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AB157214

Anti-AZIN2 antibody [EPR7124(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal AZIN2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ADC, KIAA1945, ODCP, AZIN2, Antizyme inhibitor 2, AzI2, Arginine decarboxylase, Ornithine decarboxylase-like protein, ornithine decarboxylase paralog, ARGDC, ODC-like protein, ODC-p

2 Images
Flow Cytometry (Intracellular) - Anti-AZIN2 antibody [EPR7124(2)] (AB157214)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-AZIN2 antibody [EPR7124(2)] (AB157214)

Overlay histogram showing SH-SY5Y cells stained with ab157214 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab157214, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor®488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Western blot - Anti-AZIN2 antibody [EPR7124(2)] (AB157214)
  • WB

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Western blot - Anti-AZIN2 antibody [EPR7124(2)] (AB157214)

All lanes:

Western blot - Anti-AZIN2 antibody [EPR7124(2)] (ab157214) at 1/1000 dilution

All lanes:

Human cerebellum lysate at 10 µg

Predicted band size: 50 kDa

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  • Carrier free

    Anti-AZIN2 antibody [EPR7124(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7124(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target AZIN2 also known as Antizyme Inhibitor 2 is a protein that modulates polyamine levels within cells by inhibiting antizyme activity. It weighs approximately 47 kDa. AZIN2 expresses in various tissues including the brain and adrenal glands where its role is closely associated with polyamine biosynthesis. The protein interacts with antizymes to regulate their function which in turn controls the degradation of ornithine decarboxylase and the uptake of polyamines.
Biological function summary

AZIN2 functions as a modulator of the polyamine pathway preventing protein degradation and facilitating cellular growth and proliferation. It is part of a regulatory complex that includes antizymes and affects the homeostasis of polyamines. This complex interplay significantly impacts the cellular environment and plays a role in maintaining cellular function influencing processes such as signal transduction and ion transport.

Pathways

AZIN2's manipulation of the polyamine pathway reveals its role in cellular growth regulation. It participates in the pathway alongside the arginine and proline metabolism influencing the balance of polyamines within cells. AZIN2 shares a relationship with ornithine decarboxylase and antizymes stabilizing polyamine levels critical for DNA stabilization and protein synthesis.

Dysregulation of AZIN2 can relate to cancer and metabolic syndrome. In cancer disrupted polyamine regulation can lead to uncontrolled cell proliferation; AZIN2 affects this through its interaction with antizymes and ornithine decarboxylase. In metabolic syndrome altered polyamine levels associate with energy homeostasis and can implicate AZIN2’s function in disease progression. Understanding these connections provides potential therapeutic avenues for managing such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding (PubMed : 17900240). Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production (PubMed : 17900240). Participates in the morphological integrity of the trans-Golgi network (TGN) and functions as a regulator of intracellular secretory vesicle trafficking (PubMed : 20188728).
See full target information AZIN2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 131: PubMed33586680

2021

Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.

Applications

Unspecified application

Species

Unspecified reactive species

Leslie A Sandusky-Beltran,Andrii Kovalenko,Devon S Placides,Kevin Ratnasamy,Chao Ma,Jerry B Hunt,Huimin Liang,John Ivan T Calahatian,Camilla Michalski,Margaret Fahnestock,Laura J Blair,April L Darling,Jeremy D Baker,Sarah N Fontaine,Chad A Dickey,Joshua J Gamsby,Kevin R Nash,Erin Abner,Maj-Linda B Selenica,Daniel C Lee
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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