Anti-AZI1 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (AB99379)

Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.

• IP

Unknown

Immunoprecipitation - Anti-AZI1 antibody (AB99379)

Detection of AZI1 by Western Blot of Immunprecipitate.
ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-AZI1 antibody (ab99379)

Predicted band size: 122 kDa

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• WB

Unknown

Western blot - Anti-AZI1 antibody (AB99379)

All lanes:

Western blot - Anti-AZI1 antibody (ab99379) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 122 kDa

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Exposure time: 30s

• WB

CiteAb

Western blot - Anti-AZI1 antibody (AB99379)

AZI1 western blot using anti-AZI1 antibody ab99379. Publication image and figure legend from Airik, R., Schueler, M., et al., 2016, PLoS One, PubMed 27224062.

ab99379 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab99379 please see the product overview.

SDCCAG8 associates with a characteristic set of proteins at the centrosome.(A) The Sdccag8 interacting proteins discovered by SILAC as centrosomal components belong to 4 functional groups, i.e. centriolar satellite components, endosomal vesicle components, tRNA synthesis complex proteins, and myosin type II motors involved in ciliogenesis. (B) Overview of the SDCCAG8 constructs used in this study. Numbering corresponds to amino acid positions. Gray boxes designate coiled-coil domains. (C) SDCCAG8 interacts with RABEP2, ERC1, and CEP131. FLAG-tagged full-length and truncation constructs of SDCCAG8 were immunoprecipitated from extracts of HEK293 cells transfected with each construct and analyzed by Western blot. Blots were probed for FLAG, or for endogenous RABEP2, ERC1, and CEP131 respectively. Only full-length FLAG-tagged SDCCAG8, but not truncated SDCCAG8 constructs co-immunoprecipitate RABEP2, ERC1 or CEP131, except for the C-terminal SDCCAG8 fragment that weakly co-immunoprecipitated RABEP2. (D) Western blot for endogenous proteins in whole cell lysates shows equal loading. (E) CEP131 co-immunoprecipitates FLAG-SDCCAG8. Lysates from cells transfected with FLAG-SDCCAG8 and with non-specific control siRNA or with siCEP131 were subjected to immunoprecipitation using anti-CEP131 antibodies or normal IgG. While anti-CEP131 antibodies co-immunoprecipitated FLAG-SDCCAG8 from control lysates, CEP131 knockdown abolished immunoprecipitation of FLAG-SDCCAG8, demonstrating specificity of the interaction.

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