Lubas,Julie C Nielsen,Susana Eibes,Emiliano Maiani,Lea M Harder,Nicole Wesch,Mads Møller Foged,Kenji Maeda,Francesca Nazio,Laura R de la Ballina,Volker Dötsch,Andreas Brech,Lisa B Frankel,Marja Jäättelä,Franco Locatelli,Marin Barisic,Jens S Andersen,Simon Bekker-Jensen,Anders H Lund,Vladimir V Rogov,Elena Papaleo,Letizia Lanzetti,Daniela De Zio,Francesco Cecconi

Cells 7: PubMed29932434

2018

Osmotic Stress Blocks Mobility and Dynamic Regulation of Centriolar Satellites.

Applications

Unspecified application

Species

Unspecified reactive species

Julie C Nielsen,Cathrine Nordgaard,Maxim A X Tollenaere,Simon Bekker-Jensen

Nature communications 9:2016 PubMed29789535

2018

Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.

Applications

Unspecified application

Species

Unspecified reactive species

Dragomir B Krastev,Stephen J Pettitt,James Campbell,Feifei Song,Barbara E Tanos,Stoyno S Stoynov,Alan Ashworth,Christopher J Lord

Translational oncology 9:392-402 PubMed27661404

2016

PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide.

Applications

ICC/IF

Species

Human

Lan B Hoang-Minh,Loic P Deleyrolle,Nariaki S Nakamura,Alexander K Parker,Regina T Martuscello,Brent A Reynolds,Matthew R Sarkisian

Nature communications 6:10075 PubMed26616734

2015

p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1.

Applications

IP

Species

Human

Maxim A X Tollenaere,Bine H Villumsen,Melanie Blasius,Julie C Nielsen,Sebastian A Wagner,Jiri Bartek,Petra Beli,Niels Mailand,Simon Bekker-Jensen

Journal of cell science 127:2910-9 PubMed24816561

2014

Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.

Applications

ICC/IF

Species

Unspecified reactive species

Christopher J Staples,Katie N Myers,Ryan D D Beveridge,Abhijit A Patil,Anna E Howard,Giancarlo Barone,Alvin J X Lee,Charles Swanton,Michael Howell,Sarah Maslen,J Mark Skehel,Simon J Boulton,Spencer J Collis

The EMBO journal 32:3029-40 PubMed24121310

2013

A new cellular stress response that triggers centriolar satellite reorganization and ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bine H Villumsen,Jannie R Danielsen,Lou Povlsen,Kathrine B Sylvestersen,Andreas Merdes,Petra Beli,Yun-Gui Yang,Chunaram Choudhary,Michael L Nielsen,Niels Mailand,Simon Bekker-Jensen
View all publications
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