Rabbit Polyclonal AZI1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CEP131 aa 1-50.
Images
Publications
Filter by
- Journal of oncology 2020:27524172020CEP131 Abrogates CHK1 Inhibitor-Induced Replication Defects and Is Associated with Unfavorable Outcome in Neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKiyohiro Ando et. al.PubMed 33014050
- Nature communications 10:41762019Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSøs Grønbæk Holdgaard et. al.PubMed 31519908
- The Journal of biological chemistry 294:9844-98572019USP9X-mediated deubiquitination of B-cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZesen Shang,Jiao Zhao,Qi Zhang,Cheng Cao,Shanshan Tian,Kai Zhang,Ling Liu,Lei Shi,Na Yu,Shangda YangPubMed 31073027
- Nature communications 8:148662017USP9X regulates centrosome duplication and promotes breast carcinogenesis.
- PloS one 11:e01560812016SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRannar Airik et. al.PubMed 27224062
- Journal of cell science 127:2910-92014Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.Applications:WBReactive species:Unspecified reactive speciesChristopher J Staples et. al.PubMed 24816561
- Journal of cell science 125:4770-92012The centriolar satellite protein Cep131 is important for genome stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher J Staples et. al.PubMed 22797915
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CEP131 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UPN4
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation (PubMed:17954613, PubMed:24185901). In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation (PubMed:24121310). In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner (PubMed:24121310, PubMed:26616734). Acts also as a negative regulator of BBSome ciliary trafficking (PubMed:24550735). Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail (By similarity). Required for optimal cell proliferation and cell cycle progression; may play a role in the regulation of genome stability in non-ciliogenic cells (PubMed:22797915, PubMed:26297806). Involved in centriole duplication (By similarity). Required for CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). Essential for maintaining proper centriolar satellite integrity (PubMed:30804208).
Alternative names
AZI1, KIAA1118, CEP131, Centrosomal protein of 131 kDa, 5-azacytidine-induced protein 1, Pre-acrosome localization protein 1
Recommended products
Rabbit Polyclonal AZI1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CEP131 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CEP131 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UPN4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AZI1 also known as 5-azacytidine-induced protein 1 is a protein involved in various cellular processes. It has a molecular weight of approximately 52 kDa. AZI1 is expressed in multiple tissues with higher expression levels reported in the testis and basal skin epidermis. This protein is known for its mechanistic role in the regulation of ciliogenesis contributing to the formation and function of cilia in the cell. While the precise mechanisms remain under study AZI1's presence in cilia is significant for its mechanical functions.
- Biological function summary
The function of AZI1 is associated with its participation in the BBSome complex a group of proteins critical for proper ciliogenesis. It acts as an important player in ciliogenesis aiding in the assembly and maintenance of cilia which are hair-like structures on cells. Cilia play essential roles in cellular signaling and the transport of proteins and other molecules across cellular surfaces. AZI1 helps in the recruitment of proteins essential for the biogenesis and maintenance of these structures thereby ensuring the correct functioning of cilia-dependent processes.
- Pathways
AZI1's involvement in ciliogenesis makes it integral to the Hedgehog signaling pathway which relies heavily on ciliary function for the transduction of signals that regulate cellular growth and differentiation. In addition AZI1 interacts with proteins such as DYF-2 a homolog of human IFT144 which also participates in ciliogenesis and is a component of intraflagellar transport linking AZI1 to the intraflagellar transport pathways necessary for maintaining cilia structure and function.
- Associated diseases and disorders
AZI1's association with ciliogenesis connects it to disorders like Bardet-Biedl syndrome (BBS) a condition characterized by defects in ciliary structure and function. The malfunctioning of AZI1 and related BBSome proteins disrupts normal cilia function contributing to the development of BBS which manifests in symptoms like vision loss and obesity. Furthermore mutations in AZI1 are also studied in the context of certain ciliopathies connecting it alongside proteins like BBS1 and BBS4 which are also involved in these syndromes. Understanding AZI1's role in these disorders enhances insights into therapeutic targets for cilia-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-AZI1 antibody (ab99379)
All lanes: Western blot - Anti-AZI1 antibody (ab99379) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Predicted band size: 122 kDa
Exposure time: 30s
Immunoprecipitation - Anti-AZI1 antibody (ab99379)
Detection of AZI1 by Western Blot of Immunprecipitate.
ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.All lanes: Immunoprecipitation - Anti-AZI1 antibody (ab99379)
Predicted band size: 122 kDa
Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99379)
Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-AZI1 antibody (ab99379)
AZI1 western blot using anti-AZI1 antibody ab99379. Publication image and figure legend from Airik, R., Schueler, M., et al., 2016, PLoS One, PubMed 27224062.
ab99379 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab99379 please see the product overview.
SDCCAG8 associates with a characteristic set of proteins at the centrosome.(A) The Sdccag8 interacting proteins discovered by SILAC as centrosomal components belong to 4 functional groups, i.e. centriolar satellite components, endosomal vesicle components, tRNA synthesis complex proteins, and myosin type II motors involved in ciliogenesis. (B) Overview of the SDCCAG8 constructs used in this study. Numbering corresponds to amino acid positions. Gray boxes designate coiled-coil domains. (C) SDCCAG8 interacts with RABEP2, ERC1, and CEP131. FLAG-tagged full-length and truncation constructs of SDCCAG8 were immunoprecipitated from extracts of HEK293 cells transfected with each construct and analyzed by Western blot. Blots were probed for FLAG, or for endogenous RABEP2, ERC1, and CEP131 respectively. Only full-length FLAG-tagged SDCCAG8, but not truncated SDCCAG8 constructs co-immunoprecipitate RABEP2, ERC1 or CEP131, except for the C-terminal SDCCAG8 fragment that weakly co-immunoprecipitated RABEP2. (D) Western blot for endogenous proteins in whole cell lysates shows equal loading. (E) CEP131 co-immunoprecipitates FLAG-SDCCAG8. Lysates from cells transfected with FLAG-SDCCAG8 and with non-specific control siRNA or with siCEP131 were subjected to immunoprecipitation using anti-CEP131 antibodies or normal IgG. While anti-CEP131 antibodies co-immunoprecipitated FLAG-SDCCAG8 from control lysates, CEP131 knockdown abolished immunoprecipitation of FLAG-SDCCAG8, demonstrating specificity of the interaction.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com