Rabbit Polyclonal AZI1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human Centrosomal protein of 131 kDa aa 1-50.
IgG
Rabbit
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Liquid
Polyclonal
IP | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Chimpanzee | Predicted | Predicted | Predicted |
Gorilla | Predicted | Predicted | Predicted |
Orangutan | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
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Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation (PubMed:17954613, PubMed:24185901). In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation (PubMed:24121310). In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner (PubMed:24121310, PubMed:26616734). Acts also as a negative regulator of BBSome ciliary trafficking (PubMed:24550735). Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail (By similarity). Required for optimal cell proliferation and cell cycle progression; may play a role in the regulation of genome stability in non-ciliogenic cells (PubMed:22797915, PubMed:26297806). Involved in centriole duplication (By similarity). Required for CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). Essential for maintaining proper centriolar satellite integrity (PubMed:30804208).
AZI1, KIAA1118, CEP131, AZI1, KIAA1118, Centrosomal protein of 131 kDa, 5-azacytidine-induced protein 1, Pre-acrosome localization protein 1
Rabbit Polyclonal AZI1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human Centrosomal protein of 131 kDa aa 1-50.
IgG
Rabbit
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
AZI1 also known as 5-azacytidine-induced protein 1 is a protein involved in various cellular processes. It has a molecular weight of approximately 52 kDa. AZI1 is expressed in multiple tissues with higher expression levels reported in the testis and basal skin epidermis. This protein is known for its mechanistic role in the regulation of ciliogenesis contributing to the formation and function of cilia in the cell. While the precise mechanisms remain under study AZI1's presence in cilia is significant for its mechanical functions.
The function of AZI1 is associated with its participation in the BBSome complex a group of proteins critical for proper ciliogenesis. It acts as an important player in ciliogenesis aiding in the assembly and maintenance of cilia which are hair-like structures on cells. Cilia play essential roles in cellular signaling and the transport of proteins and other molecules across cellular surfaces. AZI1 helps in the recruitment of proteins essential for the biogenesis and maintenance of these structures thereby ensuring the correct functioning of cilia-dependent processes.
AZI1's involvement in ciliogenesis makes it integral to the Hedgehog signaling pathway which relies heavily on ciliary function for the transduction of signals that regulate cellular growth and differentiation. In addition AZI1 interacts with proteins such as DYF-2 a homolog of human IFT144 which also participates in ciliogenesis and is a component of intraflagellar transport linking AZI1 to the intraflagellar transport pathways necessary for maintaining cilia structure and function.
AZI1's association with ciliogenesis connects it to disorders like Bardet-Biedl syndrome (BBS) a condition characterized by defects in ciliary structure and function. The malfunctioning of AZI1 and related BBSome proteins disrupts normal cilia function contributing to the development of BBS which manifests in symptoms like vision loss and obesity. Furthermore mutations in AZI1 are also studied in the context of certain ciliopathies connecting it alongside proteins like BBS1 and BBS4 which are also involved in these syndromes. Understanding AZI1's role in these disorders enhances insights into therapeutic targets for cilia-related diseases.
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All lanes: Western blot - Anti-AZI1 antibody (ab99379) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Predicted band size: 122 kDa
Exposure time: 30s
Detection of AZI1 by Western Blot of Immunprecipitate.
ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
All lanes: Immunoprecipitation - Anti-AZI1 antibody (ab99379)
Predicted band size: 122 kDa
Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
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